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Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System

n source without any hardware modifications or post-flow column addition. To eliminate potential explosion hazards associated with normal phase APCI LC/MS/MS, nitrogen gas was utilized for the auxiliary and nebulizer gas. This experiment resulted in a sensitive chiral analysis of verapamil and methylphenidate with an assay sensitivity for each enantiomer of 0.05 ng/mL. Fluoxetine did not exhibit similar sensitivity due to poor column performance requiring long retention times and high DEA levels to achieve a reproducible enantiomer separation.


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