Navigation Links
Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression,,,Vector

(Figure 5). The GFP was biologically active during protein purification and cleavage (data not shown).

Fig.5

Unique Site for Nucleotide Sequence Insertion

The unique Not I site between the cDNA insert and thrombin cleavage site in the pDual GC vector allows any desired nucleotide sequence to be inserted (Figure 1). Not I was chosen as it is estimated to occur only once in 100,000 bases in the human genome and is, therefore, unlikely to be present in most cDNA. For example, inserting nucleotide sequences that encode GFP permits visualization of cellular proteins in mammalian cells. Alternatively, sequences encoding a translation stop codon would result in translational termination at the inserted sequence.

Fast and Easy Subcloning

Unique Pme I sites flanking the cloned cDNA can be used to subclone the RBS/Kozak and cDNA sequences into other vectors. Pme I is estimated to occur only once in 70,000 bases in the human genome and, hence, is unlikely to be present in most cDNA. Digestion with Pme I creates blunt ends that can either be directly ligated to other blunt ends or to adaptors containing the desired ends. Being able to directly subclone the sequence-verified cloned cDNA enables the known nucleotide sequence to be preserved.

Conclusions

The GeneConnection expression-tested clones offer sequence-validated and mammalian expression-tested human cDNA in a dual prokaryotic and eukaryotic expression vector. Using sequence-validated clones makes it possible to draw valid conclusions based on the function of a protein. The dual expression vector eliminates the need to obtain and validate separate expression vectors. The vector contains a hybrid bacterial promoter fo
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Human Primary Preadipocytes and Differentiated Adipocytes
2. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
5. Human Universal cDNA Library Array I
6. Map and Link Human Genetic Disorders with SSLP Analysis
7. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
8. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
9. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
10. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
11. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
Post Your Comments:
(Date:11/26/2014)... Bio-Techne Corporation (NASDAQ: TECH ) ... to serve in a newly created position of ... Gavin will be responsible for managing the operations of ... business acquired in July 2014 and the recently acquired ... -based ProteinSimple develops and commercializes proprietary systems and ...
(Date:11/26/2014)... , Nov. 25, 2014  Roka Bioscience, Inc. (NASDAQ: ... advanced testing solutions for the detection of foodborne pathogens, today ... the Piper Jaffray  26 th Annual Healthcare Conference on ... held at The New York Palace in New ... material information concerning the Company, its operations, strategies and prospects ...
(Date:11/24/2014)... DUBAI , UAE, November 24, 2014 ... prestigious "Sheikh Hamdan Bin Rashid Al Maktoum Awards for ... in Dubai, United Arab Emirates , ... http://photos.prnewswire.com/prnh/20141124/717861 ) , The Carter Center ... Medical Services is actively involved in numerous programs that ...
(Date:11/24/2014)... Nov. 24, 2014 VolitionRx Limited (OTCQB: ... for cancer and other conditions, today announced that data from ... Science for Business BioWin Day 2014, being held November 26, ... come from VolitionRx,s lung cancer pilot study, the samples for ... Hospitalier Universitaire (CHU) de Liege in Belgium ...
Breaking Biology Technology:Bio?Techne Announces Appointment Of Robert Gavin As Leader Of New Protein Platforms Division 2Bio?Techne Announces Appointment Of Robert Gavin As Leader Of New Protein Platforms Division 3Roka Bioscience to Present at the Piper Jaffray Healthcare Conference 2Five US Winners Among Recipients of Hamdan Medical Awards 2VolitionRx to Present Positive Data from Pilot Lung Cancer Study at BioWin Day 2014 2VolitionRx to Present Positive Data from Pilot Lung Cancer Study at BioWin Day 2014 3VolitionRx to Present Positive Data from Pilot Lung Cancer Study at BioWin Day 2014 4VolitionRx to Present Positive Data from Pilot Lung Cancer Study at BioWin Day 2014 5VolitionRx to Present Positive Data from Pilot Lung Cancer Study at BioWin Day 2014 6
