Navigation Links
Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies

e sample. This assay is highly sensitive and relatively fast (2 to 4 hours), but the required multistep operations of the assay are labor-intensive. A plate reader, which is best for quantitating ELISA results, may be an investment that is prohibitive for some laboratories.

A rapid method of isotyping uses anti-mouse antibody that is conjugated to an indicator molecule, such as colloidal gold or latex, and isotyping antibodies coated on a membrane in separate, discreet lines. The antibody in a sample forms an antigen-antibody complex with the indicator conjugate and is exposed to the isotyping membrane. When the sample complex meets the specific isotyping antibody, a colored line appears. Results are judged by reading the line at a specific location. This format allows multiple samples to be analyzed in a simple one-step operation. Fast results (5 minutes) and high sensitivity (as low as 1.0 g/ml of antibody, according to manufacturers) characterize this newest immunoglobulin isotyping method. Because this method is easy to use and gives rapid results, it is likely to replace the ELISA method for mouse immunoglobulin isotyping.

Single-Step Isotyping

figure 1

Stratagenes IsoDetect mouse monoclonal antibody isotyping kit is a rapid method for determining the isotypes of mouse monoclonal antibodies. This kit uses a colloidal-gold conjugation technique to label rabbit anti-mouse l and k light chain antibodies as indicating reagents, and rabbit anti-mouse isotyping immunoglobulin coated on a membrane. When a specific mouse immunoglobulin is present in a sample, a complex containing mouse immunoglobulin and rabbit anti-mouse l or k gold-conj
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
3. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
4. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
5. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
6. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
7. Highly Sensitive microRNA Array Performance
8. Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
9. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
10. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
11. Mouse Tail Genomic DNA Isolation Protocol(1)
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Simple Sensitive Isotyping Mouse Monoclonal Antibodies

(Date:4/24/2015)... N.J. , April 24, 2015  Navitas Life Sciences ... as Head of Clinical and Regulatory Services. Shalabh is ... been a co-founder and CEO of Kinapse, as well as ... Logo - http://photos.prnewswire.com/prnh/20150423/201261LOGO ... Jim Tizzard commented "I am delighted to welcome Shalabh ...
(Date:4/23/2015)... 23, 2015 The Thomas Brain ... conduct a groundbreaking pilot study to see if ... in as little as three to six months. ... and nutritional medicine—will aggressively target and simultaneously treat ... our aging brain. These include oxidative stress, neuroinflammation, ...
(Date:4/23/2015)... WuXi PharmaTech (Cayman) Inc. (NYSE: WX ... company serving the pharmaceutical, biotechnology and medical device industries, ... the United States , today announced ... quarter of 2015 after the New York Stock Exchange ... Thursday morning, May 14, 2015 Shanghai time). The earnings ...
(Date:4/23/2015)... April 23, 2015 Glemser ... the first EMC® Enterprise Content Division (ECD) Certified ... EMC Documentum® for Life Sciences Solution Suite ... knowledge and capabilities to provide life sciences companies ... , The EMC Documentum Solution Suite offers ...
Breaking Biology Technology:Shalabh Kumar Joins Navitas Life Sciences to Lead their Clinical and Regulatory Services Teams 2New Study Looks to Reverse Alzheimer’s Disease 2New Study Looks to Reverse Alzheimer’s Disease 3WuXi PharmaTech Schedules First-Quarter 2015 Earnings Release 2WuXi PharmaTech Schedules First-Quarter 2015 Earnings Release 3Glemser Earns Accreditation for Life Sciences Solution Suite 2
... Omeros Corporation (NASDAQ: OMER ), ... and commercializing products targeting inflammation, coagulopathies and disorders ... Gregory A. Demopulos, M.D., chairman and chief executive ... and Renshaw 13th Annual Healthcare Conference taking place ...
... , Gains US Patent Protection for Proprietary AAV Intellectual ... leader in the field of human gene therapy, today ... agreement with the US National Institutes of Health (NIH) ... therapy vectors for liver and brain indications. This agreement ...
... This September, during Prostate Cancer Awareness Month , ... method that may change the face of prostate ... and one Boston hospital, analyzed by the National Cancer Institute, ... Dr. David Samadi, Vice Chairman, Department of Urology, and ...
Cached Biology Technology:Amsterdam Molecular Therapeutics Licenses Additional Novel Gene Therapy Vectors from National Institutes of Health 2Amsterdam Molecular Therapeutics Licenses Additional Novel Gene Therapy Vectors from National Institutes of Health 3More Precise Prostate Cancer Screening on Horizon 2More Precise Prostate Cancer Screening on Horizon 3
(Date:4/13/2015)... , April 13, 2015 ... TechSci Research, "Global Biometrics Market Forecast & Opportunities, 2020", ... a CAGR of around 14% till 2020. The ... for large scale implementation and review of biometric ... launch of new products with greater efficiency, are ...
(Date:4/9/2015)... April 9, 2015 Synaptics Inc. (NASDAQ: SYNA ... today that it will report financial results for the ... 2015, after the close of market. The company will ... at 2:00 p.m. PT (5:00 p.m. ET), during which ... participate on the live call, analysts and investors should ...
(Date:4/2/2015)... 2, 2015 In the ... far outperforms the strongest competitors, showing outstanding performance ... the system is capable of a throughput of ... up to be ten times faster than most ... performance.      (Photo: http://photos.prnewswire.com/prnh/20150402/738673-a ...
Breaking Biology News(10 mins):Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2Synaptics to Report Third Quarter Results on April 23 2DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 2DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 3
... New research from The University of Western Ontario leads ... the Arctic Circle during the Pleistocene Epoch (approx. 150,000 ... three years later than modern day African elephants due ... nursing pattern could have contributed to the prehistoric elephant,s ...
... 21, 2010 Reportlinker.com announces that a new market ... Indian Biometric Market http://www.reportlinker.com/p0351944/Indian-Biometric-Market.html ... fastest emerging technology in the Indian Securities & Identification ... many conventional methods of Identity & Security checks like, ...
... Miscanthus x giganteus is the forerunner in the ... researchers believe "putting all their eggs in one basket" could ... recently reported the first natural occurrence in several decades of ... M. x giganteus is the only variety available, ...
Cached Biology News:Being good moms couldn't save the woolly mammoth 2New Miscanthus hybrid discovery in Japan could open doors for biofuel industry 2New Miscanthus hybrid discovery in Japan could open doors for biofuel industry 3
G-protein coupled purinergic receptor P2Y8...
This application changes sensitive keywords in an FCS file to remove patient information. It was contributed by Dr. David Miller....
Recognizes rat Taste Receptor 2 (TR2). The immunogen shows no significant sequence homology with TR1, gustducin or other pheromone receptors....
Rat monoclonal [HK5.3] to B7 RP1 (Biotin) ( Abpromise for all tested applications). entrezGeneID: 23308 SwissProtID: O75144...
Biology Products: