Navigation Links
Setting up a kinase profiler with IMAP

230 151 40/60/ 1:1500 73 229 0/100/ 1:1500

Note to Table 2: The SF Tyr kit assay well ID, peptide sequence, and part number /name of peptide are shown. The Binding Solutions for the titration assay and for the SF Tyr assays are also shown. Binding Solutions are listed as % Buffer A/% Buffer B/dilution of Binding Reagent. For the FAK titration assay, the Binding Solutions were optimized slightly from the SF Tyr protocol in order to reduce the FP background and maximize the FP signal.

Serial dilutions of FAK were then tested with the three top substrates ranked by DmP from Figure 6, Panel B. Results of the enzyme titration assay are shown in Figure 7, and Table 2 lists the peptide sequences and their names and well IDs. Two of the top peptide hits with 0.02 U/mL are presently peptides in MDC's inventory; these are the FAM-Srctide and FAM-Src-Family-tide (well IDs A11B12 and G13H14, respectively). As expected the SF Tyr assay with the lesser FAK concentration was a more accurate predictor of EC50 than the 0.07 U/mL assay. The FAK substrate that ranked the best in terms of '"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Related biology technology :

1. Setting up the laboratory to avoid contamination
2. Reduce Errors and Save Time When Setting Up PCRs
3. Setting up Successful siRNA Library Screens
4. LiquiChip Kits for bead-based cytokine and kinase assays
5. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
6. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Setting kinase profiler with IMAP

(Date:9/17/2014)... , 17 de septiembre de 2014 /PRNewswire/ ... principal proveedor mundial de soluciones logísticas avanzadas ... de la vida, anunció hoy la contratación ... global con sede en Rótterdam, Países Bajos, ... clientes Europa en rápido crecimiento. Según los ...
(Date:9/17/2014)... 2014 First antibacterial ... a truly novel class of anti-infective in ... a biopharmaceutical company developing a truly novel ... has secured £4.0m ($6.4m) to advance the ... a range of Gram-positive infections including  Clostridium ...
(Date:9/17/2014)... The Alliance for Clinical ... non-profit collaborative building a global system to enhance ... announced Phase 3 of its Site Accreditation and ... H. Kalali, Vice-President, Global Head, Neuroscience Center of ... and Quality Integration (CQI) Consulting will lead the ...
(Date:9/16/2014)... energy from jaw movements has been created by a ... that the device can generate electricity from eating, chewing ... or wearable electronic devices, such as hearing aids, cochlear ... first results of the device,s performance have been published ... Materials and Structures . , Jaw movements have proved ...
Breaking Biology Technology:Cryoport contrata a Broekman Group para mejorar su apoyo operativo y logístico en Europa 2Cryoport contrata a Broekman Group para mejorar su apoyo operativo y logístico en Europa 3Cryoport contrata a Broekman Group para mejorar su apoyo operativo y logístico en Europa 4Cryoport contrata a Broekman Group para mejorar su apoyo operativo y logístico en Europa 5MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 2MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 3MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 4MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 5MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 6MGB Biopharma Secures £4.0m ($6.4m) to Advance its Novel Lead Antibacterial, MGB-BP-3, into Clinical Development 7Global Standards Initiative for Clinical Research Sites Advances 2Global Standards Initiative for Clinical Research Sites Advances 3Global Standards Initiative for Clinical Research Sites Advances 4'Smart material' chin strap harvests energy from chewing 2
... United,Therapeutics Corporation (Nasdaq: UTHR ) announced today ... results before the open of market,on Thursday, May ... half-hour teleconference on Thursday,May 1, 2008, at 9:00 ... 1-800-603-1777, with international callers dialing 1-706-679-8129.,A rebroadcast of ...
... 2008 / b3c newswire / - Sloning BioTechnology ... on a project to synthesize a gene mutant library ... and controlled ratio at preselected positions within a given ... Slonomics™, a unique DNA engineering platform using defined sets ...
... Duke University chemists have found a way to grow ... very large numbers, removing a major roadblock in ... carbon nanotubes also follow parallel paths as they grow ... electronic performance, said Duke associate chemistry professor Jie Liu, ...
Cached Biology Technology:Sloning and the Kornberg group at Stanford University starting evaluation project regarding the synthesis of a Statistically Randomized Gene Library 2Nanotubes grown straight in large numbers 2Nanotubes grown straight in large numbers 3
(Date:9/17/2014)... State,s Institute for the Arts and Humanities has received ... for a two-year project, "The Boundaries of the Human ... project will support a wide-ranging series of events aimed ... humanities. One involves artists and researchers who are exploring ... the idea of humanism, and the other involves the ...
(Date:9/17/2014)... (Sept. 17, 2014) A rare genetic disorder known as ... a recent joint investigation by researchers at San Diego ... In addition to suggesting better treatment options for people ... into the genetic underpinnings of autism., Jacobsen syndrome affects ... Institutes of Health. It occurs in a person when ...
(Date:9/17/2014)... the Copenhagen Centre for Social Evolution and Yale University ... birth weight and -length can partially predict the likelihood ... autism and schizophrenia later in life. The study analyzed ... hospital diagnoses for up to 30 years, and adjusted ... is published today in the Proceedings of the ...
Breaking Biology News(10 mins):Mellon Foundation awards grant for major project in the humanities and sciences 2A link between Jacobsen syndrome and autism 2Size at birth affects risk of adolescent mental health disorders 2Size at birth affects risk of adolescent mental health disorders 3
... scientists has produced a new type of rice that ... rice crops. Professor Andy Pereira at the Virginia Bioinformatics ... Indonesia, Israel, Italy, Mexico and The Netherlands to identify, ... HARDY that improves key features of this important grain ...
... WI, Sept. 18, 2007 -- Recently, researchers at Iowa ... of a popular crop model. By zeroing in on ... they can help improve yield predictions across a variety ... findings in the September-October issue of Crop Science. ...
... Metagenomics is a revolutionary approach to study microbes. Rather ... is applied directly to environmental samples to obtain information ... a specific habitat studied. The ocean is an ideal ... whose biomass equals that of all other living organisms ...
Cached Biology News:HARDY rice: less water, more food 2HARDY rice: less water, more food 3Simulating kernel production influences maize model accuracy 2Metagenomics of the deep Mediterranean 2
Rabbit polyclonal to GABA A Receptor gamma 2 ( Abpromise for all tested applications). entrezGeneID: 2566 SwissProtID: P18507...
Rabbit polyclonal to VPAC2 ( Abpromise for all tested applications). entrezGeneID: 7434 SwissProtID: P41587...
Anti-GOSR2 Monoclonal AntibodyOther Sub-Family: not assigned-Other Applications: Immunocytochemistry, Immunoprecipitation, Western Blot...
Mouse monoclonal [4A2.2] to SLC6A4 ( Abpromise for all tested applications). entrezGeneID: 6532 SwissProtID: P31645...
Biology Products: