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Setting up the laboratory to avoid contamination

Always use new and/or sterilized glassware, plasticware, and pipettes to prepare PCR reagents and template DNA.
  • Autoclave all reagents and solutions that can be autoclaved without affecting their performance. Of course, primers, dNTPs and Taq DNA Polymerase should not be autoclaved.
    Have your own set of PCR reagents and solutions that are used only for PCR. Store these reagents in small aliquots.
  • When pipetting DNA, avoid creating aerosols that could carry contaminants.
  • Always include control reactions, for example a negative (no DNA) control which contains all reaction components except the template DNA, and a positive control that has been successfully used in previous PCRs.

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