Navigation Links
Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

Serial Analysis of Gene Expression (SAGE) is a sequence-based approach to the identification of differentially expressed genes by comparative analyses. SAGE is a powerful tool, one of the more comprehensive methods available for detailed analysis of large numbers of cellular transcripts, leading to a profile of the expressed genes.1 SAGE provides an accurate quantitative analysis of the relative levels of genes expressed in a specimen. The ability to count many thousands of genes allows the detection of genes that are expressed at very low levels in a high-throughput manner. A three-step molecular approach, the SAGE process allows simultaneous analyses of sequences derived from various cell populations or tissues.2

Introduction

In 1995, Victor Velculescu first described SAGE, a pioneering approach to gene expression analysis that enabled a detailed characterization of expression patterns without prior knowledge of previously isolated genes. The method was originally developed to investigate genes that might be differentially expressed in colon cancer.4

Cancer research remains the focus of the vast majority of SAGE studies. The identification of novel markers for cancer progression and prognosis as in the case of uterine cancer, in which early stage I detection can be difficultis critical for successful treatment. The SAGE method can identify potential targets for therapeutic intervention and find key biochemical pathways. This application is well suited for gene expression analysis of any tissue from any organism.

SAGE is based on the principle that once isolated from a defined position within a transcripta sequence tag of 9 or 10 base pairs (bp) provides sufficient information to identify a transcript. These tags can be serially analyzed by ligating or concatenating them to form longer chains. After they are sequenced, software
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Serial Dilution with the Bravo Liquid Handling Platform
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Epitope-Tagging Vectors for Functional Analysis in Yeast
4. Map and Link Human Genetic Disorders with SSLP Analysis
5. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
11. Agilent 2100 Bioanalyzer Automated Analysis System
Post Your Comments:
(Date:2/27/2015)... 27, 2015  Pfenex Inc. (NYSE MKT: PFNX), a ... therapeutics, today announced that it will be presenting at ... March 2 nd at 3pm PST. Patrick Lucy, chief ... discussing the current state of the biosimilar industry landscape ... For more information on CALBIO 2015 and ...
(Date:2/27/2015)... South San Francisco, CA (PRWEB) February 27, 2015 ... it is joining forces on February 28 with patients ... globally to raise awareness for Rare Disease Day®. ... of rare diseases and calling attention to the special ... support community around them. , “Cytokinetics is proud to ...
(Date:2/27/2015)...   PureTech , a science and technology R&D ... and technologies in the healthcare sector, announced today the ... Member of the Board of Directors of Sanofi, to ... been a pleasure to know Chris for many years ... working together more closely now," said Dr. Robert ...
(Date:2/26/2015)... 2015 On behalf of biotech client ... Wilentz, Goldman & Spitzer, P.A. (Wilentz) secured a significant ... Inc. in excess of $10 million. The jury determined ... and that it improperly hired one or more of ... for judgment notwithstanding the verdict, a new trial, and ...
Breaking Biology Technology:Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 2Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 3Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 4Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 5Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 6Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 2Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 3Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 4Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 2Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 3
... website of the Nobel Prize shows a cat resting ... the excitement around graphene, which, at one atom thick, ... produced. A significant obstacle to realizing graphene,s potential ... a theoretical sleeping cat. For now, material scientists stitch ...
... N.Y. Rensselaer Polytechnic Institute Professor James Jian-Qiang ... and technical achievements toward the design and realization ... professor in the Department of Electrical, Computer, and ... the prestigious William D. Ashman Achievement Award for ...
... 2010 Amgen today announced the 11 recipients of the ... Amgen Foundation, the Awards recognize staff in the United States ... the communities where the company has a presence. The Amgen ... the winning staff members, applications. "At Amgen, we ...
Cached Biology Technology:Graphene's strength lies in its defects 2Rensselaer Polytechnic Institute professor James Lu garners award for research on 3-D computer chips 2Amgen Foundation Recognizes Outstanding Staff Volunteers and Contributes $55,000 to Their Favorite Local Nonprofits 2Amgen Foundation Recognizes Outstanding Staff Volunteers and Contributes $55,000 to Their Favorite Local Nonprofits 3Amgen Foundation Recognizes Outstanding Staff Volunteers and Contributes $55,000 to Their Favorite Local Nonprofits 4
(Date:2/25/2015)... , Feb. 25, 2015  ABC Financial ... billing in the Health and Fitness Industry, today ... as to MYiCLUBonline.  The latest upgrade includes advances ... inclusion of cardless check-in via Identity One fingerprint ... first time through interactive displays at the International ...
(Date:2/19/2015)... , Feb .19, 2015 Research and Markets ... addition of the "Military Electro-Optical / Infrared ... Technology, by Platform - Forecast to 2020" ... military electro-optical/infrared systems market is expected to reach ... of 7.71%. This report segments the ...
(Date:2/11/2015)... 2015 According to ... Market by Product (Cards and Readers, Biometrics, Controllers, Management ... Industrial, Healthcare, Education) and By Geography - Global Forecast and Analysis ... Access Control Market is expected to reach ... CAGR of 10.6% between 2014 and 2020. ...
Breaking Biology News(10 mins):ABC Financial Unveils New Software Enhancements 2ABC Financial Unveils New Software Enhancements 3Military Electro-Optical / Infrared Systems (Image Intensification, Laser, Infrared) Market - Forecast to 2020: Featuring Profiles of the Market Leaders on this $16 Billion market 2Military Electro-Optical / Infrared Systems (Image Intensification, Laser, Infrared) Market - Forecast to 2020: Featuring Profiles of the Market Leaders on this $16 Billion market 3Access Control Market Worth $10.4 Billion by 2020 2Access Control Market Worth $10.4 Billion by 2020 3Access Control Market Worth $10.4 Billion by 2020 4
... 20 years ago amid the stench and noise of a ... Island turns out to be the youngest example on the ... in California, according to University of California, Berkeley, paleontologists. ... that fall to the deep-ocean floor where, like an oasis ...
... N.H. The University of New Hampshire, in cooperation ... EcoLine, a landfill gas project that will pipe enriched ... to the Durham campus, UNH President Mark Huddleston announced. ... undertake a landfill gas initiative of this magnitude. ...
... dangerous wave of ecoterrorism has swept North America in the ... logging headquarters have all been the target of arson attacks, ... Earth Liberation Front past the $100 million mark. The ... it labeled environmental terrorism as the number one domestic terrorism ...
Cached Biology News:Ancient whale fall from California's Ao Nuevo Island one of youngest, most complete known 2Ancient whale fall from California's Ao Nuevo Island one of youngest, most complete known 3UNH becomes first university in nation to use landfill gas as primary energy source 2UNH becomes first university in nation to use landfill gas as primary energy source 3Leaderless movement proves illusive 2
NuCycl DAPI Kit Application: Flow Cytometry Reactivity: Human...
Anti-hamster Ig HRP detection kit...
Request Info...
The 50 ml Magnetic Separation Rack is designed to be used for small-scale separations using magnetic particles. ,Capacity: four 50 ml tubes. ,Magnets: Neodymium rare earth permanent magnets....
Biology Products: