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Signal Transduction Reporting Systems Using Cis-Acting Enhancer,,,Elements

uction pathways. These vectors contain the luciferase reporter gene driven by a basic promoter element (TATA box) joined to tandem repeats of CRE, SRE, AP-1, NF-kB or SRF binding elements. When a cis-reporter plasmid and an experimental mammalian expression construct are cotransfected into mammalian cells, increased luciferase activity indicates transcription activation and the involvement of the gene product in signaling pathways converging at these cis-acting elements. The PathDetect in vivo signal transduction pathway cis- reporting systems can be used for studying the in vivo effects of a new gene, growth factor or drug candidate. When more specific assessment of a gene product is desired, the PathDetect trans-reporting systems can be used.

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