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Setting up Successful siRNA Library Screens


siRNA Libraries Tie Genes to Cell Functions

Ambion has developed human siRNA libraries that target important gene classes including kinases, ion channels, receptor classes, molecular motors, and many others (Figure 1). Applying these siRNA libraries to cell culture screens reveals human genes that are critical for cellular functions. The diversity of cell functions that can be characterized using siRNA libraries is limited only by the range of phenotypic assays that can be developed. With siRNA libraries, you will be able to identify critical human genes faster and easier than ever before.

Figure 1. Ambion siRNA Libraries. [Additional Libraries and Ordering Information]


Scientists at Ambion have already used several Silencer siRNA libraries in quantitative assays to identify genes involved in apoptosis, cell proliferation, TRAIL induction, and p53 activation. Below we provide a general overview of an effective screening process. See Recommendations for Successful siRNA Library Screens, for tips for successful screening experiments.


Overview of the Screening Process

(1) Develop a quantitative assay to monitor cellular process being studied.

Assays that measure the intensity of a cellular phenotype range from microscopic assays that monitor
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Related biology technology :

1. Setting up the laboratory to avoid contamination
2. Reduce Errors and Save Time When Setting Up PCRs
3. Setting up a kinase profiler with IMAP
4. Keys to Successful Densitometry
5. Successful PCR amplification and subcloning of a GC-rich DNA fragment
6. General Considerations for Successful Transfection Experiments
7. Designing a Successful qRT-PCR Experiment
8. Precursor miRNAs for Successful miRNA Functional Studies
9. Recommendations for Successful siRNA Library Screens
10. Successful stabilization of gene expression profiles
11. Custom and library siRNA for efficient gene silencing
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