( ...)Sensitive detection of tumor cells in,,,peripheral blood of carcinoma patients,,,by a reverse transcription PCR method

Occurrence of tumor cells in the peripheral blood of individuals suffering from cancer may serve as an early indication that the primary tumor has dispersed from its tissue of origin. Despite defence mechanisms of the organism, individual cancer cells may attach in distant regions and form colonies as an initial step of metastasis formation. Hence, early detection of metastatic potential can be estimated by detecting tumor cells in the blood circulation. Sensitivity and specificity are the main objectives of any method applied for this purpose. Most highly sensitive techniques, encounter an increasing problem of specificity due to background signaling from illegitimate transcription. AdnaGen established a method of tumor cell selection, their specific identification and analysis with a lower limit of detection of at least 2 tumor cells in 5 mL blood (1010 cells). To achieve this we combined the enrichment of tumor cells using a specifically designed antibody mixture with RT-multiplex PCR techniques for the detection of mRNAs encoding for tumor associated markers. Preliminary results of case studies showed the occurrence of tumor cells in blood of carcinoma patients indicating a potential tumor relapse, in some cases several months prior to an elevation of serum tumor markers. AdnaGen provides a sensitive and specific method to detect disseminated tumor cells in peripheral blood of testicular, breast and colorectal carcinoma patients. This innovative method is an option for clinicians as a predictive tool with respect to metastasis formation and may result in an appropriate selection of patients for adjuvant therapy.
Metastases are the major cause of death in cancer patients. Metastases occur as a result of the interaction betwee
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