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Silencer Validated siRNAs

ed to cells transfected with an equal concentration of the Silencer Negative Control #1 (Ambion). Input cDNA in the different samples was normalized using real-time data for 18S rRNA. The bar graphs represent the average of two data points per siRNA concentration.


Advantages of Using Highly Effective siRNA Sequences
The use of highly effective siRNAs, such as those included in the Silencer Validated siRNA product line, has a number of advantages over using moderately active siRNAs:

* It's possible to transfect less siRNA than what is traditionally used (e.g., 100 nM) and still see the same effect. You can, therefore, perform more experiments with a given amount of siRNA.

* Because less siRNA is needed, experiments can be designed that use multiple siRNAs transfected into the same cell sample without causing cytotoxicity (e.g., toxicity is usually a problem when transfecting cells with greater than 200 nM siRNA).

* Most importantly, using sequences that have been verified to effectively induce gene silencing avoids the need to screen siRNAs, and thus saves time and money, allowing the researcher to focus on biological questions. An example of one type of experiment that is possible with the Silencer Validated siRNAs is described in "Using Validated siRNAs in Functional Genomics Assays". Of course, many other applications exist, including target validation studies and pathway analysis.

* A single, validated siRNA does not require experiments to determine which siRNA is the functional siRNA of a mixture and permits e
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Related biology technology :

1. pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
2. Selecting siRNA Sequences to Incorporate into the pSilencer Vectors
3. Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function
4. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
5. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
6. Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit
7. Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector
8. Using Validated siRNAs in Functional Genomic Assays
9. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
10. Five Ways to Produce siRNAs
11. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
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