( n the tumor cellsand the host and t...)Sensitive detection of tumor cells in,,,peripheral blood of carcinoma patients,,,by a reverse transcription PCR method

n the tumor cells and the host, and this interaction adds to the extreme complexity of the events associated with tumor dissemination. Spreading of tumor cells into the blood circulation from either primary tumors or subsequently from the lymphatic origin may be a result of dissemination of the primary tumor or a secondary event during tumor progression. Many of these cells reaching the circulation may be killed by an individual response of the immune system but the metastatic potential of remaining tumor cells cannot be ruled out. The presence of disseminated tumor cells in systemic circulation is an indication for tumor relapse. Their early detection followed by an immediate and possibly even individual therapy may prevent formation of metastases1. Specificity and maximal sensitivity are the main objectives for any method in this field. A very high sensitivity is possible by means of expression markers that are detected and amplified specifically on transcription level. However, due to background from illegitimate transcription in nucleated blood cells, most techniques described encounter the problem of specificity. This can be overcome by specific enrichment of non-blood cells2-6. This Application Note describes a sensitive and specific method to detect testicular, breast and colorectal carcinoma cells from blood. The tumor cell selection and analyses are developed and provided by AdnaGen, using an Agilent 2100 bioanalyzer for detection.
Material and methods
AdnaGen developed a diagnostic system which is based on a twostep procedure:
AdnaTest CancerSelect
A combined method based on immunomagnetic enrichment of tumor cells directly from whole EDTA-blood with an antibody mixture, which was especially designed to isolate breast, testicular and colorectal carcinoma cells.
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