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Setting up a kinase profiler with IMAP

signal. To simplify the protocol, the entire Profiler assay was incubated overnight with Binding Solution. While there is an optimal IMAP Binding Solution for every substrate sequence, it is possible to optimize Binding Solution compositions to accommodate a variety of substrate sequences with good results. This feature can aid in automation of Profiler Systems with IMAP and simplify assay set-up.

Table 4. Setup of Profiling Screen Enzyme EC50 (U/mL) Special Reaction Requirements Substrate Sequence Substrate Part Number (8000 Test Points) Binding Solution (%A/%B/BR Dilution) 1 AMPK 0.01 50 M AMP 5FAM-LKKLRRRLSDANF-NH2 RP7005 100/0/1:400 2 Aurora B 0.04 - 5FAM-LKKLRRRLSDANF-NH2 RP7005 100/0/1:400 3 CDK5/p25 0.05* - 5FAM-GGGPATPKKAKKL-COOH R7252 100/0/1:400 4 CK1 0.008* - 5FAM-HAAIGDDDDAYSITA-NH2 R7311 40/60/1:2500 5 FAK 0.003 2 mM MnCl2 5FAM-GEEIYGEFD-NH2 RP7060 10/90/1:1500 6 IRAK4
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