Navigation Links
Screening of Beer-Spoilage Bacteria Using,,,the LightCycler PCR Workflow System

PCR Workflow System for analyzing beer routine samples.

Sensitivity and specificity

Table 1 shows that the LightCycler foodproof Beer Screening Kit detects all beer-spoilage bacteria species.



The kit allows the detection of beer-spoilage bacteria from as little as 102 cfu/ml. In some cases, such as L. brevis and Megasphaera, even the highest dilution with 101 cfu/ml can be detected. Except for Pediococcus, all tested spoilage bacteria were detectable in the 102 cfu/ml dilution and in higher concentrations. In the case of Pediococcus, a Pediococcus species was identified that is not a beer-spoilage organism.

Melting-curve analysis

Figure 1 gives an example for the most frequent beerspoilage bacteria: L. brevis. For all four different cell concentrations the typical melting peak for L. brevis at 59.5C is clearly visible and allows the easy identification of this very troublesome bacterium. These results show that the LightCycler foodproof Beer Screening Kit allows the detection of beer-spoilage bacteria with high sensitivity and specificity.



The results of the melting-curve analysis of 20 different DNA samples of the most important beer-spoilage bacteria are presented in Table 2. Out of the 20 samples tested, 19 were identifed correctly. Pediococcus claussenii belongs to the group of L. plantarum and L. coryniformis; thus, this result also has to be regarded as correct. Mixed DNA samples containing up to four different species were also identified. The high conformity shows the wide specificity of the LightCycler foodproof Beer Screening Kit and confirms its suitability for the user.

'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. LightCycler foodproof Beer Screening Kit
3. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
4. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
5. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
6. A Mix-and-Read Cell-Based Assay for Hybridoma Screening Using the FMAT 8100 HTS System
7. Simultaneous Screening of 23 Drugs of Abuse in Oral Fluid Using an LC/MS/MS Method
8. Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS
9. Screening of Corticosteroids in Urine by Negative Atmospheric Pressure Chemical Ionization LC/MS/MS
10. Screening and classifying hazardous waste samples
11. High-Throughput Cultivation of Bacterial Clones
Post Your Comments:
(Date:7/10/2014)... Robert Harman, DVM, Founder and CEO of Vet-Stem, Inc., ... the relaunch of his highly informative blog, now named Stem ... What are Stem Cells ?” Dr. Harman’s purpose for ... the basics of stem cell therapy so that pet owners ... treatment when considering regenerative medicine. , A veterinarian by trade, ...
(Date:7/10/2014)... EvoDerma ’s NOOME Anti-Aging Motion has been ... comes with a second treatment cup to help prevent and ... cup is thinner on the edges for a softer contact ... and uneven surfaces on the face, neck and décolleté. Choose ... on areas such as the cheeks and neck, or opt ...
(Date:7/10/2014)... POINT, Ind. , July 10, 2014 ... expanded its use of capnography for respiratory ... growing group of healthcare leaders in embracing ... measures how effectively patients are breathing and ... depression occurs. By measuring the amount of ...
(Date:7/10/2014)... , July 10, 2014  Kainos Capital, a ... brands, today announced that it has acquired the Slim-Fast ... in the business. Terms of the transaction were not ... and meal replacement business that markets ready-to-drink shakes, powders, ... North America and in the ...
Breaking Biology Technology:Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3Introducing a New Bonus Treatment Cup for EvoDerma’s NOOME Anti-Aging Motion 2Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2Kainos Capital Acquires Slim-Fast From Unilever 2
... The Beaumont Technology,Usability Center (BTUC) will offer ... technology companies speed their product commercialization,process. ... BTUC product-usability and,human-factors experts to conduct a ... risk assessment requirements. In early 2008, the,organizations ...
... focus on Next Generation Sequence Analysis Problem -, ... Genomics Search Tool -, MISSION VIEJO, Calif., ... community for its innovative genomic search,technology, SLIM Search, ... Institute,s (CHI) Second International Next Generation,Sequencing Conference. The ...
... Calif., Sept. 26 Jazz,Pharmaceuticals, Inc. (Nasdaq: ... Executive Chairman, will provide a corporate presentation at ... NY on October 8,2007 at 8:00 a.m. Eastern ... may be accessed by visiting the,Investors section of ...
Cached Biology Technology:Beaumont Technology Usability Center To Offer Training For Medical Technology Companies 2SLIM Search, Inc. Sponsors Next Generation Sequencing Conference for the Biomedical Market 2SLIM Search, Inc. Sponsors Next Generation Sequencing Conference for the Biomedical Market 3SLIM Search, Inc. Sponsors Next Generation Sequencing Conference for the Biomedical Market 4
(Date:7/11/2014)... German . ... the refraction of X-rays through a specimen instead of ... method are often of much higher quality than those ... Prof. Franz Pfeiffer are particularly interested in developing new ... X-ray phase-contrast imaging. One main goal is to make ...
(Date:7/11/2014)... Shenzhen, July 10, 2014---A team of researchers from The ... have identified a gene of wild soybean linked to ... to grow in saline soil. This study published online ... to unveil novel genomic information for crop improvement. , ... to domestication and human selection, cultivated soybeans have less ...
(Date:7/11/2014)... An international team of researchers, including scientists from the ... just a reported a major step in understanding photosynthesis, ... now maintains the oxygen in its atmosphere and which ... on earth. , The researchers report the first ... reaction, namely the step in which a specific protein ...
Breaking Biology News(10 mins):New simple setup for X-ray phase contrast 2BGI reports a novel gene for salt tolerance found in wild soybean 2A first direct glimpse of photosynthesis in action 2
... environmental engineer has received a three-year, $561,730 grant to identify ... water providers can take action to contain and curb the ... Foundation have awarded Assistant Professor Hyeok Choi the grant to ... exist so that the harmful algae can be monitored. , ...
... mouth are telling Oak Ridge National Laboratory scientists something ... code of bacteria linked to the condition. The ... of Sciences , profiles the SR1 bacteria, a group ... mouth to deep within the Earth, that have never ...
... the most widely advocated strategies for dealing with HIV/AIDS could ... of men who have sex with men (MSM) in LA ... In the United States, LA County has the largest ... and treat" policy which calls for universal testing for ...
Cached Biology News:UT Arlington engineer to search for bad algal blooms 2Human microbe study provides insight into health, disease 2Study: Widespread 'test-and-treat' HIV policies could increase dangerous drug resistance 2
...
...
... System is an imaging based plate ... and high throughput screening which can ... The Hamamatsu light sources and cameras ... decades-long experience in photon detection technology. ...
... pCITE-2a-c(+) vectors are designed for enhanced translation of ... e.g. rabbit reticulocyte lysate. The maps for pCITE-2b(+) ... the following exceptions: pCITE-2b(+) is a 3793 bp ... I and Xcm I sites. Subtract 7 bp ...
Biology Products: