Navigation Links
Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression,,,Vector


Study protein function more efficiently with standardized clones

Rebecca L. Mullinax Heidi Davis David T. Wong Kelly Wynne Vilma Nioko
Leonardo DeLeon SanDEe Soares Joseph A. Sorge Edward Marsh
Stratagene

Spencer Stevens Chris Hansen Brian Schilling
Phenogenex

Stratagene now offers sequence-validated and expression-tested human cDNA in a single dual- expression vector. These clones eliminate the time spent cloning, sequencing, and expression testing new genes and allow gene analysis experiments to begin immediately. The versatile dual-expression vector permits proteins to be expressed and detected in mammalian cells and proteins to be expressed in and purified from bacterial cells.

In the near future, the human genome will be completely sequenced. The next and more challenging step will be to characterize the biological role of each gene and the way in which the encoded protein functions in the cell. To facilitate this characterization, Stratagene has cloned the open reading frame (ORF) of selected human cDNA into a dual-expression vector. Potential uses for these expressed proteins include analyzing protein function, defining both protein-protein and protein-DNA interactions, elucidating pathways, studying protein degradation, determining the effects of over-expression, and preparing antigen.

Fig.1

The pDual GC expression vector* was designed for high-level protein expression in mammalian and bacterial cells ( Figure 1). The dominant selectable marker is the neomycin phosphotransferase gene, which is under the dual control of the b-lactamase and SV40 promoters in bacterial and mammalian cells, respectively. The tandemly arranged bacterial Shine-Dalgarno1 and mammalian Kozak
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Human Primary Preadipocytes and Differentiated Adipocytes
2. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
5. Human Universal cDNA Library Array I
6. Map and Link Human Genetic Disorders with SSLP Analysis
7. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
8. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
9. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
10. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
11. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
Post Your Comments:
(Date:1/14/2014)... , Jan. 14, 2014  3D Communications, a leading provider of ... regulatory, business, and media events in the United States ... former associate Virginia Cox , JD, is returning to the ... Virginia Cox re-joins 3D after more than two years of ...
(Date:1/14/2014)... BETHESDA, Md. , Jan. 14, 2014  Organovo announced ... from the National Institutes of Health (NIH) to help ... effective treatments to patients on a faster timeline. Organovo ... Sciences (NCATS) and the National Eye Institute (NEI) to ...
(Date:1/14/2014)... Global Record Systems, LLC, (GRS), a ... for patients, physicians, the biopharmaceutical industry, regulators, payers, ... signing of a three-year Research Collaboration Agreement (RCA) ... This initiative is designed to generate disruptive ...
(Date:1/14/2014)... (PRWEB) January 14, 2014 During the 1600’s ... known as “The Doctor’s Plague.” In this time period, doctors ... This led, at times, to the death of vulnerable patients. ... don’t know that they may be unwittingly transmitting herpes viruses ...
Breaking Biology Technology:Former FDA Associate Commissioner Returns To 3D Communications 2Organovo Announces Collaboration with National Institutes of Health 2Organovo Announces Collaboration with National Institutes of Health 3Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 3
... Networks Corporation s recent acquisition of Network Engineering ... brings NEAs strength in the key technology of voice ... announced it bought the Wausau-based IT firm last week ... keep all of NEAs 22 employees. , ,"The combination ...
... interested in achieving greater efficiencies in product development and ... the University of Wisconsin-Madisons Center for Quick Response ... in Biotech briefing, co-hosted by the center and ... held Wednesday, Aug. 18 from 9 a.m. to noon ...
... aimed at protecting businesses , ,MADISON, Wis. ... with MasterCard to help battle online identity ... , ,Under the arrangement, NameProtect will use its technology ... other forms of payment fraud. Phishing describes the act ...
Cached Biology Technology:Berbee acquisition could expand VoIP in Wisconsin 2NameProtect and MasterCard unite to combat online fraud, phishing 2
(Date:4/17/2014)... warning on the possible effects of gases such as ... that of carbon dioxide. Now a team of Swiss-German ... regenerable electron acceptors which helps explain why large amount ... released to the atmosphere. However, there are worries that ... a vicious cycle to release large amounts of methane ...
(Date:4/17/2014)... the genetic information of DNA is used to ... numerous different functions in living organisms. Messenger RNA ... expression, by relating the genetic information of DNA ... , By examining the different types and ... at a given time, researchers can determine which ...
(Date:4/17/2014)... uncovered a new way the immune system may fight ... to use immune cells to treat illness. , The ... immunological equivalent of "neighborhood police" specialized squads of ... instead of an entire city, the body. , Scientists ... have shown that the liver, skin and uterus each ...
Breaking Biology News(10 mins):Methane climate change risk suggested by proof of redox cycling of humic substances 2Rapid and accurate mRNA detection in plant tissues 2Some immune cells defend only 1 organ 2
... 19, 2013) Columbia University College of Physicians ... announced that the Mortimer D. Sackler, M.D. Prize ... awarded to Carla Shatz, PhD, the Sapp Family ... Palo Alto, California. Dr. Shatz,s work has advanced ...
... Researchers at Plymouth University, UK, believe that findings from ... between the Early Jurassic era of 180 million years ... Through geology and palaeontology, they,ve shown how higher ... marine communities, and that while the Jurassic seas eventually ...
... elementary school students are expected to attend an INSECT EXPO ... PM on Monday, February 25 at the start of the ... Society of America (ESA), which will be held February 25-28 ... This free educational event will reinforce students, science ...
Cached Biology News:Pioneer in neural development honored with the Mortimer D. Sackler, M.D. Prize 2Pioneer in neural development honored with the Mortimer D. Sackler, M.D. Prize 3Pioneer in neural development honored with the Mortimer D. Sackler, M.D. Prize 4Jurassic records warn of risk to marine life from global warming 2
... 220 utilizes utilizies CTI Molecular Imaging's ... superior resolution, sensitivty, and a large ... the flexibility to image multiple mice ... microPET is a dedicated small animal ...
... I provides a convenient and affordable alternative ... Used with an eight-port disposable manifold, the ... with multi-channel pipettes. The eight-port manifold, designed ... of 100, 200, 250, or 300mL with ...
... Buffered) has been developed specifically for the ... HRP Stabilizer prevents the loss of ... the antibody or protein antigen portion of ... 0.2 microns., ,Contents: Aqueous, protein-containing diluent ...
384 Well PCR Plate Carrier for Thermo-Sealer...
Biology Products: