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Setting up a kinase profiler with IMAP

to 1 mM) of ATP concentrations.


THE PANEL OF KINASES
Because IMAP is a generic assay system, virtually any protein kinase can be tested. This allows the researcher to set up all profiling assays in the same system and to freely choose which kinases to screen. The only limitation is the availability of active purified kinase. For the purposes of this Application Note, a proposed Profiler Kinase Panel was constructed by choosing enzymes from all families of the kinome (Figure 1), as shown in Table 1.


Table 1. Proposed Kinases for a Profiler with IMAP Enzyme Validated Substrates Available From Molecular Devices Page 1 AMPK untested 4 2 Aurora B untested 5 3 CDK5/p25 5FAM-GGGPATPKKAKKL-COOH 8 4 CK1 5FAM-HAAIGDDDDAYSITA-NH2 8 5 FAK untested 8 6 IRAK4 untested 11 7 JNK2a2 untested 14 8 NEK2 5FAM-IRRLSTRRR-COOH 15 9 PAK4 untested 17 10 PKC'"/>

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