Navigation Links
Signal Transduction Reporting Systems Using Cis-Acting Enhancer,,,Elements

sily, inexpensively and quantitatively. Reporter enzyme assays replace tedious blotting procedures and are widely used due to their convenience, sensitivity and ability to generate reliable in vivo data.3

PathDetect Cis-Reporting Systems

figure 1

Each of the PathDetect in vivo signal transduction pathway cis-reporting systems includes one of five cis- reporter plasmids and a positive control plasmid. Positive control plasmids express gene products known to activate the signaling pathway converging at the corresponding enhancer elements. The five reporter plasmids (figure 1) of the PathDetect cis-reporting systems contain tandem repeats of one of the following enhancer elements: cyclic AMP response element (CRE), serum response element (SRE), nuclear factor kB (NF-kB), activator protein 1 (AP-1) or serum response factor (SRF). Together with a TATA box, these enhancer elements control the expression of the luciferase reporter gene located downstream.

Since most enhancer elements can be regulated by more than one transcription activator, each enhancer element can be used to monitor more than one signal transduction pathway. When a plasmid expressing a gene of interest is cotransfected into mammalian cells along with one of these cis-reporter plasmids, increased luciferase activity indicates transcription activation. The PathDetect cis-reporting systems are useful tools for assessing the potential involvement of a gene product in a pathway. For more specific assessment, the PathDetect trans-reporting systems can be used.4

Response to Overexpression of Known Activators


'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
2. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
3. In Vivo Signal Transduction Pathway Reporting Systems
4. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
5. New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways
6. Determining the Signal-to-Noise Ratio and Optimal Photomultiplier gain setting in the GenePix 4000B
7. Versatile Reporter Vectors for Monitoring Viral Transduction
8. Improved Vectors for PathDetect Trans-Reporting Systems
9. Cis-Reporting System Monitors Intracellular Calcium Mobilization
10. Cis-Reporting System Monitors Intracellular Calcium Mobilization
11. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
Post Your Comments:
(Date:9/1/2015)... WI (PRWEB) , ... September 01, 2015 , ... In ... Venice expertly transports the reader to a not-so-distant future faced with an Ebola pandemic ... beautiful professional who overcomes personal tragedy to enter the world of counterterrorism, and who ...
(Date:9/1/2015)... ... 2015 , ... Knowledgent , the data and analytics firm, today announced ... data lakes in the healthcare industry. , As the Internet of Things (IoT) continues ... the last few years as more entities become technology enabled through Electronic Medical Records ...
(Date:9/1/2015)... ... September 01, 2015 , ... GenoCAD, a ... groundbreaking new version of its flagship DNA design platform, which has been enhanced ... sequences into numerous genetic parts, which in turn can be recombined using specific ...
(Date:8/31/2015)... SAN DIEGO , Aug. 31, 2015 ... drug development company announces that the Company,s lead antibody, ... subject of five separate presentations during the upcoming World ... Hawaii September 2-5, 2015. Researchers from the ... (MSK) will present results on the use of MabVax,s ...
Breaking Biology Technology:Alexi Venice’s Debut Novel Takes on the Human Testing of a Vaccine for Ebola and Revenge by an Anti-Big Pharma Terrorist Group in ‘Ebola Vaccine Wars' 2Alexi Venice’s Debut Novel Takes on the Human Testing of a Vaccine for Ebola and Revenge by an Anti-Big Pharma Terrorist Group in ‘Ebola Vaccine Wars' 3New White Paper Explores Data Lake Opportunities in the Healthcare Industry 2GenoCAD Releases Combinatorial Design Tool to Optimize Protein Expression and Streamline DNA Synthesis Orders 2MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 2MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 3MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 4
... 2012   Spherix Incorporated (NASDAQ: SPEX ... diabetes, metabolic syndrome and atherosclerosis, and provider of technical ... pharmaceutical companies, today announced that it has entered into ... its common stock and warrants to purchase shares of ...
... Cambrex Corporation (NYSE: CBM ) announced ... on Monday, February 6, 2012 after the market closes. ... call to discuss the fourth quarter 2011 financial results.  ... Tuesday, February 7, 2012 at 8:30 a.m. Eastern Time ...
... strength of spider silk, the robust nature of the ... tears and winds that exceed hurricane strength. Now, ... with complex computer simulations has shown that web durability ... the overall web design compensates for damage and the ...
Cached Biology Technology:Spherix Announces Registered Direct Offering of $1.15 Million 2Spherix Announces Registered Direct Offering of $1.15 Million 3A spider web's strength lies in more than its silk 2A spider web's strength lies in more than its silk 3A spider web's strength lies in more than its silk 4
(Date:8/20/2015)... , Aug. 20, 2015 The ... consumers that are active and healthy. However, wearable ... the hospital environment to help improve diagnostic capabilities ... video from the Wearable Technologies Conference 2015 ... new applications for wearables in healthcare.    ...
(Date:8/18/2015)... , Aug. 17, 2015 Research ... addition of the "Global Biometric Authentication & ... - Estimation & Forecast, 2015-2020" report to ... authentication and identification systems market is expected to ... 2015 to 2020 and generate over $25 billion ...
(Date:8/12/2015)... , Aug. 12, 2015  Synaptics Inc. (NASDAQ: ... interface solutions, today announced that four of its ... solution, have officially been named FIDO Certified™ by ... of the certification, Synaptics, Natural ID™ fingerprint solutions ... Authentication Framework (UAF) standard and are interoperable among ...
Breaking Biology News(10 mins):Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 2Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 3Global Biometric Authentication & Identification Market 2015: Focus On Modality, Motility & Application - Estimation & Forecasts to 2020 for the $25 Billion Industry 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 3
... 26 The Peter and Patricia Gruber Foundation International Prize ... with an event to be held at Rockefeller University in ... and Neuroscience Prize recipients will be announced live at the ... Brain, and Society." The discussion will feature a panel ...
... at the way that dolphins, whales and porpoises scythe ... maximum aquatic agility. However, no one had ever analysed ... lift that they generate, the drag that they experience ... from Duke University teamed up with Mark Murray from ...
... investigators in the Vanderbilt Center for Structural Biology have ... structure of the largest membrane-spanning protein to date. , ... pictures" of small proteins, large proteins and particularly ... been reluctant to smile for the camera. , In ...
Cached Biology News:NYC science symposium marks Gruber Prize program 10th anniversary 2Dolphins get a lift from delta wing technology 2Structural biology scores with protein snapshot 2Structural biology scores with protein snapshot 3
... , CapSure HS and CapSure Macro LCM ... of populations of pure cells from tissue ... Microdissection., Ensure the Integrity of Microdissected ... CapSure LCM Caps have a patented transfer ...
Opticon continuous fluorescence detector; includes optical base, analysis software, computer and monitor (this is an accessory for the DNA Engine thermal cyclers which must be ordered seperately)...
... on one plate. The new ArraySlide 96 ... (7mm x 7mm) individual printing surfaces for ... analysis studies. Unlike other systems, this device ... presence of contaminating adhesives. The chamber itself ...
... chambers for sealing hybridization reactions on slides. The ... on a glass slide. Reaction solution is added ... are then sealed with plastic sealing dots. Dots ... of reaction fluid. These chambers enclose a 20 ...
Biology Products: