Navigation Links
Setting up Successful siRNA Library Screens


siRNA Libraries Tie Genes to Cell Functions

Ambion has developed human siRNA libraries that target important gene classes including kinases, ion channels, receptor classes, molecular motors, and many others (Figure 1). Applying these siRNA libraries to cell culture screens reveals human genes that are critical for cellular functions. The diversity of cell functions that can be characterized using siRNA libraries is limited only by the range of phenotypic assays that can be developed. With siRNA libraries, you will be able to identify critical human genes faster and easier than ever before.

Figure 1. Ambion siRNA Libraries. [Additional Libraries and Ordering Information]


Scientists at Ambion have already used several Silencer siRNA libraries in quantitative assays to identify genes involved in apoptosis, cell proliferation, TRAIL induction, and p53 activation. Below we provide a general overview of an effective screening process. See Recommendations for Successful siRNA Library Screens, for tips for successful screening experiments.


Overview of the Screening Process

(1) Develop a quantitative assay to monitor cellular process being studied.

Assays that measure the intensity of a cellular phenotype range from microscopic assays that monitor
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Setting up the laboratory to avoid contamination
2. Reduce Errors and Save Time When Setting Up PCRs
3. Setting up a kinase profiler with IMAP
4. Keys to Successful Densitometry
5. Successful PCR amplification and subcloning of a GC-rich DNA fragment
6. General Considerations for Successful Transfection Experiments
7. Designing a Successful qRT-PCR Experiment
8. Precursor miRNAs for Successful miRNA Functional Studies
9. Recommendations for Successful siRNA Library Screens
10. Successful stabilization of gene expression profiles
11. Custom and library siRNA for efficient gene silencing
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Setting Successful siRNA Library Screens

(Date:1/15/2014)... 2014 Look inside the new Preferred ... the lab, from fluid handling to instruments to supplies. ... when you order. , Preferred Solutions features a ... the L/S® model for precise flow control and dispensing ...
(Date:1/15/2014)... Canada (PRWEB) January 15, 2014 This ... on nonclinical and clinical safety assessment in biosimilars. , ... path for biosimilar drug development, however the complex nature ... to quality, safety and efficacy extremely challenging. Based on ...
(Date:1/15/2014)... Rochester, NY (PRWEB) January 15, 2014 The ... cause a major disease. One of these latent viruses is ... is rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic ... the theory, a study found that RA patients have high ...
(Date:1/15/2014)... Toronto, ON (PRWEB) January 15, 2014 ... an experimental new therapy for the treatment of tinnitus. ... unique tinnitus frequency, and over a period of weeks to ... Notched Sound Therapy in two forms: Notched Music and Notched ...
Breaking Biology Technology:Cole-Parmer Begins 2014 with the Release of Preferred Solutions 2Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3
... Inc. (Amex: ADL ), today announced the promotion ... (COO) and CFO. The Company is also,expanding its management ... of International Marketing and Sales and Mr. Raymond Gatchalian ... and CFO, Mr. Ariura will lead the day-to-day operations ...
... SNDY) is pleased to announce that the Company has ... St. Mary,s,Hospital in Waterbury, CT. St. Mary,s has been ... it has chosen to continue using the,Company,s surgical products., ... in the history of Greater,Waterbury, providing nearly a century ...
... CHICAGO, Nov. 6 Advanced Life Sciences,Holdings, Inc. ... in the,discovery, development and commercialization of novel drugs ... diseases, today,announced its financial results for the third ... ), The net loss available to common ...
Cached Biology Technology:AMDL, Inc. Promotes CFO Akio Ariura to Chief Operating Officer and Expands Management Team 2AMDL, Inc. Promotes CFO Akio Ariura to Chief Operating Officer and Expands Management Team 3AMDL, Inc. Promotes CFO Akio Ariura to Chief Operating Officer and Expands Management Team 4Solos Endoscopy, Inc. Receives Purchase Order from Connecticut Hospital 2Advanced Life Sciences Announces Third Quarter 2008 Financial Results 2Advanced Life Sciences Announces Third Quarter 2008 Financial Results 3Advanced Life Sciences Announces Third Quarter 2008 Financial Results 4Advanced Life Sciences Announces Third Quarter 2008 Financial Results 5Advanced Life Sciences Announces Third Quarter 2008 Financial Results 6Advanced Life Sciences Announces Third Quarter 2008 Financial Results 7Advanced Life Sciences Announces Third Quarter 2008 Financial Results 8Advanced Life Sciences Announces Third Quarter 2008 Financial Results 9Advanced Life Sciences Announces Third Quarter 2008 Financial Results 10Advanced Life Sciences Announces Third Quarter 2008 Financial Results 11
(Date:4/17/2014)... deforestation and fragmentation of forests in the Amazon ... forests, contributing to rapid and widespread forest loss ... researchers., The findings show that forests in the ... droughts coupled with forest fires lead to large-scale ... Jennifer Balch, assistant professor of geography, Penn State. ...
(Date:4/17/2014)... new early-warning signs of the potential loss of sight ... for the diagnosis and treatment of diabetic retinopathy, potentially ... "We had not expected to see such striking changes ... Elsner, professor and associate dean in the IU School ... set out to study the early signs, in volunteer ...
(Date:4/17/2014)... the Australian National University (ANU), have developed a ... temperature variability over the past 5.3 million years., ... climate surrounding ice ages over the past two ... between carbon dioxide levels, global temperatures and sea ... Southampton (UoS) and the National Oceanography Centre (NOC) ...
Breaking Biology News(10 mins):Drought and fire in the Amazon lead to sharp increases in forest tree mortality 2Drought and fire in the Amazon lead to sharp increases in forest tree mortality 3New technique detects microscopic diabetes-related eye damage 2New technique detects microscopic diabetes-related eye damage 3Ancient sea-levels give new clues on ice ages 2
... could also be giving us bad breath, according to research ... Medical Microbiology . For the first time, scientists have found ... who are not showing signs of stomach disease. The ... some of which can cause disease. Helicobacter pylori ...
... set of proteins known as cannabinoid receptors, which ... body also has chemicals known as endocannabinoids that ... (AEA) and 2-arachidonoylglycerol (2-AG). These natural components of ... efforts to develop new treatments not only for ...
... Buenos Aires, Argentina Current research suggests that melatonin ... disease. The related report by Sande et al., "Therapeutic ... December issue of The American Journal of Pathology ... pain in their eyes, and their vision rapidly deteriorates. ...
Cached Biology News:Stomach ulcer bug causes bad breath 2Scripps research team defines new painkilling chemical pathway 2Melatonin may save eyesight in inflammatory disease 2
Chicken polyclonal to ADI1 ( Abpromise for all tested applications). Antigen: Full length native protein (purified), corresponding to amino acids 1-179 of Human ADI1. Entrez GeneID: 55256 ...
anti-JNK2 Immunogen: Recombinant human JNK2 protein Accession Number: NM_002752 Quality Assurance: Routinely evaluated by immunoblot. Molecular Weight: 46 and/or 54 kDa Available ...
Gloves are 9.25 long, latex, powder free, smooth and ambidextrous....
Request Info...
Biology Products: