Navigation Links
Selection of Standards for Bio-Plex Cytokine Assays, Rev A

ng the following formula: (observed concentration) / (expected concentration) x 100. If the regression analysis is accurate, the calculated concentrations will be very close to the actual concentrations of the standards. This method yields information about the dynamic range, sensitivity, and accuracy of calculated concentrations of an assay (Baud 1993). The recovery value for each standard is an indication of the accuracy of the calculated concentration. A value within the range of 70130% is considered accurate recovery. The dynamic range of the assay, or the range where accurate concentrations may be quantitated, is therefore indicated by the range of standards yielding a recovery of 70130%.

A comparison of the standards recovery using broad range and high-sensitivity standards for three different cytokines is shown in Tables 2 and 3. The broad range standard concentrations where recovery is 70130% is 1.95 to 8,000 or 32,000 pg/ml, depending on the cytokine assayed. The high-sensitivity standards yield a narrower dynamic range of 0.2 or 0.78 to 3,200 pg/ml. In all three assays, the broad range standards enable the quantitation of a much wider range of concentrations.

A general indication of assay sensitivity is given by the lowest concentration in a set of standards that yields accurate recovery (70130%). Since the lowest concentration of the high-sensitivity standards is lower than the broad range standards (i.e., 0.2 pg/ml vs. 1.95 pg/ml, respectively), it would be expected that the high-sensitivity standards would yield better recovery at a lower concentration, and that the high-sensitivity standards should be chosen to maximize sensitivity in an assay. Tables 2 and 3 illustr
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Plasmid Miniprep Kit Selection
3. Universal Guide to DNA Standards
4. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
5. Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
6. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
7. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
8. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
9. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
10. Simultaneous Detection of 8 Cytokines in Mouse or Human Sera
11. The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Selection Standards for Bio Plex Cytokine Assays Rev

(Date:7/11/2014)... , July 11, 2014 EPFL ... announce the appointment of John P. Donoghue . ...   in  Geneva . The new ... Biotech in Geneva , has named ... neuroscientists. John P. Donoghue , founder of Brown ...
(Date:7/10/2014)... 10, 2014 Product and process ... low abundance and are often “lost in the ... , Join presenters Dr. Rowel Tobias, Senior Scientist, ... Anders, Head of Quality at Nanotherapeutics, Inc., to ... can speed detection and quantitation while achieving the ...
(Date:7/10/2014)... Poway, CA (PRWEB) July 10, 2014 Robert ... Regenerative Veterinary Medicine company, is proud to announce the relaunch ... Pets, launching with a new series called “ What are ... to give an honest and straightforward foundation in the basics ... an educated decision on the right type of treatment when ...
(Date:7/10/2014)... , July 10, 2014 ... Dmitry Medvedev , presented Russia,s ... BIOCAD . The Company is developing a unique ... MabNext project BIOCAD develops a number of innovative drugs ... autoimmune diseases. The ceremony took place at the International ...
Breaking Biology Technology:John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 2John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 3Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2
... Wis. - After months of debate and restructuring Gov. ... - at the expense of the Biomedical Technology Alliance ... exercising a partial veto on a proposed grant of $2.5 ... investors located in southeastern Wisconsin. The partial veto will cut ...
... BIO 2005 meeting in Philadelphia last month, the globalization of ... flight. The company that perhaps best chronicles and tracks this ... biotechnology. , ,This report is usually released and previewed at ... appropriate title Beyond Borders: Global Biotechnology Report 2005, is a ...
... two-year state budget signed into law Monday by Gov. ... Wisconsin technology firms by changing how corporate income from ... Technology Council. , ,With bipartisan support from the ... factor" sales apportionment for corporate income to technology and ...
Cached Biology Technology:Doyle partially vetoes SE Wisconsin technology grant 2Doyle partially vetoes SE Wisconsin technology grant 3Globalization of biotech industry is a decidedly worldwide phenomenon 2Globalization of biotech industry is a decidedly worldwide phenomenon 3`Single-sales factor' extends tax treatment to tech, service firms 2`Single-sales factor' extends tax treatment to tech, service firms 3
(Date:7/11/2014)... Researchers from Ume University, Sweden, have explored two ... the reaction sequence leading to the formation of ... been published in the scientific journal Nature ... improving present day synthetic catalysts for water oxidation, ... devices for the direct storage of solar energy ...
(Date:7/11/2014)... the normal circumstances, astrocytes participate in normal ... and exhibit therapeutic and repairing effects on ... have found that nerve cells differentiated from ... reduced viability, which produces influences on subsequent ... General Hospital, Hebei United University, China demonstrated ...
(Date:7/10/2014)... and other major cities in New Mexico, nearly every ... wastewater rather than precious potable water supplies. Across the ... all golf courses receive treated effluent. Reusing the effluent ... golf courses and homeowners alike fertilize their lawns during ... nitrate. A New Mexico State University turfgrass expert has ...
Breaking Biology News(10 mins):Molecular snapshots of oxygen formation in photosynthesis 2'Tailored' water -- the latest in lawn care 2
... to find the necessities of life. It,s a complex task, ... environment to navigate the safest and fastest routes to food ... certain important events, such as finding a meal, will occur. ... fundamental behaviors, navigation and the anticipation of a reward, had ...
... /PRNewswire-iReach/ -- IdentiSys Inc., North America,s ... emergency response, and access control security systems, today announced ... in Customer and Patient Experience Management systems. IdentiSys will ... to the marketplace and will be the only North ...
... re-growing tropical forests has concluded that plant biodiversity takes ... such as clearance for farming. The findings, ... Royal Society B , have important implications for conservation ... and Central America. The new study is the first ...
Cached Biology News:Anticipation and navigation: Do your legs know what your tongue is doing? 2Anticipation and navigation: Do your legs know what your tongue is doing? 3Anticipation and navigation: Do your legs know what your tongue is doing? 4IdentiSys brings Qmatic Q-Guide Queuing Systems to the North American market 2IdentiSys brings Qmatic Q-Guide Queuing Systems to the North American market 3Carbon storage recovers faster than plant biodiversity in re-growing tropical forests 2
... DNA Kit enables efficient extraction of DNA fragments from ... agarose gels. The procedure uses GelMelt solution to dissolve ... to a silica membrane in a spin column format. ... in low-salt buffer. Each spin column can bind up ...
... efficient extraction of DNA from agarose gels The ... fragments of 150 bp to > 12,000 bp in ... to dissolve the gel slice, followed by adsorption of ... column format. After a wash step, the purified DNA ...
When added to either StemSep CD4+ or CD8+ T Cell Enrichment cocktails, human HLA-DR TAC is designed to deplete human HLA-DR from samples to isolate resting CD4+ or CD8+ T cells....
BlotGel application pool -see Blotters (Semi-Dry) for more info...
Biology Products: