Navigation Links
Silencer Validated siRNAs

ed to cells transfected with an equal concentration of the Silencer Negative Control #1 (Ambion). Input cDNA in the different samples was normalized using real-time data for 18S rRNA. The bar graphs represent the average of two data points per siRNA concentration.


Advantages of Using Highly Effective siRNA Sequences
The use of highly effective siRNAs, such as those included in the Silencer Validated siRNA product line, has a number of advantages over using moderately active siRNAs:

* It's possible to transfect less siRNA than what is traditionally used (e.g., 100 nM) and still see the same effect. You can, therefore, perform more experiments with a given amount of siRNA.

* Because less siRNA is needed, experiments can be designed that use multiple siRNAs transfected into the same cell sample without causing cytotoxicity (e.g., toxicity is usually a problem when transfecting cells with greater than 200 nM siRNA).

* Most importantly, using sequences that have been verified to effectively induce gene silencing avoids the need to screen siRNAs, and thus saves time and money, allowing the researcher to focus on biological questions. An example of one type of experiment that is possible with the Silencer Validated siRNAs is described in "Using Validated siRNAs in Functional Genomics Assays". Of course, many other applications exist, including target validation studies and pathway analysis.

* A single, validated siRNA does not require experiments to determine which siRNA is the functional siRNA of a mixture and permits e
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
2. Selecting siRNA Sequences to Incorporate into the pSilencer Vectors
3. Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function
4. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
5. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
6. Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit
7. Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector
8. Using Validated siRNAs in Functional Genomic Assays
9. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
10. Five Ways to Produce siRNAs
11. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Silencer Validated siRNAs

(Date:7/28/2015)... Md. and RESEARCH TRIANGLE PARK, N.C., July 28, 2015 ... today announced its financial results for the second ...  sales grew nearly 300% as compared to the ... first launched," said Roger Jeffs, Ph.D., United Therapeutics, ... Orenitram sales was due to an increase in ...
(Date:7/28/2015)... Israel , July 28, 2015 /PRNewswire/ ... regenerative medicine company utilizing its proprietary plant-based rhCollagen ... Shomrat Shurtz has been appointed Senior Director of ...      (Photo: http://photos.prnewswire.com/prnh/20150728/246955 ) , ... experience in marketing, regulatory and business strategy. Prior ...
(Date:7/28/2015)... July 28, 2015  Faced with increasing challenges ... palliative rather than symptomatic, the global healthcare industry ... therapies capable of curing or significantly changing the ... to look towards regenerative medicine as a viable ... paradigm in human health with the potential to ...
(Date:7/27/2015)... , July 27, 2015  Amgen (NASDAQ: ... second quarter financial results on Thursday, July 30, 2015, ... announcement will be followed by a conference call with ... the call from Amgen will be Robert A. ... members of Amgen,s senior management team. Live ...
Breaking Biology Technology:United Therapeutics Corporation Reports Second Quarter 2015 Financial Results 2United Therapeutics Corporation Reports Second Quarter 2015 Financial Results 3United Therapeutics Corporation Reports Second Quarter 2015 Financial Results 4United Therapeutics Corporation Reports Second Quarter 2015 Financial Results 5United Therapeutics Corporation Reports Second Quarter 2015 Financial Results 6United Therapeutics Corporation Reports Second Quarter 2015 Financial Results 7United Therapeutics Corporation Reports Second Quarter 2015 Financial Results 8United Therapeutics Corporation Reports Second Quarter 2015 Financial Results 9United Therapeutics Corporation Reports Second Quarter 2015 Financial Results 10United Therapeutics Corporation Reports Second Quarter 2015 Financial Results 11CollPlant Names Shomrat Shurtz Senior Director of Business Development 2Stem Cell Therapy to Redefine Regenerative Medicine, says Frost & Sullivan 2Stem Cell Therapy to Redefine Regenerative Medicine, says Frost & Sullivan 3Stem Cell Therapy to Redefine Regenerative Medicine, says Frost & Sullivan 4Stem Cell Therapy to Redefine Regenerative Medicine, says Frost & Sullivan 5Amgen Announces Webcast of 2015 Second Quarter Financial Results 2
... have invented a new technology to deposit "nanostructure films" on ... eyeglasses that cost less and work better. Ultimately, the ... efficiently produce energy. The films reduce the reflectance of ... light, reduce glare and also reduce exposure to ultraviolet light. ...
... In finally answering an elusive scientific question, researchers ... National Laboratory (Berkeley Lab) have shown that the ... phase and its spatial arrangement in correlated electron ... much attention now because they can display properties ...
... , , , ... SNSS ) reported today that it received a letter, ... of The NASDAQ Stock Market notifying Sunesis that it does not ... on The NASDAQ Capital Market set forth in NASDAQ Listing Rule ...
Cached Biology Technology:New nanostructure technology provides advances in eyeglass, solar energy performance 2Strain on nanocrystals could yield colossal results 2Strain on nanocrystals could yield colossal results 3Sunesis Pharmaceuticals Receives NASDAQ Notification 2Sunesis Pharmaceuticals Receives NASDAQ Notification 3
(Date:7/27/2015)... JOSE, Calif. , July 27, 2015 /PRNewswire/ ... developer of human interface solutions, today announced that ... ® touchscreen solution for its stylish smartwatch. ... for its proven reliability, low power and highly ... a wet finger. Huawei designers also required a ...
(Date:7/27/2015)... ANGELES , July 27, 2015   ... evidence- and experience-based clinical improvement solutions, today announced ... solution is now available on Android smartphones and ... inpatient and post-care organizations can use ZynxCarebook to ... same patient,s condition, streamline care transitions to other ...
(Date:7/23/2015)... -- Aware, Inc. (NASDAQ: AWRE ), a leading supplier ... its second quarter ended June 30, 2015.  ... a decrease of 33% compared to $6.8 million in the ... of 2015 was $0.3 million, or $0.01 per diluted share, ... in the same period a year ago.  ...
Breaking Biology News(10 mins):Synaptics Touchscreen Solution Powers New Huawei Watch 2Synaptics Touchscreen Solution Powers New Huawei Watch 3Zynx Health Adds Android Device Support to ZynxCarebook Solution 2Zynx Health Adds Android Device Support to ZynxCarebook Solution 3Aware, Inc. Reports Second Quarter 2015 Financial Results 2Aware, Inc. Reports Second Quarter 2015 Financial Results 3Aware, Inc. Reports Second Quarter 2015 Financial Results 4Aware, Inc. Reports Second Quarter 2015 Financial Results 5Aware, Inc. Reports Second Quarter 2015 Financial Results 6
... corals and adding stress to Caribbean coral reefs, which ... change, coral diseases, hurricanes, pollution, and overfishing. Restoring threatened ... pressure off the other corals, according to a new ... . The small, belligerently territorial, threespot damselfish kill ...
... May 26, 2010 UC Irvine scientists have created ... the first three-dimensional tissue structure to be made from ... toward the development of transplant-ready retinas to treat eye ... affect millions. "We made a complex structure consisting ...
... Studying whole microbial communities rather than individual micro-organisms could ... to climate change or pollution, says Dr Jack Gilbert writing ... Marine microbes are responsible for 99% of the cycling ... world,s primary productivity. "Given the vital role of ocean microbes ...
Cached Biology News:Distressed damsels stress coral reefs 2UCI researchers create retina from human embryonic stem cells 2Microbes answer more questions collectively 2
PURA Immunogen: PURA (NP_005850, 183 a.a. ~ 293 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Goat polyclonal to Glucose Dehydrogenase ( Abpromise for all tested applications). Antigen: Glucose Dehydrogenase (Bacillus species)...
Rabbit polyclonal to pCrosstide (HRP) ( Abpromise for all tested applications)....
Amyloid stain....
Biology Products: