Navigation Links
Setting up a kinase profiler with IMAP

round and maximize the FP signal.

The three substrates that provided the greatest DmP in the assay shown in Figure 17B were then chosen for a subsequent TIE2 titration assay, presented in Figure 18. All of these substrates are part of the MDC peptide inventory. In the SF Tyr plate, their well IDs are as follows: C13D14 (Blk/Lyntide), C7D8 (CSKtide) and K17L18 (PDGFRtide); their sequences and Binding Solutions are also given in Table 3. The TIE2 substrate that ranked the best in terms of DmP and EC50 was the FAM-Blk/Lyntide, so this peptide was chosen for the profiling assay.


SETTING UP THE PROFILER SYSTEM
Now that the specifics of each enzyme assay have been determined, the Profiler can be assembled. All of the Substrate Finder peptides are available from Molecular Devices. (Contact Molecular Devices Customer Service or go to www.moleculardevices.com for details.) Table 4 shows the updated Profiler from Table 1, including any special reaction requirements, the substrate to use and the optimized binding solution for each substrate.

For kinase selectivity profiling, the screen can be set up in a wide variety of formats. It can be designed
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Related biology technology :

1. Setting up the laboratory to avoid contamination
2. Reduce Errors and Save Time When Setting Up PCRs
3. Setting up Successful siRNA Library Screens
4. LiquiChip Kits for bead-based cytokine and kinase assays
5. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
6. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Setting kinase profiler with IMAP

(Date:7/24/2014)... 24, 2014 SRI International has been awarded ... Institute of Allergy and Infectious Diseases (NIAID), part of ... of potential therapies for HIV infection and AIDS. The ... and AIDS and the complications and opportunistic infections associated ... sexual transmission of HIV. According to ...
(Date:7/24/2014)... 2014 Deep Research Report on Global ... and in-depth research report on Potassium Sulphate ... information, including its definition, classification, application, and industry ... the international market analysis, including China’s domestic market ... environment & economic situation analysis, the influence of ...
(Date:7/24/2014)... July 24, 2014 1000 balances – ... input on customers’ weighing needs and expertly narrows down ... of balances, scales or even terminals. , First, ... smallest weight (or select readability instead) and define the ... additional selection criteria narrows down the list to models ...
(Date:7/24/2014)... July 24, 2014  Spherix Incorporated (SPEX) -- an ... monetization of intellectual property, today announced that the Company ... June 30, 2014 on Monday, August 18, 2014 for ... a.m. ET on Monday, August 18, 2014 to discuss ... the company,s CEO, Anthony Hayes . ...
Breaking Biology Technology:National Institute of Allergy and Infectious Diseases Awards SRI International Contract to Study New Therapies for HIV and AIDS 2National Institute of Allergy and Infectious Diseases Awards SRI International Contract to Study New Therapies for HIV and AIDS 3Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 2Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 3Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 4Spherix Schedules Shareholder Update Call 2
... Leum Software Solutions, Inc. has announced an exclusive ... Ascend retail management software package. Trek and Leum Software intend ... with additional tools and features to help them manage their ... ways to make our dealers more successful. In the future, ...
... but incorporate software-based components (such as microprocessors or microcontrollers) ... importance of protecting that software. The software may not ... of the product, but a feature nonetheless and a ... ,For example, many vehicles, such as motorcycles, trucks and ...
... - To complement the two- and four-port Ethernet cards ... has released software for Apple G4 and G4 servers ... with more bandwidth. , ,The company is the first to ... Mac platforms. In March, it expanded from the G5 to ...
Cached Biology Technology:Beyond the screws and gears: Protecting your products by protecting your software 2Small Tree boosts bandwidth for Apple G4 and G5 servers 2
(Date:7/25/2014)... The routine use of a molecular testing panel ... performing the correct initial surgery for patients with ... University of Pittsburgh Cancer Institute (UPCI), partner with ... Multidisciplinary Thyroid Center and other diagnostic testing agencies, ... initial surgery by 30 percent, according to the ...
(Date:7/24/2014)... Scientists at The New York Stem Cell Foundation ... creating a viable cell replacement therapy for multiple ... For the first time, NYSCF scientists generated induced ... of patients with primary progressive multiple sclerosis and ... these stem cells into becoming oligodendrocytes, the myelin-forming ...
(Date:7/24/2014)... 24, 2014 /PRNewswire-iReach/ -- The Geneva Healthcare Suite, an ... has cut emergency room wait times by an average ... Medical Center, according to a recent study published in ... are using the suite to access data from incoming ... "Using Geneva,s technology platform we have been ...
Breaking Biology News(10 mins):Test increases odds of correct surgery for thyroid cancer patients 2Test increases odds of correct surgery for thyroid cancer patients 3NYSCF scientists one step closer to cell therapy for multiple sclerosis patients 2UC San Diego Medical Center Cuts Average ER Wait Time by 92 Minutes using the Geneva Healthcare Suite 2UC San Diego Medical Center Cuts Average ER Wait Time by 92 Minutes using the Geneva Healthcare Suite 3
... heat or electricity, has to be used right away. It ... can be rather complicated. Creating solar cells capable of producing ... to say fuel, is therefore the future challenge of solar ... on a catalyst that imitates and improves what nature has ...
... 2013 A new study from the Department of Nutrition, ... beverages (SSBs) are primarily responsible for higher caloric intakes of ... not (on a given day). In addition, SSB consumption is ... are published in the American Journal of Preventive Medicine ...
... chemicals and a stagnant atmosphere were behind what became ... new NASA study finds. Even when both poles ... the Arctic,s ozone depletion tends to be milder and ... key ingredients needed for ozone-destroying chemical reactions chlorine from ...
Cached Biology News:Catalysts that produce 'green' fuel 2Kid's consumption of sugared beverages linked to higher caloric intake of food 2NASA pinpoints causes of 2011 Arctic ozone hole 2NASA pinpoints causes of 2011 Arctic ozone hole 3
... GCpro Taq DNA Polymerase is a ... advantages for all PCR applications. GCpro Taq ... clone expressed in E. coli, containing the ... The enzyme includes a highly processive 5'-3' ...
Ps-Aeruginosa (IgG) -Ab EIA Sample Size: 100 l...
...
Automated -80° C sample library for microplates and cryotube racks in SBS format; for integration into automated processes....
Biology Products: