Navigation Links
Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

ctron).
Injection volume: 5 μL
Flow Rate: 400 μL/min
Mobile phase A: water containing 10 mM ammonium acetate B: acetonitrile containing 0.1 % formic acid
Gradient: 45% B for 3 minutes

Mass Spectrometer
Ionization mode: Positive electrospray ionization (ESI)
Capillary temperature: 350C
Spray voltage: 4.5 kV
Sheath gas: 45 units Sweep gas: 10 units
Maximum inject time: 400 ms
Isolation width: 1.0
Collision energy: 40%
Number of segments: 2
Segment 1: Full-scan MS/MS m/z 301.2 → m/z [100-305]
Segment 2: Full-scan MS/MS Scan event 1 m/z 309.2 → m/z [100-311] Scan event 2 m/z 314.2 → m/z [100-320]

Analysis
The standard samples were analyzed with five replicate injections at each concentration level and a blank (MeOH) injection between each level.

Results and Discussion
Eleven calibration standards over a concentration range of four orders were used to evaluate the quantitative performance of the LCQ Deca XP Plus mass spectrometer. Figure 1 shows the extracted ion chromatograms from full-scan MS/MS of alprazolam (500 fg on-column) and alprazolam-d5 internal standard (50 pg on-column) in protein precipitated bovine plasma. These chromatograms were generated by summing the two most intense product ions observed in the full-scan MS/MS spectra (Figure 2) acquired for the analyte (m/z 274 + 281) and the internal standard (m/z 279 + 286). The LOD was determined to be 500 fg for alprazolam, with a signal-tonoise of 5 to 1.

Figure 3 shows the extracted ion chromatograms from full-scan MS/MS of temazepam and alprazolam spiked in protein-precipitated bovine plasma at 0.2 pg/μL (1 pg oncol
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Improved Sensitivity for Staining RNA with SYBR Gold Stain
2. Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology
3. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
4. Increased Sensitivity in Microarray Analysis
5. Femtogram Sensitivity of the Antipsychotic Drugs Raclopride and Ritanserin Using a Varian 1200L LC/MS/MS
6. Maintaining High Sensitivity when Working with Small-Volume Samples
7. High Sensitivity, Wide Dynamic Range, Horseradish Peroxidase (HRP) ELISA Using the LMax Microplate Luminometer (MaxLine Application Note #41)
8. Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery
9. Trace Analysis Optimizing Sensitivity Finnigan Surveyor PDA Detector
10. A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity
11. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
Post Your Comments:
(Date:7/10/2014)... -- According to the International Atomic Energy Agency ... from terrorists acquiring sufficient quantities of plutonium or ... nuclear explosive device. The IAEA also notes that ... gram-level quantities, which can be challenging to detect ... new study appearing this week in the ...
(Date:7/10/2014)... 17, 2014 Using microscopic polymer light resonators ... researchers at MIT,s Quantum Photonics Laboratory have developed ... the parts-per-billion range. Optical sensors are ideal for ... signal-to-noise ratio, compact, lightweight nature, and immunity to ... had been developed before, the MIT team conceived ...
(Date:7/10/2014)... been extremely challenging because there are many genetic variants ... disease. Now investigators reporting in the July 3rd issue ... describe a strategy that may help reveal how ... or environmental exposures to affect the development of the ... may predispose individuals to schizophrenia. , The work ...
(Date:7/10/2014)... lithium-ion batteries that power our laptops and electric vehicles ... single charge with the help of a sponge-like silicon ... replace the graphite traditionally used in one of the ... the energy storage capacity of graphite. A paper describing ... published today in Nature Communications . , "Silicon ...
Breaking Biology Technology:Sensitive detection method may help impede illicit nuclear trafficking 2Swell new sensors 2New strategy could uncover genes at the root of psychiatric illnesses 2Silicon sponge improves lithium-ion battery performance 2Silicon sponge improves lithium-ion battery performance 3
... adison, Wis. - Third Wave Technologies, Inc. ... a share, for the second quarter of 2006, but robust ... upward in a key market. , ,Third Wave, which develops ... that revenue rose by 15 percent for the quarter ended ...
... Madison, Wis. - A United States Air Force contract ... not only provide a Madison aerospace research and product development ... it to propel something much larger. , ,The U.S. ... Orbital Technologies Corp. (Orbitec) for the development of ...
... - Thanks to a classified contract from the ... company headquartered here, reported record revenues and earnings for ... the closing of its manufacturing facility in England. , ... of lower-than-expected earnings in the fourth quarter, which caused ...
Cached Biology Technology:Third Wave reports another loss, stronger sales 2Third Wave reports another loss, stronger sales 3Orbitec will use $25 million Air Force contract to make small launch vehicle 2Defense contract helps Plexus gain record profits 2
(Date:7/11/2014)... the Department of Energy,s Oak Ridge National Laboratory ... R&D Magazine in recognition of the year,s top ... tremendous value of our National Labs," said Secretary ... the National Labs continues to help our nation ... and technological innovations necessary to remain globally competitive." ...
(Date:7/11/2014)... The University of Chicago is creating a new ... at the University,s Institute for Molecular Engineering and ... million donation from the Millicent and Eugene Bell ... will reside within the Institute for Molecular Engineering. ... research project at the MBL,s Eugene Bell Center ...
(Date:7/11/2014)... The Chinese University of Hong Kong, BGI and other ... to salt tolerance, with implication for improving this important ... online in Nature Communications provides an effective ... , Soybean is an important crop for the world. ... less genetic diversities than their wild counterparts. Among the ...
Breaking Biology News(10 mins):ORNL wins eight R&D 100s 2ORNL wins eight R&D 100s 3ORNL wins eight R&D 100s 4New professorship in tissue engineering links molecular engineering, marine biology 2BGI reports a novel gene for salt tolerance found in wild soybean 2
... the University of Leeds are developing methods of helping ... chemicals such as vinegar. , The harmful chromium ... from former textiles factories, smelters, and tanneries have been ... problems with the kidneys, liver, lungs and skin. , ...
... but researchers in China have discovered that chicken manure can ... in the International Journal of Environment and Pollution ... 50% more crude oil than soil lacking the guano. ... University, China, point out that contamination of soil by crude ...
... comes to solving complex problems, Geoffrey von Maltzahn, MIT ... solutions. Finding inspiration in systems that evolution has produced, ... society,s biggest challenges: improving tumor detection and therapeutic delivery ... patients. Today, the 28-year-old Ph.D. candidate in ...
Cached Biology News:Cleansing toxic waste -- with vinegar 2Fowl soil additive breaks down crude oil 2MIT student develops new innovations to selectively kill cancer cells 2MIT student develops new innovations to selectively kill cancer cells 3MIT student develops new innovations to selectively kill cancer cells 4
Mouse monoclonal [3G131] to Desmoglein 1 ( Abpromise for all tested applications). entrezGeneID: 1828 SwissProtID: Q02413...
Mouse monoclonal [2D7/1] to E.coli LPS ( Abpromise for all tested applications). Antigen: Tissue / cell preparation: Escherichia coli J5 cells....
COMING SOON: Anti-Mouse C5/C5b, Clone BB5.1. Monoclonal Antibody...
KAI 1 (G-2)...
Biology Products: