Navigation Links
Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

Kenneth D. Cain1 , Leslie Grabowski1 , and Scott E. LaPatra2
1 Department of Fish and Wildlife Resources, University of Idaho, Moscow, ID USA
2 Clear Springs Foods Inc., Research Division, PO Box 712, Buhl, ID USA


Introduction
Coldwater disease (CWD) is a worldwide problem in commercial (foodfish) aquaculture as well as public resource enhancement hatcheries, and is caused by the gramnegative bacterium Flavobacterium psychrophilum. Juvenile trout and salmon infected with this disease experience high mortalities, and surviving fish often exhibit skeletal deformities associated with spinal compression. Aquaculture vaccines have become important tools in preventing fish diseases, but a commercial vaccine is not currently available for CWD. Development in this area has likely been hampered by altered expression of virulence factors among different strains of F. psychrophilum and an incomplete understanding of the immune response of fish to this pathogen. It has been noted that protease differences exist between strains (Dalsgaard 1993, Bertolini et al. 1994, Madsen and Dalsgaard 1999), but the identities of these components and roles they play in the fish immune response are unknown. Significant protection has been observed under laboratory conditions following immunization with F. psychrophilum (Holt 1988, Obach and Laurencin 1991), but antigenicity appears to be strain dependent.

A study is currently underway in our laboratory using twodimensional polyacrylamide gel electrophoresis (2-D PAGE) to identify antigens and virulence factors of this bacterium that may be targeted for the development of an efficacious vaccine for the aquaculture industry. 2-D gel profiles of virulent and nonvirulent strains are being compared to iden
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Separation Reproducibility With the BioLogic Chromatography Systems
2. Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A
3. Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
4. Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System
5. BD IMag Human Regulatory T Lymphocyte Separation Set for Isolation of Treg Cells
6. Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80
7. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
8. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
9. Performance Comparisons of Commercial RT-PCR Systems
10. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
11. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
Post Your Comments:
(Date:8/28/2014)... , Aug. 28, 2014 /PRNewswire/ ... new market research report is ... Whole Exome Sequencing Market ... Preparation, Target Enrichment), by Services ... Synthesis), by Application (Cancer, Monogenic ...
(Date:8/28/2014)... MENLO PARK, Calif. , Aug. 28, 2014 ... company focused on developing novel mid- to late-stage ... raised $7.0 million in its first round of ... AshHill Biomedical Investments and Hercules Bioventures with participation ... biotech entrepreneur and pharmaceutical executive, Dr. Larry ...
(Date:8/28/2014)... Boston, MA (PRWEB) August 28, 2014 ... of wholesale construction materials, selected SoundConnect ... of their cloud based audio and web conferencing ... configured to support PrimeSource’s employees’ needs. SoundConnect ... technologies. , PrimeSource Building Products will be ...
(Date:8/27/2014)... Aug. 27, 2014 Rhythm, a biopharmaceutical company ... deficiencies that result in metabolic disorders, announced today that ... with the U.S. Securities and Exchange Commission (SEC) relating ... its common stock. The number of shares to be ... not yet been determined. Citigroup and Cowen ...
Breaking Biology Technology:Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 2Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 3Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 4Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 5Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 6Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 7Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 8Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 9Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 10Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 11Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 12Whole Exome Sequencing Market by Product (Systems, Kits (Library Preparation, Target Enrichment), by Services (Sequencing), by Technology (Sequencing by Synthesis), by Application (Cancer, Monogenic disorders) - Global Forecast to 2018 13Armetheon Completes $7.0 Million Series A Financing 2Armetheon Completes $7.0 Million Series A Financing 3PrimeSource Chooses SoundConnect as Collaboration Provider 2Rhythm Files Registration Statement for Proposed Initial Public Offering 2
... DALLAS, January 9, 2012 According to ... Trends and Global Forecasts (2011-2016)" , published by MarketsandMarkets ... fastest growing segment of crop protection market. This growth ... treatment as crop protection practice. This growth is particularly ...
... SFJ Pharma Ltd. II (President & CEO: Robert F. ... a collaborative development agreement with Pfizer Inc. to conduct ... investigational agent axitinib for the adjuvant treatment of patients ... following nephrectomy. Axitinib is an oral and ...
... 2012 Sutro Biopharma, a biopharmaceutical company developing novel ... announced that it has appointed James A. Wells, Ph.D., ... scientific advisory board (SAB).  Prof. Wells will serve as ... together two leading scientific experts in the field of ...
Cached Biology Technology:MarketsandMarkets: Global Seed Treatment Market worth $3430.3 Million by 2016 2MarketsandMarkets: Global Seed Treatment Market worth $3430.3 Million by 2016 3SFJ Pharma Ltd. II, Announces Agreement With Pfizer to Develop Axitinib for Adjuvant Treatment of Renal Cell Carcinoma 2Sutro Announces Newly Formed Scientific Advisory Board 2Sutro Announces Newly Formed Scientific Advisory Board 3
(Date:8/29/2014)... in German . ... is considered the oldest form of communication. Acting as ... example, the sexual attraction between males and females. Fish ... coordinate reproductive behavior in males and females. Scientists at ... Algarve in Faro, Portugal, and at the Max Planck ...
(Date:8/29/2014)... twist them, fold them: modern materials that are light, ... as artificial skin or electronic paper. , Making such ... but a new way of working with copper nanowires ... , Previous success in the field of ultra-lightweight ... precious gold and silver nanowires. , By turning ...
(Date:8/28/2014)... Entomological Society of America (ESA) is pleased to announce ... Member of the Society. Honorary Membership acknowledges those who ... significant involvement in the affairs of the Society that ... are selected by the ESA Governing Board and then ... be honored at the Awards Ceremony at Entomology 2014, ...
Breaking Biology News(10 mins):Ready for mating at the right time 2Ready for mating at the right time 3Copper shines as flexible conductor 2Fred Baxendale selected as Honorary Member of the Entomological Society of America 2
... the University of Leeds, has found that a common ... monoxide poisoning. Animal studies have shown that the ... deaths from arrhythmias irregular or abnormally paced heartbeats ... The findings could have important implications for the development ...
... August 6, 2012 Individuals with mutations in BRCA1 and ... and ovarian cancers. Families at risk have been seeking ... but the standard method of direct sequencing is labor-intensive, costly, ... BRCA2 genes. A group of Canadian scientists has developed ...
... is able to source food from the land as well as ... University of London has found. Scientists analysed the behaviour of ... the water level of the lake was low, the crayfish found ... journal PLoS ONE today (3 August 2012). Lead ...
Cached Biology News:Anti-angina drug shows protective effects from carbon monoxide 2Anti-angina drug shows protective effects from carbon monoxide 3New method provides fast, accurate, low cost analysis of BRCA gene mutations in breast cancer 2Crayfish species proves to be the ultimate survivor 2