( cision is 3.9% significantlylower t...)Serial Dilution with the Bravo Liquid Handling Platform

cision is 3.9%, significantly lower than 15% at a height of 0.1mm. Following the same trend as previously observed, accuracy tracks with precision, so the higher mix height also improves our accuracy ratio to 1.95 (Table 2). This trend is observed because the higher dispense height at this dispense speed ensures that more of the sample is circulated by the mix cycle: in a mix far from the bottom of the well, dispensed liquid is forced closer to the bottom while dispensing, and aspirated liquid is pulled from the top of the well. If the mix occurs closer to the bottom of the plate, the dispensed liquid is pulled back into the tip during the aspiration.

Mix Liquid Class Setting
The next parameter explored was the mix liquid class settings. Each liquid class has a set of velocities and accelerations for the aspirate and dispense. This experiment was to determine if increasing the aspirate and dispense speeds of the mix would result in improved serial dilution results.

The original liquid class speed for the mix was 100 μL/s velocity and 500 μL/s² acceleration. Based on the average precision of the data (Table 3), mixing became more effective as the mix speed is increased. Accuracy is also improved as the mix speed increases, because of consistency of the mixes across the plate. Under these conditions, a mix liquid class setting of a velocity 300μL/s and an acceleration 1000μL/s² was utilized. There was no appreciable difference in time or performance by increasing the velocity from 300 μL/s to 500 μL/s.

Dynamic Tip Retraction/Extension
To further explore improving precision and accuracy, dynamic tip retraction/extension was used on the mix parameter. Utilizing this particular parameter in the mix cycle, the tip moves into the we
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