... Madison, Wisconsin was ranked the number-one city in the ... followed by Raleigh-Durham and Austin, in the publications' ... ,In 2003, the city ranked 5th and was selected ... on criteria that included education of the workforce, percent ...
... been a face-to-face interaction in which people meet for ... technology boom, the networking landscape has changed thanks to ... and online social networking. People no longer have to ... , ,The Midwest has been generously affected by this ...
... Wisconsin Technology Network is committed to helping improve patient ... technology. We salute the administration and the efforts of ... adoption health technology. If you are interested in ... following event. , ,The University of Wisconsin ...
Cached Biology Technology:Forbes highlights Wisconsin biotech, names Madison #1 business city 2Using Technology to Professionally Network in the Midwest 2Using Technology to Professionally Network in the Midwest 3Using Technology to Professionally Network in the Midwest 4The doctor is in: Thompson prescribes better health information technology 2The doctor is in: Thompson prescribes better health information technology 3The doctor is in: Thompson prescribes better health information technology 4
(Date:11/21/2014)... NEW YORK , Nov. 19, 2014  Earlier ... Board at New York College, and one of the ... for biosensor signals that are transmitted from Smartphones to ... there were Smartphones and has one of the earliest ... are discussing its usage in the military, child care, ...
(Date:11/18/2014)... Nov. 18, 2014  The Secure Identity & ... today jointly announce the formation of The Airport ... its Identity and Biometric Entry and Exit ... received official support from BORDERPOL, the international non-profit ... and provide expertise regarding border security, traveler and ...
(Date:11/15/2014)... 13, 2014  While we may still be a few ... in "Star Trek" to gain instant access to all that ... with smartphones and tablets for monitoring and measuring our health ... This may seem a tad Orwellian to some, but a ... of these technological opportunities into their healthcare regime. ...
Breaking Biology News(10 mins):Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3Americans May Be Ready for a Brave New World of Healthcare 2Americans May Be Ready for a Brave New World of Healthcare 3Americans May Be Ready for a Brave New World of Healthcare 4
... Ursolic acid (chemical name 3-hydroxy-12- ursen-28-oic acid) is ... has anti-oxidative, anti-inflammatory, anti-apoptotic, and anti-scarring effects, and ... repair of injured neurons.However, no reports have explored ... study by Prof. Jiajun Chen and team from ...
... Payout, Inc. (OTC: GOHE) has signed an agreement with ... based multi-modal biometric identity management solutions, whereby ImageWare will ... platform, which will enable a fully-validated, feature-rich, security solution ... IaaS Trusted Public S5 platform. Global Payout and ImageWare ...
... funding by the National Institutes of Health to develop ... to changing environments to improve human capabilities and enhance ... million over the next five years, subject to the ... year of NIH,s participation in the National Robotics Initiative ...
Cached Biology News:ImageWare Systems Provides Next Generation Cloud Identity Management and Authentication Services to Global Payout's MoneyTracTM Consolidated Payment Gateway 2ImageWare Systems Provides Next Generation Cloud Identity Management and Authentication Services to Global Payout's MoneyTracTM Consolidated Payment Gateway 3ImageWare Systems Provides Next Generation Cloud Identity Management and Authentication Services to Global Payout's MoneyTracTM Consolidated Payment Gateway 4NIH funds development of novel robots to assist people with disabilities, aid doctors 2NIH funds development of novel robots to assist people with disabilities, aid doctors 3NIH funds development of novel robots to assist people with disabilities, aid doctors 4
Recombinant Equine IL-4, CF...
DALT GRADIENT MAKER,230V, 1 EA. Category: 2D DALT Systems....
delta-like 1 homolog (Drosophila)...
... mutS are mismatch repair minus strains of ... repair of the newly synthesized unmethylated strand ... making them helpful in such systems as ... Systems. Both ES1301 and BMH 71-18 mutS ...
Biology Products: