Navigation Links
Setting up a kinase profiler with IMAP

L with specific activity of 1000 units per mg provides 40 units/mL, where 1 unit is defined as 1 nmol phosphate incorporated into substrate per minute.

Step 2. An EC50 concentration (predetermined by enzyme titration assay) of each kinase is suggested for the screen set up in Table B. Prepare a working stock of 150 mL of 4X the EC50 concentration for each kinase on one plate.


Prepare the ATP Working Solutions
Step 1. To make an 800 M ATP working solution, add 0.16 mL of a 10 M stock of ATP per 1.84 mL of Complete Reaction Buffer.

Step 2. To make an 80 M ATP working solution, add 0.1 mL of the 800 M solution prepared in Step 4A to 0.9 mL of Complete Reaction Buffer.

Step 3. The resulting dilutions of 800 and 80 M are 8X the final reaction concentrations of 100 and 10 M ATP.


Prepare the Substrate Working Solutions
For this assay, there are 10 different substrate solutions to prepare. Refer to Table A for details.

Step 1. Prepare each 20 M Fluorescent Substrate solution in Reaction Buffer with no DTS. Aliquot and store substrates at -20C to asssure stability for future use.

Step 2. To make an 800 nM substrate working solution, add 20 L of each 20 M solution per 480 L of Complete Reaction Buffer. Aliquot 3 x 150 L per microtube. This solution is 8X the final reaction concentration of 100 nM substrate.


B: SET UP KINASE REACTION
Add components to the 384-well assay plate according to the plate diagram in Table B

'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Related biology technology :

1. Setting up the laboratory to avoid contamination
2. Reduce Errors and Save Time When Setting Up PCRs
3. Setting up Successful siRNA Library Screens
4. LiquiChip Kits for bead-based cytokine and kinase assays
5. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
6. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Setting kinase profiler with IMAP

(Date:8/19/2014)... Pa. , Aug. 19, 2014 A ... has been nominated for a Centers for Disease Control ... addressing a major public health concern. ... System, is one of a team of researchers nominated ... Charles C. Shepard Science Award for Data Methods and ...
(Date:8/19/2014)... Aug. 19, 2014 Research ... of the "Spectroscopy Equipment and Accessories ... to their offering. This ... Equipment and Accessories in US$ Thousands by ... spectrophotometers, Raman Spectrometers, NMR-EPR Spectrometers, Near Infrared ...
(Date:8/19/2014)... AZ (PRWEB) August 19, 2014 Sales ... launch of its online sales skills training course ... sales skills. , MedTech clinical staff spend the majority ... a hospital, standalone medical center, or physician practice – ... staff. But why stop there? Sales Horizons believes clinical ...
(Date:8/18/2014)... Aug. 18, 2014   Sterne, Kessler, Goldstein & ... Washington, DC , announced today that ... Review (IPR) with the U.S. Patent and Trademark ... of BioReference Laboratories, Inc. (Nasdaq: BRLI).  These IPR petitions ... by Myriad Genetics, Inc. These patents have been asserted ...
Breaking Biology Technology:Geisinger investigator nominated for federal award 2Geisinger investigator nominated for federal award 3Global Spectroscopy Equipment and Accessories - Strategic Business Report 2014 2Sales Horizons Launches a New Online Sales Training Course for MedTech Clinical Staff to Develop Their Sales Skills 2Sales Horizons Launches a New Online Sales Training Course for MedTech Clinical Staff to Develop Their Sales Skills 3Sterne Kessler Files 11 IPR Petitions On Behalf Of GeneDx, Inc., Subsidiary Of BioReference Laboratories, Inc. 2Sterne Kessler Files 11 IPR Petitions On Behalf Of GeneDx, Inc., Subsidiary Of BioReference Laboratories, Inc. 3
... , , SANTA CLARA, Calif., Sept. 8 ... Board: APNO) today announced that they have entered into a ... Cell Biosciences for $1.50 per share or approximately $17.9 million ... analysis business to include a global infrastructure and a broad ...
... LAIYANG, China, Sept. 8 /PRNewswire-Asia-FirstCall/ -- Jiangbo,Pharmaceuticals, ... pharmaceutical company with its principal operations in the People,s,Republic ... year 2009. The Company expects to meet or ... and operating income of $40 to $43 million for ...
... , , ... systems biology tools company, announced today that Proteostasis Therapeutics has ... Therapeutics is the first company dedicated to the discovery of ... Network. The Proteostasis Network maintains the body,s natural balance ...
Cached Biology Technology:Cell Biosciences Signs Agreement to Acquire Alpha Innotech 2Cell Biosciences Signs Agreement to Acquire Alpha Innotech 3Cell Biosciences Signs Agreement to Acquire Alpha Innotech 4Cell Biosciences Signs Agreement to Acquire Alpha Innotech 5Jiangbo Pharmaceuticals Reaffirms Guidance for Fiscal 2009 2GeneGo Licenses MetaCore to Proteostasis Therapeutics 2
(Date:8/20/2014)... visible in in this image of the Para and ... were most likely intentionally set in order to deforest ... or stand of trees where the land is thereafter ... conversion of forestland to farms, ranches, or urban use. ... of the Amazon Rainforest in the middle of the ...
(Date:8/20/2014)... acral melanomas the rare type of skin cancer that ... from other more common types of skin cancer, according to ... Pigment Cell & Melanoma Research . , Acral melanoma most ... feet, nail-beds and other hairless parts of the skin. Unlike ... UV damage from the sun. , The team, from the ...
(Date:8/20/2014)... During the past few years, Virginia Tech,s Wu Feng ... Microsoft grant from the "Computing in the Cloud" program, ... a $6 million award from the Air Force on ... from NSF and the National Institutes of Health on ... wove together the "parallel computing" aspects from each grant, ...
Breaking Biology News(10 mins):Scientists learn more about rare skin cancer that killed Bob Marley 2The internet was delivered to the masses; parallel computing is not far behind 2The internet was delivered to the masses; parallel computing is not far behind 3The internet was delivered to the masses; parallel computing is not far behind 4
... 2008) A new study by the Bronx Zoo-based ... the Greater Yellowstone Ecosystem have apparently hopped into oblivion. ... of the journal Oryx, also speculates that the disappearance ... a variety of other prey species and their predators. ...
... crucial proteins in our bodies must fold into complex shapes ... to move them around, to speed up important chemical reactions ... "off" to affect which proteins our cells make. Recently, ... genetic code into protein-can act a lot like a protein ...
... of shrimp and fish in tropical coastal areas is ... poverty, but in fact this cultivation has negative consequences ... Daniel A. Bergquist of Uppsala University, Sweden, has studied ... The cultivating of fish and shellfish in artificial ...
Cached Biology News:Hareless: Yellowstone's rabbits have vanished, study says 2Stanford researchers make first direct observation of 3-D molecule folding in real time 2Stanford researchers make first direct observation of 3-D molecule folding in real time 3Stanford researchers make first direct observation of 3-D molecule folding in real time 4Stanford researchers make first direct observation of 3-D molecule folding in real time 5Global trade in tiger shrimp threatens environment 2
... Rat Antibody to Human CD66 ... is expressed by human neutrophils and ... carcinoma. Clone YTH71.3 reacts with transfectants ... are CEA subfamily members(2). ...
This STAT antibody sampler pack contains 8 monoclonal ,STAT antibodies....
Request Info...
Invitrogen's Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into tissue culture cells via calcium phosphate co-precipitation....
Biology Products: