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Semi Preparative Purification of Proteins

albumin, IgG, IgA, transferrin, haptoglobin, and antitrypsin and dye terminators from human serum sample matrices. This may be performed by the use of Multiple Affinity Removal Systems or by the use of Spin columns. These systems and columns are used to pre-treat samples, before they are injected onto an HPLC column.

Samples should not be suspended in detergent or non volatile buffer containing solutions. Some detergents may bind to reverse phase columns and modify them irreversibly. Samples should not be dissolved in organic solvents, as this may inhibit the proteins from adsorbing to the stationary phase.


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