Navigation Links
Setting up a kinase profiler with IMAP

F Ser/Thr 2 kit has a protocol and set of peptide substrates distinct from the SF Ser/Thr 1 and SF Tyr kits. These IRAK 4 results are shown in Figure 8, Panel B. The four peptides on the IRAK SF Ser/Thr 2 plate that provided the greatest DmP were ID C3D4, A9B10 and G9H10. A9 B10 is the SF Ser/Thr 2 ID for the same FAM-PKCe pseudosubstrate-derived peptide discussed above, which underscores the reproducibility among IMAP SF kits. There are nine substrates common to both the SF Ser/Thr 1 and SF Ser/Thr 2 kits because they were described as substrates for kinases from both areas of the kinome. (See Figure 1.) Figure 9 shows the corresponding IRAK 4 dilution curves with the SF Ser/Thr 1 and SF Ser/Thr 2 substrates. The FAM-PKCe-derived peptide, of sequence 5FAM-ERMRPRKRQGSVRRRVNH2, had the lowest EC50 of all peptides tested. This peptide was therefore chosen to be the IRAK 4 profiling substrate.

JNKa2 is another kinase that does not yet have available substrates optimized for IMAP. For initial assay development, usable substrates for JNK2a2 were determined with the SF Ser/Thr 2 kit. (See Figure 10.)

As only 0.17 U/mL JNK2a2 was assayed on the SF Ser/Thr 2 plate, few peptides were hits in this assay. The EGFR-derived peptide (well ID E17-F18), an IMAP-validated substrate already in MDC's inventory, provided the sole signal greater than 100 mP. To confirm that this peptide would i
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Related biology technology :

1. Setting up the laboratory to avoid contamination
2. Reduce Errors and Save Time When Setting Up PCRs
3. Setting up Successful siRNA Library Screens
4. LiquiChip Kits for bead-based cytokine and kinase assays
5. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
6. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Setting kinase profiler with IMAP

(Date:1/15/2014)... January 15, 2014 This webinar ... on nonclinical and clinical safety assessment in biosimilars. ... guide a path for biosimilar drug development, however ... of biosimilarity with regards to quality, safety and ... of biosimilar drug development and registration, development strategies ...
(Date:1/15/2014)... NC (PRWEB) January 15, 2014 ... an enhancement to its Online Web Portal for Life ... now have the ability to specify the subject matter ... nearly 50 life science sub-domains. This will help reduce ... and scientific fields, and decrease the likelihood of error. ...
(Date:1/14/2014)... Doylestown, PA (PRWEB) January 14, 2014 ... – 11 p.m. , Location: Warrington Country Club, 1360 ... Hepatitis B Foundation, the only national nonprofit organization solely ... improving the quality of life for those affected worldwide, ... 11 at Warrington Country Club in Warrington, Pa. ...
(Date:1/14/2014)... York, NY (PRWEB) January 14, 2014 ... initiated coverage of Alliqua, Inc. (OTCQB: ALQA). Alliqua ... and marketing proprietary products to serve the wound ... , In late 2012, Alliqua was restructured ... launched the company’s new strategy to become a ...
Breaking Biology Technology:Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2DTS Improves Efficiency for Life Science Document Translations 2Hepatitis B Foundation to Host Annual Crystal Ball Gala 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3
... 2008,November 4th through 9th,in Philadelphia, Pennsylvania, MISGAV, ... is pleased to announce encouraging preliminary data for ... I/II clinical trial of Medgenics, EPODURE Biopump,for providing ... kidney,disease, is underway. The current trial is designed ...
... Sunesis,Pharmaceuticals, Inc. (Nasdaq: SNSS ), a biopharmaceutical company ... for,the treatment of solid and hematologic cancers, today announced ... conferences:, Rodman & Renshaw 10th Annual Healthcare Conference, ... Wednesday, November 12, 2008, 2:00 p.m. EST, ...
... JERSEY CITY, New Jersey, November 3, Rosetta Genomics ... development of microRNA-based diagnostics, announced today it will,present ... the Lab and Clinic in Cancer Medicine" Molecular ... take place,between November 3-6, at the ICC Jerusalem ...
Cached Biology Technology:Medgenics Announces Encouraging Preliminary Data For Phase I/II Clinical Trial of EPODURE In Renal Anemia 2Medgenics Announces Encouraging Preliminary Data For Phase I/II Clinical Trial of EPODURE In Renal Anemia 3Medgenics Announces Encouraging Preliminary Data For Phase I/II Clinical Trial of EPODURE In Renal Anemia 4Medgenics Announces Encouraging Preliminary Data For Phase I/II Clinical Trial of EPODURE In Renal Anemia 5Sunesis to Present at Upcoming Conferences 2Rosetta Genomics to Present at the American Association of Cancer Research (AACR) Centennial Conference 2Rosetta Genomics to Present at the American Association of Cancer Research (AACR) Centennial Conference 3
(Date:4/15/2014)... study the deadliest infectious diseases recognized the contributions of ... the University of Texas Medical Branch, with a Lifetime ... Filoviruses. The filoviruses include Ebola and Marburg viruses that ... infected. The current outbreak of Ebola virus raging in ... far. , "This award represents the culmination of ...
(Date:4/15/2014)... University dental researchers have found a less invasive ... the mouth to study how the mouth,s natural ... isolating some specialized immune cells (white blood cells ... diseases in the mouthor reject foreign tissues, such ... more about treating and preventing such health issues ...
(Date:4/15/2014)... 2014) The American Association of Anatomists (AAA) is ... awards will be presented during the Closing Awards Ceremony ... Tuesday, April 29th at 7:30 p.m. during AAA,s 2014 ... The 2014 award winners are: , Henry ... the Icahn School of Medicine at Mount Sinai, Past-President ...
Breaking Biology News(10 mins):International research group recognizes UTMB experts 2International research group recognizes UTMB experts 3New method isolates immune cells for researchers to study how they ward off oral diseases 2American Association of Anatomists 2014 award winners 2
... the meaning of a rare mineral that can be used to ... and others better understand what we,re probably in for over the ... the heat and, ultimately, to change life as we know ... place in the next hundred years or so, and that many ...
... colloquium to examine what lies ahead in evolutionary studies ... and students will discuss new tools to predict future ... of ecology, paleontology, phylogenetics, and biodiversity informatics dealing with ... series, the event will take place at the Arnold ...
... (ST. LOUIS): The Missouri Botanical Garden Press (MBG ... species of vascular plants for the Southern Cone region, ... Plantas Vasculares del Cono Sur (Catalogue of the Plants ... the plants of Argentina, southern Brazil, Chile, Paraguay and ...
Cached Biology News:Foretelling a major meltdown 2Foretelling a major meltdown 3Missouri Botanical Garden publishes first catalogue of plants of the Southern Cone 2
hnRNP U (H-94)...
Rabbit polyclonal antibody to Neuromedin U Receptor 1 (GPR66)...
... GELase™ Agarose Gel-Digesting Preparation contains a ... for simple, quantitative recovery of intact DNA ... agarose gels following electrophoresis in TAE, TBE, ... be digested directly in the TAE, TBE, ...
... in-house demand is causing shortages in your ... This can cause unwanted distractions, problems and ... royalties for your hard work and endeavour! ... build it up,and market it internationally. We ...
Biology Products: