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Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression,,,Vector

r inducible bacterial expression, the CMV promoter and enhancer region for constitutive mammalian expression, and tandem consensus sequences for optimal translation initiation in both systems. The c-myc epitope and HIS6 purification tags at the carboxy terminus of the expressed protein allow proteins to be detected and purified, respectively. The tags can be separated from the protein encoded by the cDNA via a thrombin cleavage site.

Acknowledgments

The authors would like to thank the DNA sequencers at Phenogenex for their excellent technical assistance.

REFERENCES

  1. Shine, J., et al. (1974) Proc. Natl. Acad. Sci. USA 71: 1342-1436.

  2. Kozak, M. (1986) Cell 44: 283-292.

  3. Padgett, K., et al. (1996) Gene 161: 31-35.

  4. Evan, G.I., et al. (1985) Mol. Cell Biol. 5: 3610-3616.

  5. Hochuli, E., et al., (1987) J. Chromatog. 411: 177.

  6. Chalfie, M., et al. (1994) Science 263: 802-905.

* Patent pending


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