Navigation Links
Setting up a kinase profiler with IMAP

Setting up a Kinase Profiler with IMAP for further details. For kinase selectivity profiling, the screen can be set up in a wide variety of formats. It can be designed to test a single candidate inhibitor or activator compound with different concentrations of ATP, titrate the drug candidate with each enzyme, titrate each enzyme with a single concentration of the compound, test multiple compounds with a single ATP concentration and a single kinase concentration, or other protocols may be devised.

Profiling experiments may vary depending on the type of compounds being tested. For example, if the drug candidates to be profiled are known to be ATP-competitive inhibitors, it may be useful to test with two or more concentrations of ATP. To facilitate profiling experiments, kinases with similar KmATPs can be tested together. For determination of the apparent KmATP for each kinase, please refer to IMAP Application Note #3, Determination of Ki and Km values in IMAP Assays, at www. moleculardevices.com.

For our IMAP Profiler, we tested the EC50 concentration of each kinase with both 10 M and 100 M ATP. Three known kinase inhibitors (H89, SB203580 and staurosporine) were tested with this IMAP Profiler. The plate set-up is shown in Table B. IMAP Reaction Buffer containing 0.01% Tween 20 rather than 0.1% BSA as a carrier was utilized in this Profiler to avoid inhibitor binding to BSA. This type of binding could reduce the effective inhibitor concentration and result in a right-shifted IC50 curve. Although assays listed in the following protocol that utilize the 100% B
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Related biology technology :

1. Setting up the laboratory to avoid contamination
2. Reduce Errors and Save Time When Setting Up PCRs
3. Setting up Successful siRNA Library Screens
4. LiquiChip Kits for bead-based cytokine and kinase assays
5. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
6. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Setting kinase profiler with IMAP

(Date:5/21/2015)... 21, 2015 Specialty Pharmacy Times ... been admitted to BPA Worldwide as a business ... track audience data for Specialty Pharmacy Times based ... “By becoming a member of BPA Worldwide, Specialty ... our clients with the most reliable, unequaled data,” ...
(Date:5/21/2015)... May 21, 2015 uBiome, the ... a partnership with PicnicHealth, a healthcare company that ... diagnosed with Inflammatory Bowel Disease (IBD) will receive ... complementary uBiome research kit. Both companies were funded ... , For more information on this partnership ...
(Date:5/21/2015)... 2015 W. R. Grace & ... in Worms, Germany has received good manufacturing practice ... the International Pharmaceutical Excipient Council (IPEC) Foundation. ... its SYLOID® FP brand of pharmaceutical grade excipient ... the Curtis Bay, Maryland (USA) and Sorocaba, Brazil ...
(Date:5/21/2015)... As part of its philanthropic partnership with Banner ... funded a $3.25 million project to relocate and equip ... up-to-date features and technology. , The new pharmacy is ... members will begin moving from the current location to ... , The current pharmacy has been using a ...
Breaking Biology Technology:Specialty Pharmacy Times Joins BPA Worldwide 2uBiome Partners with PicnicHealth 2uBiome Partners with PicnicHealth 3Grace European Facility Receives GMP Excipient Certification for SYLOID® FP Silica Gel 2Sun Health Foundation Provides $3.25M to Expand, Relocate Banner Del E. Webb Medical Center Pharmacy 2Sun Health Foundation Provides $3.25M to Expand, Relocate Banner Del E. Webb Medical Center Pharmacy 3
... Gov. Jim Doyle has announced that Berbee ... Fitchburg Technology Campus, will receive $250,000 in state Technology Zone ... in a new 43,250-square-foot office building and a 51,500-square-foot data ... the Fitchburg Technology Campus. , ,The project is expected ...
... Madison company that is developing drugs to protect cancer ... has closed on a Series A Preferred Stock financing ... , ProCertus BioPharm , an oncology-based pharmaceutical company, will ... patients to demonstrate proof-of-concept in humans for its chemo- ...
... a patient presents to a doctor, many assessments have to ... of what is unglamorously called managing the patient. , ,During ... would have us believe, one of the most important judgment ... or not. This is not in the sense that ...
Cached Biology Technology:ProCertus BioPharm secures $2.3M in financing 2ProCertus BioPharm secures $2.3M in financing 3Barriers will not stop convergence of medical technologies 2Barriers will not stop convergence of medical technologies 3Barriers will not stop convergence of medical technologies 4Barriers will not stop convergence of medical technologies 5Barriers will not stop convergence of medical technologies 6Barriers will not stop convergence of medical technologies 7
(Date:4/27/2015)... , Apr. 27, 2015 Profile Solutions, Inc. ... and security systems is pleased to announce that Dr ... a consultant and member of its scientific advisory board. ... known as a thought leader in technology-enhanced learning models. ... Group, and has published studies and books focused on ...
(Date:4/14/2015)... NEW YORK , April 14, 2015 /PRNewswire/ ... Fast IDentity Online (FIDO ® ) Alliance tm ... standards-based specifications. FIDO members commit to share technology ... authentication methods that are interoperable, more secure and ... specifications enable biometric identity verification that protects sensitive ...
(Date:4/9/2015)... Calif. , April 9, 2015 Synaptics ... interface solutions, announced today that it will report financial ... Thursday, April 23, 2015, after the close of market. ... analysts and investors at 2:00 p.m. PT (5:00 p.m. ... To participate on the live call, analysts ...
Breaking Biology News(10 mins):Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 2Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 3Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 4HYPR Corp. Joins the FIDO Alliance 2HYPR Corp. Joins the FIDO Alliance 3Synaptics to Report Third Quarter Results on April 23 2
... some parts of the brain when small stones are scattered ... study by researchers at UC Davis. The brains of those ... the wild, and the fish also showed behavior closer to ... between the hatchery and the wild fish," said graduate student ...
... of Sciences Kristineberg Marine Research Station are using the brittlestar ... to do experiments that avoid the ethical issues associated with ... is a close relative of the starfish and can regenerate ... Mike Thorndyke are presenting their latest work on Friday 7th ...
... of genes responsible for establishing the bilateral body axes in ... to play an unexpected role in patterning an animal with ... anemones, along with corals and jellyfish, are members of the ... is distinct from the familiar bilateral body plan exhibited by ...
Cached Biology News:How to grow a bigger brain 2Anemone genes reveal versatile building blocks for body plans 2