Navigation Links
Selection of Standards for Bio-Plex Cytokine Assays, Rev A

e range of standards (Figure 4).

The high-sensitivity standards yield a much lower fluorescence intensity signal since the concentration range is much lower. When using the low range standards, reading the assay with the high PMT voltage may improve the sensitivity. Table 5 shows a comparison of the recovery of a low range GM-CSF assay run using the low and high PMT voltage settings. The low PMT setting yielded acceptable recovery down to only 3.13 pg/ml. When the same assay was run at the high PMT setting, accurate recovery down to 0.2 pg/ml was achieved.


Guidelines for Running Cytokine Assays
The overall assay performance must be taken into consideration when determining how to set up both the cytokine assay standards and the Bio-Plex array reader. In Table 6, the guidelines for optimizing the Bio-Plex system (including assays and the array reader) are shown. The standards as well as the PMT voltage chosen for reading the assay may significantly affect results.


Selecting a Custom Set of Standards
The two sets of standards and RP1 target values discussed in this tech note have been validated using the Bio-Plex system and have been shown to yield optimal results. Efficient assay performance may also be achieved using a custom set of standards as long as a few simple rules are followed.

1. Select a minimum of 8 standard curve points.
The logistic regression model requires a minimum of 5 standards for the 4PL and 6 standards for the 5PL to fulfill the analysis requirements (Motulsky 1996). A total of 8 points are recommended to ensure an accurate fit, with a maximum of 12 points (Baud 1993)
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Plasmid Miniprep Kit Selection
3. Universal Guide to DNA Standards
4. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
5. Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
6. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
7. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
8. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
9. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
10. Simultaneous Detection of 8 Cytokines in Mouse or Human Sera
11. The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Selection Standards for Bio Plex Cytokine Assays Rev

(Date:12/22/2014)... Fertility Associates of Memphis has announced a new ... network of more than 20 contributing fertility practices ... access to a national pool of frozen donor ... of Memphis is the only fertility clinic in the ... Dr. William Kutteh , director of Fertility Associates of ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), one of the ... Maroulis, Pharm.D. to the newly created position of Vice ... position, Dr. Maroulis will continue to manage the entire ... multi-site pharmacies as the company expands. Dr. Maroulis has ... he has served in many different capacities in the ...
(Date:12/19/2014)... , Dec. 19, 2014 Bina Technologies, Inc. (Bina, ... today that they have been acquired by Roche (SIX: ... a privately held company that provides a big data ... sequencing (NGS) data for the academic and translational research ... Unit, and will continue to focus on development of ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy ... has an expanded team of Regional Pharmaceutical Care ... prescriber offices in their regions, with specific areas ... a variety of Regional Pharmaceutical Care Liaisons specializing ... hepatitis C help prescribers understand all the new ...
Breaking Biology Technology:Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Bina Technologies is acquired by Roche. 2Bina Technologies is acquired by Roche. 3BioPlus Specialty Pharmacy Expands ‘Regional Pharmaceutical Care Liaison’ Team 2BioPlus Specialty Pharmacy Expands ‘Regional Pharmaceutical Care Liaison’ Team 3
... 11 Pressure,BioSciences, Inc. (Nasdaq: PBIO ... it has entered into a Cooperative Research ... Army Medical Research Institute of,Infectious Diseases (USAMRIID). ... use of the Company,s patented pressure cycling ...
... and safety of food supply, as well as increasing concerns ... to experience spiraling growth given the recent success of commercialized ... pipeline. , ... San Jose, California ...
... Update on Key Regulatory Milestones, SEATTLE, Sept. 11 ... MTA: CTIC) announced today that it will host an,investor ... investor day will,provide an update from executive management on ... meeting will be an,invitation-only event. A webcast and live ...
Cached Biology Technology:Pressure BioSciences, Inc. Announces Research Agreement With the US Army Medical Research Institute of Infectious Diseases (USAMRIID) 2Pressure BioSciences, Inc. Announces Research Agreement With the US Army Medical Research Institute of Infectious Diseases (USAMRIID) 3Pressure BioSciences, Inc. Announces Research Agreement With the US Army Medical Research Institute of Infectious Diseases (USAMRIID) 4Pressure BioSciences, Inc. Announces Research Agreement With the US Army Medical Research Institute of Infectious Diseases (USAMRIID) 5Long-Term Investments Remain Critical to Biotechnology Industry Growth, According to a New Report by Global Industry Analysts 2Long-Term Investments Remain Critical to Biotechnology Industry Growth, According to a New Report by Global Industry Analysts 3Long-Term Investments Remain Critical to Biotechnology Industry Growth, According to a New Report by Global Industry Analysts 4
(Date:12/11/2014)... N.C. , Dec. 10, 2014  That blood pressure ... quite a while. Hypertension – the medical term for high ... the early 1800s, and the inflatable cuff that,s used in ... doesn,t, however, mean there,s nothing new about hypertension, its triggers ... long-held beliefs about the condition and the best ways to ...
(Date:12/10/2014)... -- You,ve been here before: you desperately need to sign ... site key or the answer to your secret question. What,s ... Today, Hoyos Labs , a digital infrastructure ... put an end to the frustration that comes with usernames, ... leverages a user,s smartphone to acquire his or her biometrics ...
(Date:12/10/2014)... , Dec. 9, 2014  Wake Forest Baptist Medical Center ... for its School of Medicine. Funding for this $50 ... campaign that will be publicly launched next summer. ... in the former 60 series R.J. Reynolds Tobacco Company ... Construction will begin immediately with plans to be ready ...
Breaking Biology News(10 mins):Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4
... honeybee hives arrests the growth of prostate cancer cells and ... at the University of Chicago Medicine. Caffeic acid phenethyl ... propolis, the resin used by bees to patch up holes ... natural remedy for conditions ranging from sore throats and allergies ...
... Biology Reports published today argues that recent advances ... of the global demand for liquid transportation fuels, drastically reducing ... burning fossil fuels, without having an impact on food production. ... of detrimental climate change is the emission of greenhouse gasses ...
... brain activity in the rat brain that play a ... decision-making. The discovery, which builds on the team,s previous ... post-traumatic stress syndrome. The researchers previously identified patterns ... region critical for memory storage. The patterns sometimes represented ...
Cached Biology News:Beehive extract shows potential as prostate cancer treatment 2Beehive extract shows potential as prostate cancer treatment 3Better plants for biofuels 2Rats recall past to make daily decisions 2Rats recall past to make daily decisions 3
... convenient, and efficient native protein concentration following ... spin column to make handling easy. The ... which were electro-eluted in their native condition. ... high binding capacity fo all pH values. ...
... optically clear polystyrene with frosted labeling ... for cell counting. 100% integrity tested. ... new product number, created to easily ... no availability yet, please order under ...
... The Corning CellBIND surface is produced ... a more hydrophilic surface giving more consistent, ... yields. CellBIND enhances cell attachment and growth ... serum-free medium. • CellBIND may provide a ...
... system, 100-240 V, uses xMAP technology for ... proteins and peptides in one microplate well. ... to 100 color-coded bead sets, each of ... reactant (enzyme substrates, receptors, antigens, or antibodies) ...
Biology Products: