IMAP assays to be developed. In those assays
where the ATP concentration required is
greater than 30 M or substrate sequences
contain a number of acidic residues, the
IMAP Progressive Binding System provides
maximum flexibility, while the IMAP
Tween reaction buffer minimizes inhibitor
aggregation and sequestration. The IMAP
Substrate Finder kits enable researchers to
efficiently determine the best substrates to use
for profiling and speed assay development.
By virtue of the availability of both 5FAMand
5TAMRA-labeled substrates and the
relatively high (100 nM) fluor concentration
employed, the IMAP system can be designed
for maximum resistance to interferences from
your specific drug candidates.
For more information about these and other
IMAP products, go to the Reagents area
of the Molecular Devices web site at www.
KINASE INHIBITION REFERENCES
1. F. Meggio, D.A. Donella, M. Ruzzene, A.M.
Brunati, L. Cesaro, B. Guerra, T. Meyer, H.
Mett, D. Fabbro, P. Furet, et al. Different
susceptibility of protein kinases to staurosporine
inhibition. Kinetic studies and molecular bases
for the resistance of protein kinase CK2.
Eur J Biochem 1995 234(1):31722.
2. C.M. Seynaeve, M.G. Kazanietz, P.M.
Blumberg, E.A. Sausville, P.J. Worland.
Differential inhibition of protein kinase C
isozymes by UCN-01, a staurosporine analogue.
Mol Pharmacol 1994 45(6):120714.
3. S. Leclerc, M. Garnier, R. Hoessel, D. Marko,
J.A. Bibb, G.L. Snyder, P. Greengard, J. Biernat,
Y.Z. Wu, E.M. Mandelkow, G. Eisenbrand,
L. Meijer. IndirubinsPage: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Related biology technology :1
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