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Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

umn) and the internal standard alprazolam-d5 at 10 pg/μL (50 pg on-column). Figure 1 shows extracted ion chromatograms of the most intense product ions observed in the full-scan MS/MS spectra for temazepam (m/z 255), alprazolam (m/z 274 + 281), and the internal standard, alprazolam-d5 (m/z 279 + 286). Figure 4 shows the full-scan MS/MS spectra of temazepam, and alprazolam at a concentration level of 1 pg on-column and the internal standard, alprazolam-d5 (50 pg on-column). This concentration level exhibited a signal-to-noise ratio of 7 to 1 for temazepam and 9 to 1 for alprazolam.

The calibration curve for alprazolam over the entire range of the assay is shown in Figure 5, with the linearity over the dynamic range from 500 fg to 5 ng on-column. The correlation coefficient is 0.9991. The precision values for the alprazolam assay are displayed in Table 1. The % CV or RSDs show that the LCQ Deca XP Plus ion trap mass spectrometer is capable of quantitating analytes at low picogram amounts in plasma. Table 2 shows that the limit of detection (LOD) and quantitation (LOQ) for the antipsychotic drug alprazolam are determined to be 500 fg and 1 pg, respectively, whereas that for its analog temazepam are 1 pg and 2.5 pg, respectively.

Conclusions
These measurements demonstrate that the LCQ Deca XP Plus ion trap mass spectrometer can be used to effectively quantify compounds in biological matrices. Benzodiazapines were successfully quantified with linearity over four orders of magnitude for alprazolam. The CV was around 5-7% except near the LOQ, where the CV was 15%. The LOD and LOQ for alprazolam is 500 fg and 1 pg, respectively whereas that for temazepam are 1 pg and 2.5 pg, respectively.


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