Navigation Links
Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

e, but distinct proteins at lower molecular weight did not resolve as well or were less concentrated in the ATCC strain than the CSF strain. Two major proteins (p15 and p53) are represented in the virulent (CSF) strain but do not appear in the ATCC strain, while at least one protein (p37) appears to be differentially expressed in the ATCC strain.


Conclusions
The techniques outlined here demonstrate the effectiveness of sequential extraction in obtaining bacterial proteins from different compartments of the cell. Combined with 2-D PAGE techniques, the ReadyPrep sequential extraction kit allowed cell-associated and cytoplasmic proteins to be effectively separated. In addition to the development of techniques to separate different bacterial protein components, we have successfully separated proteins from both virulent and nonvirulent strains of F. psychrophilum by 2-D PAGE. The use of the PROTEAN IEF cell allowed rapid throughput of samples and exceptional control over focusing conditions. The separation of distinct proteins using these methods has allowed the comparison of potential virulence factors between two bacterial isolates grown under identical conditions. Further characterization is needed and will focus on comparison of differentially expressed proteins to active proteases (potential virulence components) and immunodominant bacterial antigens. Protein sequencing and eventual gene isolation may lead to the development of an efficacious recombinant vaccine for aquaculture, or may allow screening of the many F. psychrophilum isolates for potential vaccine candidates. This approach has the potential to substantially advance our current understanding of
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Separation Reproducibility With the BioLogic Chromatography Systems
2. Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A
3. Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
4. Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System
5. BD IMag Human Regulatory T Lymphocyte Separation Set for Isolation of Treg Cells
6. Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80
7. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
8. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
9. Performance Comparisons of Commercial RT-PCR Systems
10. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
11. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
Post Your Comments:
(Date:4/30/2015)... Calif. , April 30, 2015  Asterias Biotherapeutics, ... the emerging field of regenerative medicine, today announced that ... on Thursday, May 7, 2015 after the close of ... conference call and webcast on Thursday, May 7, 2015 ... discuss the results and recent corporate developments. ...
(Date:4/30/2015)... Available in select spas in the ... Phytomer’s DOUCEUR INTEMPORELLE Restorative Shield Cream provides immediate ... the weakening threat of inflammaging, aging directly caused ... star ingredient GLYCOSEA, a complex of restructuring plankton ... soothing minerals and trace elements. GLYCOSEA works to ...
(Date:4/29/2015)... According to a new market research report “Vibration ... Component (Accelerometer, Proximity Probe, & Others), Application (Aerospace ... Others), & Geography - Global Forecast to 2020", ... Million by 2020, at a CAGR of 6.6%. ... figures spread through 205 pages and in-depth TOC ...
(Date:4/29/2015)... 29, 2015 Cambrex Corporation (NYSE: CBM ) ... on Friday, May 1, 2015 before the market opens. ... will host a conference call to discuss the financial results. ... , Friday, May 1, 2015 at 8:30 a.m. ... for U.S.1-719-325-2454 for International  Passcode:  7993632 Dial-in Replay: ...
Breaking Biology Technology:Asterias Biotherapeutics to Report First Quarter Results on May 7, 2015 2Asterias Biotherapeutics to Report First Quarter Results on May 7, 2015 3Phytomer introduces DOUCEUR INTEMPORELLE Restorative Shield Cream 2Phytomer introduces DOUCEUR INTEMPORELLE Restorative Shield Cream 3Vibration Monitoring Equipment (Portable & Non-Portable) Market worth $1,453.76 Million by 2020 - New Report by MarketsandMarkets 2Vibration Monitoring Equipment (Portable & Non-Portable) Market worth $1,453.76 Million by 2020 - New Report by MarketsandMarkets 3Vibration Monitoring Equipment (Portable & Non-Portable) Market worth $1,453.76 Million by 2020 - New Report by MarketsandMarkets 4
... life,sDHA™ and the National Center for ... year for Beautiful Minds: Finding Your Lifelong Potential ... Americans to develop and maintain healthy, beautiful minds by incorporating ... health – diet and nutrition, physical health, mental health and ...
... ,   - CNIT Paris La ... of services offered by the ALTEN Group to the Telecom industry ... in April 2011. The integration is a success, and the telecom ... "Broadband World Forum 2011" exhibition at CNIT Paris La Defense. ...
... This release is available in German . ... plastic foils, have excellent potential to lower the manufacturing ... cost, thus enabling affordable solar electricity in the near ... films have been lacking behind in performance compared to ...
Cached Biology Technology:Proactive Brain Health Critical as Americans' Life Expectancy Reaches All Time High 2Proactive Brain Health Critical as Americans' Life Expectancy Reaches All Time High 3Proactive Brain Health Critical as Americans' Life Expectancy Reaches All Time High 4ALTEN and Calsoft Labs at the BWF 2011 Exhibition 2How to produce flexible CIGS solar cells with record efficiency 2
(Date:4/6/2015)... 6, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... company focused on the growing mobile commerce market, ... 62/143028 for MINIATURE, MULTI-PURPOSE ANTENNA METHOD AND ... NXT-ID introduces a new revolutionary method and ... the use of miniature antenna modules for multiple ...
(Date:4/1/2015)... 1, 2015  NXT-ID, Inc. (NASDAQ: NXTD ) ... on the growing mobile commerce market, announces the second ... is underway to early access pre-order customers. ... at retail outlets including Walmart, Target, AT&T, Dunkin, Donuts, ... was accepted at all outlets and very easy to ...
(Date:3/26/2015)... The Granite Club, ... club, today announced it has implemented a state-of-the-art access ... for members and staff, while restricting access to authorized ... selected FST,s IMID Access system because it has the ... and staff, in addition to unparalleled security," said ...
Breaking Biology News(10 mins):NXT-ID Patents Miniature, Low Power Wireless Magnetic Stripe 2NXT-ID Patents Miniature, Low Power Wireless Magnetic Stripe 3NXT-ID Patents Miniature, Low Power Wireless Magnetic Stripe 4NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 2NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 3NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 4Granite Club First Private Club in North America to Implement FST Biometrics Secure Access Solution, as Part of Multi-million Dollar Expansion 2
... CAMBRIDGE, Mass. -- About one-third of the human population is ... most of them don,t know it. Though Toxoplasma ... harmful to individuals with suppressed immune systems, and to fetuses ... are found in dirt and easily infect farm animals such ...
... cancer is the sixth most common type of cancer and ... women without any known risk factors. Now, researchers at Fox ... of precancerous cells in the mouth and thus promote the ... The new results, published in the January issue of ...
... Anatomist,s (AAA) will present its 2011 Young Investigator Awards ... in their respective fields and show remarkable promise of ... lecture at the AAA Annual Meeting during Experimental Biology ... Washington Convention Center, Washington, DC, Room 102A). ...
Cached Biology News:MIT researchers study the danger of toxoplasma parasites 2Estrogen may help precancerous cells spread in oral cavity 2American Association of Anatomists announces young investigator award winners 2
A compact, high-speed single tube reader unit designed for benchtop 2D barcode reading. The unit can be used with all of ABgene's 2D barcoded tubes, including the 2ml CryoCode tube....
PTFE/red rubber septum silver aluminum seal ,11 mm seal diam. ...
... stops PCR carry-over contamination, which prevents ... PCR products from previous PCR amplifications ... which improves amplification ,Optimized for use ... GeneAmp instrument systems , The GeneAmp ...
... hydrolytic enzyme that catalyzes the conversion,of ... maltose, maltotriose and,dextrins. The level of ... human,body are of clinical importance,1-3 while ... for industry.4, The EnzChek Amylase Assay ...
Biology Products: