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Silencer Validated siRNAs


Silencer Validated siRNAs are single siRNA duplexes that have been verified by real-time RT-PCR to reduce the expression of a number of important human genes. The cut-off for siRNA validation is a >70% reduction in target gene expression 48 hours post-transfection, though the average reduction in target gene expression in the inital set of validated siRNAs is greater than 90%. Every Silencer Validated siRNA strand is purified by HPLC, tested for purity by capillary electrophoresis or analytical HPLC, and verified for identity by MALDI-TOF mass spectometry. After annealing, the siRNAs are analyzed by nondenaturing gel or capillary electrophoresis to confirm that the strands are properly annealed. The result is the highest quality siRNA with a sequence verified to reduce gene expression.


Cenix and the Genome-Wide siRNA Library
The validated siRNAs described here are the first of what will be many new products stemming from Ambion's collaboration with Cenix BioScience. Cenix was founded in 1999 and pioneered the use of genome-wide dsRNA libraries by targeting all of the genes in the C. elegans and Drosophila genomes (1). Cenix was the first company to espouse the goal of developing siRNAs for the entire human genome. To achieve this goal, Cenix developed design parameters to create more efficacious siRNAs and high-throughput screening methods to enable the rapid validation of siRNA activity. The partnership of Cenix BioScience and Ambion will result in a continuous production of new validated siRNAs for the human genome and ensure that these siRNAs are produced with the highest quality standards for the entire research community.


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Related biology technology :

1. pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
2. Selecting siRNA Sequences to Incorporate into the pSilencer Vectors
3. Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function
4. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
5. YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs
6. Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit
7. Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector
8. Using Validated siRNAs in Functional Genomic Assays
9. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
10. Five Ways to Produce siRNAs
11. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
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