0.05 - 5FAM- ERMRPRKRQGSVRRRV-NH₂ RP7048 100/0/1:400 7 JNK2a2 0.04 - LVEPLTPSGEAPNQK-5FAM-COOH R7129 60/40/1:1200 8 NEK2 0.01 - 5FAM-GTFRSSIRRLSTRRR-COOH RP7140 100/0/1:400 9 PAK4 0.09 - 5FAM-KKRPQRRYSNVF-COOH RP7008 100/0/1:400 10 PKCx 0.003 - 5FAM-ERMRPRKRQGSVRRRV-NH₂ RP7048 100/0/1:400 11 Ros 0.05 2 mM MnCl₂ 5FAM-KKKSPGEYVNIEFG-NH₂ RP7069 75/25/1:600 12 TIE2 0.05 2 mM MnCl₂ 5FAM-EFPIYDFLPAKKK-NH₂ R7188 60/40/1:1200

Note to Table 4: Table 4 shows the details of the Profiling Screen. There are 12 kinases assayed utilizing 10 different substrates. The AMPK requires AMP in the reaction for full activation, and the three tyrosine kinase reactions contain MnCl₂. Binding Solutions are listed as % Buffer A/ % Buffer B/ dilution of BR. The EC₅₀s determined for each kinase are listed. For those with an asterisk, the SF assays and/or titration curves are
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