Navigation Links
Setting up a kinase profiler with IMAP

round and maximize the FP signal.

The three substrates that provided the greatest DmP in the assay shown in Figure 17B were then chosen for a subsequent TIE2 titration assay, presented in Figure 18. All of these substrates are part of the MDC peptide inventory. In the SF Tyr plate, their well IDs are as follows: C13D14 (Blk/Lyntide), C7D8 (CSKtide) and K17L18 (PDGFRtide); their sequences and Binding Solutions are also given in Table 3. The TIE2 substrate that ranked the best in terms of DmP and EC50 was the FAM-Blk/Lyntide, so this peptide was chosen for the profiling assay.


SETTING UP THE PROFILER SYSTEM
Now that the specifics of each enzyme assay have been determined, the Profiler can be assembled. All of the Substrate Finder peptides are available from Molecular Devices. (Contact Molecular Devices Customer Service or go to www.moleculardevices.com for details.) Table 4 shows the updated Profiler from Table 1, including any special reaction requirements, the substrate to use and the optimized binding solution for each substrate.

For kinase selectivity profiling, the screen can be set up in a wide variety of formats. It can be designed
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Related biology technology :

1. Setting up the laboratory to avoid contamination
2. Reduce Errors and Save Time When Setting Up PCRs
3. Setting up Successful siRNA Library Screens
4. LiquiChip Kits for bead-based cytokine and kinase assays
5. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
6. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Setting kinase profiler with IMAP

(Date:8/29/2014)... Albany, New York (PRWEB) August 29, 2014 ... be valued at $5.6 billion in 2019. It ... 18.1% from 2013 to 2019, and was valued ... research report published by Transparency Market Research. , ... please visit: http://www.transparencymarketresearch.com/companion-diagnostics-market.html . , The research ...
(Date:8/29/2014)... PA (PRWEB) August 29, 2014 Pittcon ... Hub MacDonald, and colleague Koichiro Matsuda, Horiba Scientific, have ... on September 5, 2014. JASIS , Asia’s largest ... 3-5, 2014, in Makuhari Messe, Japan. , The ... Tools for Bioanalysis from Single Molecules to Single Cells” ...
(Date:8/29/2014)... Local veterinarian, Dr. Keith Clement, and his ... to participate in an investigational study of donor stem ... of this study is to determine if a single ... arthritically affected joints can help reduce pain and inflammation ... current investigational study must be older than nine months, ...
(Date:8/29/2014)... 29, 2014 Due to a misstatement posted ... the Adult Stem Cell Technology Center, LLC ( ASCTC ) ... cell therapy research that might benefit ALS patients. Because ... new stem cell technologies and therapeutic applications, FRC listed ASCTC ... Challenge donors who wish not to support research that ...
Breaking Biology Technology:Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 2Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 3Fifth Annual U.S. Symposium at JASIS Organized by Pittcon’s Program Chairman Hub MacDonald 2Burnt Hills Veterinary Hospital Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2The Adult Stem Cell Technology Center, LLC Declines ALS Ice Bucket Challenge Donors Wishing to Support Ethical Research for New Stem Cell Therapies 2The Adult Stem Cell Technology Center, LLC Declines ALS Ice Bucket Challenge Donors Wishing to Support Ethical Research for New Stem Cell Therapies 3
... Pharma ... SOMERVILLE, Mass., May 20 Spotfire, a,division of TIBCO ... Senior Director Life Sciences Analytics, will present,Applying Next Generation Business ... Conference being held May 20 & 21 in Basel,Switzerland. Mr. ...
... a considerable impact on many aspects of modern ... the human frontier, robotics is also vigorously engaged ... Interacting, exploring, and working with humans, the new ... The prospect of practical robots among humans follows ...
... Forward-Looking IVUS technology platform expected to enable, Volcano to ... ... therapeutic devices, SAN DIEGO, May 19 Volcano Corporation,(Nasdaq: ... and Optical Coherence Tomography (OCT) products designed,to enhance the diagnosis and ...
Cached Biology Technology:TIBCO Spotfire Puts the Spotlight on Screening at InfoTech for Pharma and Biotech 2TIBCO Spotfire Puts the Spotlight on Screening at InfoTech for Pharma and Biotech 3Robots moving closer to humans 2Volcano Announces Acquisition of Novelis, Inc. 2Volcano Announces Acquisition of Novelis, Inc. 3Volcano Announces Acquisition of Novelis, Inc. 4Volcano Announces Acquisition of Novelis, Inc. 5Volcano Announces Acquisition of Novelis, Inc. 6Volcano Announces Acquisition of Novelis, Inc. 7
(Date:8/29/2014)... release is available in German . ... players in the natural nitrogen cycle on Earth and ... bacteria were thought to depend on nitrite as their ... by Holger Daims, a microbiologist at the University of ... hydrogen as an alternative source of energy. The oxidation ...
(Date:8/29/2014)... MO. - In a cell,s nucleus, chromosomal DNA ... histones, an amalgam biologists call chromatin. Until about ... nuclear "sidekick," the mere packing material around which ... biologists have developed a greater appreciation for how ... investigators from multiple research institutions studying the sequence ...
(Date:8/28/2014)... TX Invasives is now available from the Lady Bird ... Austin for identifying harmful non-native plant, insect and other ... United States cause about $137 billion in economic and ... waterways, kill native plants and more. , "By having ... where invasive species occur, we,re more likely to catch ...
Breaking Biology News(10 mins):Hydrogen powers important nitrogen-transforming bacteria 2Hydrogen powers important nitrogen-transforming bacteria 3'K-to-M' histone mutations: How repressing the repressors may drive tissue-specific cancers 2'K-to-M' histone mutations: How repressing the repressors may drive tissue-specific cancers 3Mobile app makes ID of harmful plants, insects in Texas a snap 2
... The National Institutes of Health has awarded Dr. Daniel Geschwind, ... a five-year, $10 million grant to continue his research on ... his investigations to include the genetics of autism in African ... fund collaborative work by Geschwind and experts at other autism ...
... A University of Alberta researcher has identified some of ... long distances. , Working together with an international ... examined unusual claw marks left on a river bottom ... major travel-way for dinosaurs., Alongside easily identified fossilized footprints ...
... N.Y. As the body ages, the physical effects ... becomes harder. But there are also less visible processes ... at work, which may contribute to age-related brain defects. ... the journal Nature Neuroscience CSHL Associate Professor ...
Cached Biology News:Autism in black and white: NIH grant helps scientist study disorder in African Americans 2CSHL neuroscientists show 'jumping genes' may contribute to aging-related brain defects 2CSHL neuroscientists show 'jumping genes' may contribute to aging-related brain defects 3
Recognizes the VZV nucleocapsid by indirect immunofluorescence and immunoprecipitation. Shows cross-reactivity to HSV....
Rabbit polyclonal to Orexin Prepro ( Abpromise for all tested applications). entrezGeneID: 3060 SwissProtID: O43612...
Adenylate kinase isoenzyme 6...
AK6 Antibody...
Biology Products: