( PCR Workflow System for analyzing beer ...)Screening of Beer-Spoilage Bacteria Using,,,the LightCycler PCR Workflow System

PCR Workflow System for analyzing beer routine samples.

Sensitivity and specificity

Table 1 shows that the LightCycler foodproof Beer Screening Kit detects all beer-spoilage bacteria species.



The kit allows the detection of beer-spoilage bacteria from as little as 102 cfu/ml. In some cases, such as L. brevis and Megasphaera, even the highest dilution with 101 cfu/ml can be detected. Except for Pediococcus, all tested spoilage bacteria were detectable in the 102 cfu/ml dilution and in higher concentrations. In the case of Pediococcus, a Pediococcus species was identified that is not a beer-spoilage organism.

Melting-curve analysis

Figure 1 gives an example for the most frequent beerspoilage bacteria: L. brevis. For all four different cell concentrations the typical melting peak for L. brevis at 59.5C is clearly visible and allows the easy identification of this very troublesome bacterium. These results show that the LightCycler foodproof Beer Screening Kit allows the detection of beer-spoilage bacteria with high sensitivity and specificity.



The results of the melting-curve analysis of 20 different DNA samples of the most important beer-spoilage bacteria are presented in Table 2. Out of the 20 samples tested, 19 were identifed correctly. Pediococcus claussenii belongs to the group of L. plantarum and L. coryniformis; thus, this result also has to be regarded as correct. Mixed DNA samples containing up to four different species were also identified. The high conformity shows the wide specificity of the LightCycler foodproof Beer Screening Kit and confirms its suitability for the user.

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