Navigation Links
Setting up a kinase profiler with IMAP

Setting up a Kinase Profiler with IMAP for further details. For kinase selectivity profiling, the screen can be set up in a wide variety of formats. It can be designed to test a single candidate inhibitor or activator compound with different concentrations of ATP, titrate the drug candidate with each enzyme, titrate each enzyme with a single concentration of the compound, test multiple compounds with a single ATP concentration and a single kinase concentration, or other protocols may be devised.

Profiling experiments may vary depending on the type of compounds being tested. For example, if the drug candidates to be profiled are known to be ATP-competitive inhibitors, it may be useful to test with two or more concentrations of ATP. To facilitate profiling experiments, kinases with similar KmATPs can be tested together. For determination of the apparent KmATP for each kinase, please refer to IMAP Application Note #3, Determination of Ki and Km values in IMAP Assays, at www. moleculardevices.com.

For our IMAP Profiler, we tested the EC50 concentration of each kinase with both 10 M and 100 M ATP. Three known kinase inhibitors (H89, SB203580 and staurosporine) were tested with this IMAP Profiler. The plate set-up is shown in Table B. IMAP Reaction Buffer containing 0.01% Tween 20 rather than 0.1% BSA as a carrier was utilized in this Profiler to avoid inhibitor binding to BSA. This type of binding could reduce the effective inhibitor concentration and result in a right-shifted IC50 curve. Although assays listed in the following protocol that utilize the 100% B
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Related biology technology :

1. Setting up the laboratory to avoid contamination
2. Reduce Errors and Save Time When Setting Up PCRs
3. Setting up Successful siRNA Library Screens
4. LiquiChip Kits for bead-based cytokine and kinase assays
5. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
6. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Setting kinase profiler with IMAP

(Date:5/23/2013)...  Rahal Letterman Lanigan Racing (RLL) and StemCyte, Inc., ... banking and therapeutics, announced today that StemCyte has become ... Indy car driven by James Jakes ... IndyCar 2013 season.   StemCyte, Inc. and Rahal ... of umbilical cord blood banking and its educational initiative ...
(Date:5/23/2013)... PI’s new LPS-24 positioners are ... mm travel range with closed-loop sensor resolution up ... these new positioners the ideal choice for high ... version is also available. , More Information: ... be found at: , http://www.physikinstrumente.com/en/products/prdetail.php?sortnr=1000745&prw-lps24 , ...
(Date:5/23/2013)... May 23, 2013  Saddleback Memorial Medical Center in ... the first hospital on the West Coast to perform ... surgical option provides women with a state-of-the-art, minimally invasive ... Marc Winter , M.D., a highly regarded ... medical director of minimally invasive surgery at Saddleback Memorial, ...
(Date:5/23/2013)... 23, 2013 BioTrends Research Group, one ... for specialized biopharmaceutical issues, finds that, unaided, one ... specialists reported that in the past six months, ... treatment) in anticipation of the next generation of ... when only 6 percent reported that they had ...
Breaking Biology Technology:StemCyte, Inc. Joins Rahal Letterman Lanigan Racing As Associate Sponsor Of No. 16 IndyCar Program For James Jakes Beginning With The 97th Indianapolis 500 2StemCyte, Inc. Joins Rahal Letterman Lanigan Racing As Associate Sponsor Of No. 16 IndyCar Program For James Jakes Beginning With The 97th Indianapolis 500 3Precision Positioning System Uses Miniaturezed Piezo Linear Motor: LPS-24 Linear Stage by PI 2Saddleback Memorial Surgeon First on the West Coast to Perform Single-Site Robotic Hysterectomy 2Saddleback Memorial Surgeon First on the West Coast to Perform Single-Site Robotic Hysterectomy 3The Majority of Physicians that Treat Hepatitis C Virus (HCV) Have Begun "Warehousing" and Preparing Their HCV Patients for the Next Generation of HCV Treatments 2The Majority of Physicians that Treat Hepatitis C Virus (HCV) Have Begun "Warehousing" and Preparing Their HCV Patients for the Next Generation of HCV Treatments 3
... International, LLC,(BioEnergy), a privately-held, biotech-based developer and ... the,acquisition of certain assets of OmniGene Bioproducts, ... employees. OBI, currently based in Cambridge,MA, possesses ... metabolic pathway engineering expertise to develop efficient ...
... to Provide an Attractive Domestic Alternative to ... 18 Fulcrum BioEnergy, Inc.,today announced that ... plans to build one of the first ... to ethanol. The plant will,process municipal solid ...
... China, July 18 /Xinhua-PRNewswire-FirstCall/ --,China-Biotics, Inc. (OTC ... Chinese firm specializing in the manufacture,research, development, ... it has applied for listing approval on ... an important milestone for China-Biotics, and we ...
Cached Biology Technology:BioEnergy International, LLC Acquires Assets of OmniGene Bioproducts, Inc. 2BioEnergy International, LLC Acquires Assets of OmniGene Bioproducts, Inc. 3Fulcrum BioEnergy Announces Plans to Build One of the First Commercial-Scale Ethanol Plants Using Municipal Solid Waste as Feedstock 2Fulcrum BioEnergy Announces Plans to Build One of the First Commercial-Scale Ethanol Plants Using Municipal Solid Waste as Feedstock 3China-Biotics, Inc. Files Application for Nasdaq Listing 2
(Date:5/23/2013)... Plant Science , Automated conserved ... and promoter evolution among grasses , Within the ... of DNA that undergo little change in position and ... for any proteins. Some of these evolutionarily stable sequences, ... the expression of other genes or the condensation of ...
(Date:5/23/2013)... RICHLAND, Wash. Pacific Northwest National Laboratory honored ... and commercialization of intellectual property at PNNL,s annual ... , The Department of Energy national laboratory ... Year for his work developing battery materials that ... to the electrical grid, and reduce the time ...
(Date:5/23/2013)... of Public Health at Emory University, along with ... received a $4 million grant over four years ... (Health and Exposome Research Center: Understanding Lifetime Exposures). ... awarded in the United States. , The ... of Environmental Health Sciences (NIEHS) of the National ...
Breaking Biology News(10 mins):Frontiers news briefs: May 23 2Frontiers news briefs: May 23 3PNNL staff recognized for scientific accomplishments, moving technologies into the marketplace 2Emory, Georgia Tech receive first human exposome center grant in US 2Emory, Georgia Tech receive first human exposome center grant in US 3
... of the department of genetics, development and cell biology at ... work will be published in the Oct. 12 issue of ... of the green algae chlamydomonas, a single-celled alga that has ... of uses that come out of this, including basic bio-medical ...
... for researching and developing new biopharmaceuticals would be ... of dollars annually, according to a new economic ... and Technology (NIST). , Prepared by RTI ... over the last two decades emphasis in new ...
... project, 115 scientists from around the world report in the ... of data on a tiny green alga called Chlamydomonas, with ... organism that grows in soil and ponds, has approximately 15,000 ... of its genome. Several years ago, they knew less than ...
Cached Biology News:Biopharmaceutical infrastructure key to lower drug development costs 2Study involving more than 100 scientists provides new insights on green algae 2Study involving more than 100 scientists provides new insights on green algae 3Study involving more than 100 scientists provides new insights on green algae 4
... CRE-bla HEK 293T cell line contains the ... cAMP response element (CRE). To obtain the ... into HEK 293T cells by lentivirus. Subsequent ... responsive to forskolin stimulation (Figure 1). The ...
activating transcription factor 4 (tax-responsive enhancer element B67),...
Reactivity: Human...
... of BAC (100+kb), fosmid (40kb), and large ... than any othe BAC vector. On command ... and easy recombinant DNA purification. Available with ... VC) vectors for the highest insert stability. ...
Biology Products: