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Setting up a kinase profiler with IMAP

0.05 - 5FAM- ERMRPRKRQGSVRRRV-NH2 RP7048 100/0/1:400 7 JNK2a2 0.04 - LVEPLTPSGEAPNQK-5FAM-COOH R7129 60/40/1:1200 8 NEK2 0.01 - 5FAM-GTFRSSIRRLSTRRR-COOH RP7140 100/0/1:400 9 PAK4 0.09 - 5FAM-KKRPQRRYSNVF-COOH RP7008 100/0/1:400 10 PKCx 0.003 - 5FAM-ERMRPRKRQGSVRRRV-NH2 RP7048 100/0/1:400 11 Ros 0.05 2 mM MnCl2 5FAM-KKKSPGEYVNIEFG-NH2 RP7069 75/25/1:600 12 TIE2 0.05 2 mM MnCl2 5FAM-EFPIYDFLPAKKK-NH2 R7188 60/40/1:1200

Note to Table 4: Table 4 shows the details of the Profiling Screen. There are 12 kinases assayed utilizing 10 different substrates. The AMPK requires AMP in the reaction for full activation, and the three tyrosine kinase reactions contain MnCl2. Binding Solutions are listed as % Buffer A/ % Buffer B/ dilution of BR. The EC50s determined for each kinase are listed. For those with an asterisk, the SF assays and/or titration curves are
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