Navigation Links
Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies

edge of antibody structure directs the strategies for purifying immunoglobulins. For example, protein A affinity purification of mouse IgG molecules is widely used for single-step affinity purification. However, mouse IgG1 binds to protein A with only very low affinity. Therefore, purification of an IgG1 antibody is best carried out using a protein G resin.1

Isotyping Methods

Due to the importance of knowing the isotype of an immunoglobulin molecule, there are several methods currently available to determine the isotypes. The classic technique is the Ouchterlony (double diffusion) assay.2 This method uses a thin layer of agarose in which several wells are punched, usually in a circular pattern around a centered well. A sample is added in the central well and isotyping antiserum is added in the peripheral wells. As the sample and isotyping antibodies in the wells diffuse, specific isotyping antibody will meet the immunoglobulin sample and form a precipitate. The precipitation line that occurs between the sample well and an antiserum well indicates the isotype. This assay is inexpensive and simple to use. However, the sensitivity can be low, and 12 to 24 hours may be required to obtain results.

An alternative to the Ouchterlony assay is the more sensitive enzyme-linked immunosorbent (ELISA) method.3 The ELISA method uses a 96-well microtiter plate as a solid support for anti-mouse immunoglobulin antibodies that are coated onto each well. Sample mouse immunoglobulins in solution are added and captured by the anti-mouse antibodies. Specific anti-mouse isotyping antibodies are then introduced and allowed to bind to the mouse-anti-mouse antibody complex. Finally, an enzyme-tagged antibody that reacts specifically with the anti-isotyping antibodies is added, which, together with a colorimetric substrate, indicate the immunoglobulin isotype of th
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
3. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
4. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
5. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
6. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
7. Highly Sensitive microRNA Array Performance
8. Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
9. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
10. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
11. Mouse Tail Genomic DNA Isolation Protocol(1)
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Simple Sensitive Isotyping Mouse Monoclonal Antibodies

(Date:10/18/2014)... The Asian arthroscopic devices report defines ... and forecast of revenue. The arthroscopic devices market in ... 2018, at a CAGR of 6.5% from 2013 to ... arthroscopic devices market, to get an idea of the ... the segmentation of arthroscopic devices market in the same ...
(Date:10/18/2014)... York, NY (PRWEB) October 18, 2014 ... a healthy rate in the five years to 2014. ... several federal renewable energy tax credits that encouraged the ... have enacted renewable portfolio standards (RPSs), which require local ... percentage of their total energy portfolio. Increased campaigning for ...
(Date:10/17/2014)... Air Force Office of Scientific Research grant to examine ... that will provide clues for predicting fatigue in aircraft ... said the new technology and process would be better ... wing., "We,ll be able to determine metal fatigue at ... this scale," Huang said. "Certain patterns of surface roughness ...
(Date:10/17/2014)... PA (PRWEB) October 17, 2014 ... being called upon by health facilities around the ... patients are being treated. Among the many places ... includes Texas Health Presbyterian Hospital Dallas and at ... The state-of-the-art vapor-producing robots have been utilized to ...
Breaking Biology Technology:The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 2The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 3The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 4Biomass Power in the US Industry Market Research Report from IBISWorld Has Been Updated 2Biomass Power in the US Industry Market Research Report from IBISWorld Has Been Updated 3UT Arlington project to detect possible damages in aircraft parts early in process 2Bioquell’s Vapor Producing Robots Selected to Combat Ebola in Hospitals Around the World 2Bioquell’s Vapor Producing Robots Selected to Combat Ebola in Hospitals Around the World 3Bioquell’s Vapor Producing Robots Selected to Combat Ebola in Hospitals Around the World 4Bioquell’s Vapor Producing Robots Selected to Combat Ebola in Hospitals Around the World 5Bioquell’s Vapor Producing Robots Selected to Combat Ebola in Hospitals Around the World 6
... on its technology strengths, will its economic weaknesses eventually ... 21st century knowledge economy are impressive when compared with ... intimidating, especially when it comes to training and retaining ... skilled workers - from construction trades to laboratory technicians ...
... The past is a source of knowledge, and the future is ... S. Ambrose (1936-2002) Ernest Mueller , ... collections represent,a rich source of study material, much of it meticulously ... the field. While the majority of,this material was originally collected and ...
... , , ... NBS microbiologists recently assisted researchers at the University of ... strain of Pichia pastoris yeast. Top,yields by the researchers thus far ... in shake flasks. By switching to a BioFlo 3000 ...
Cached Biology Technology:To be a player in the "Knowledge Economy," Wisconsin needs more tech workers 2To be a player in the "Knowledge Economy," Wisconsin needs more tech workers 3To be a player in the "Knowledge Economy," Wisconsin needs more tech workers 4Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA) 2Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA) 3Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA) 4Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA) 5Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA) 6Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA) 7140-Fold Increase of Protein Achieved in Pichia Fermentation 2
(Date:10/16/2014)... wages war on the human body. Battles are ... In pancreatic cancer, this stalemate—known as tumor dormancy—can ... malignant, a phenomena that is poorly understood. ... laboratory of Salvatore Torquato, a Professor of Chemistry ... surrounding tumor dormancy and the switch to a ...
(Date:10/16/2014)... a new synthesis of recent research findings to inform ... the two states. , The Ecology and Management ... A Synthesis of the Relevant Biophysical Science and Implications ... by the U.S. Forest Service,s Pacific Northwest Research Station, ... for a synthesis of the large body of scientific ...
(Date:10/16/2014)... monocultures can be cultivated efficiently, they are anything ... caused by monoculture cultivation is becoming increasingly evident. ... crop form and are regarded as the sole ... – quite wrongfully, finds Bernhard Schmid, an ecology ... a novel form of agriculture and forestry. After ...
Breaking Biology News(10 mins):Modeling tumor dormancy 2New report synthesizes best available science on management of moist mixed-conifer forests 2Plant communities produce greater yield than monocultures 2Plant communities produce greater yield than monocultures 3
... A UC Riverside-led research team has found that as ... they do so by increasing only that segment of ... of an individual to contribute offspring to the next ... have studied for long, the researchers found that guppies ...
... to treat diseases such as cystic fibrosis, reduce bacterial ... and the University of Texas at Austin has determined ... be pivotal in preventing certain bacterial infections in plants, ... the Proceedings of the National Academy of Sciences. ...
... the prevailing belief that adult brain cells don't grow, a ... in the Dec. 27 issue of Public Library of Science ... occur in mature brains. , This finding means that ... to replace ones damaged by disease or spinal cord injury, ...
Cached Biology News:Fish evolve a longer lifespan by evolving a longer reproductive period, researchers find 2Scientists determine structure of enzyme that disrupts bacterial virulence 2MIT researcher finds neuron growth in adult brain 2MIT researcher finds neuron growth in adult brain 3
...
D.R. Harper and C. Grose (1994) • Includes: virus structure & replication, fundamental virology, immunology and pathogenesis, vaccines and antiviral drugs, novel diagnostic techniques, cloning a...
IHC Polymer Detection Kit (Rabbit DAB)150 slides...
...
Biology Products: