Navigation Links
Screening of Beer-Spoilage Bacteria Using,,,the LightCycler PCR Workflow System

rification quality by Magnetic Bead Technology
  • Improved reproducibility by precise pipetting steps

  • Sample names were directly imported with a bar-code reader and transferred via network from the MagNA Pure Instrument to the LightCycler Instrument. This minimized the risk of wrong entries and further reduced the hands-on time. Utilizing the MagNA Pure Total NA Large Volume protocol meant that the enriched samples could be directly used for nucleic acid purification without any additional centrifugation steps. For sample preparation, 1 ml of enrichment culture was predispensed into the wells of the MagNA Pure Sample Cartridge. Following fully automated nucleic acid purification, DNA was eluted in 50 l final volume. The complete PCR mixes were then set up automatically. The MagNA Pure LC Instrument compiled and loaded the PCR mixes directly into LightCycler Capillaries in the LightCycler Carousel.

    PCR setup

    For one reaction of 20 l final volume, 13 l LightCycler foodproof Beer Screening Mastermix, 1 l LightCycler foodproof Beer Screening Enzyme Solution and 1 l LightCycler foodproof Beer Screening Internal Control were automatically mixed and pipetted together with 5 l sample DNA into each LightCycler Capillary.

    Suitability testing of routine samples

    58 routine samples including control, different kinds of beer, fermenter, rinsing water and other yeast-containing samples were tested. To assess the efficiency of the MagNA Pure LC System in removing PCR inhibitors, the purified DNA was analyzed with the LightCycler foodproof Beer Screening Kit.

    Sensitivity and specificity testing of dilution series

    '"/>

    Source:


    Page: All 1 2 3 4 5

    Related biology technology :

    1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
    2. LightCycler foodproof Beer Screening Kit
    3. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
    4. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
    5. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
    6. A Mix-and-Read Cell-Based Assay for Hybridoma Screening Using the FMAT 8100 HTS System
    7. Simultaneous Screening of 23 Drugs of Abuse in Oral Fluid Using an LC/MS/MS Method
    8. Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS
    9. Screening of Corticosteroids in Urine by Negative Atmospheric Pressure Chemical Ionization LC/MS/MS
    10. Screening and classifying hazardous waste samples
    11. High-Throughput Cultivation of Bacterial Clones
    Post Your Comments:
    (Date:8/20/2014)... Stimulating nerves in your ear could improve the health ... at the University of Leeds used a standard TENS ... apply electrical pulses to the tragus, the small raised ... front of the ear canal. , The stimulation changed ... by reducing the nervous signals that can drive failing ...
    (Date:8/20/2014)... Sofia, Bulgaria (PRWEB) August 20, 2014 ... was released today including improvements to the ... rates and connectors for Lucene, SOLR and Elasticsearch. ... change for this high performance triplestore – GraphDB™ ... organizations interested in deploying the only mature enterprise ...
    (Date:8/19/2014)... (PRWEB) August 19, 2014 Shimadzu ... chromatography systems, Prominence-i and Nexera-i, adding to the ... Combining excellent functionality, an intuitive operating environment, and ... a more efficient workflow for conventional to ultra-high-speed ... innovative, intuitive and intelligent design so users can ...
    (Date:8/19/2014)... , Aug. 19, 2014 A Geisinger researcher ... nominated for a Centers for Disease Control and Prevention ... major public health concern. Joseph Boscarino ... one of a team of researchers nominated for the ... Shepard Science Award for Data Methods and Study Design. ...
    Breaking Biology Technology:'Tickling' your ear could be good for your heart 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 3Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 4Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 2Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 3Geisinger investigator nominated for federal award 2Geisinger investigator nominated for federal award 3
    ... YORK, March 21, 2011 /PRNewswire-Asia-FirstCall/ -- American Oriental Bioengineering, ... "AOBO"), a pharmaceutical company dedicated to improving health through ... of prescription and over the counter ("OTC") products, announced ... 15, 2010, that its board of directors authorized a ...
    ... Md., March 21, 2011 United Therapeutics Corporation ... enrollment in its FREEDOM-C(2) registration trial was completed ... a 16-week, multi-center, international, double-blind, randomized, placebo-controlled study ... diethanolamine (UT-15C) in pulmonary arterial hypertension (PAH) patients ...
    ... Recent events in Japan have caused the world to ... be a cause for concern – especially if radiation ... proliferating cells of the body. Cells of the hematopoietic ... two such cell types. Damage to these cells initiates ...
    Cached Biology Technology:American Oriental Bioengineering Announces US$20 Million Share Repurchase Program 2United Therapeutics Corporation Completes Enrollment of FREEDOM-C(2) Trial 2Existing Radiation Countermeasures Inadequate! Development of Rx100 Promises Change! 2Existing Radiation Countermeasures Inadequate! Development of Rx100 Promises Change! 3
    (Date:8/20/2014)... Bay Area Lyme Foundation, which aims to make Lyme ... new research published in an upcoming issue of the ... . The findings show that ticks that carry ... year, making the threat of Lyme disease year-round. The ... Public Health (CDPH) Vector-borne Disease Section and University of ...
    (Date:8/20/2014)... suffered concussions ready to return to action? A new ... athletes who head back on the field with medical ... their abilities to simultaneously walk and do simple mental ... their balance and/or altered walking speed, was found in ... returned to activity in less than a month. Seven ...
    (Date:8/20/2014)... During the past few years, Virginia Tech,s Wu Feng ... Microsoft grant from the "Computing in the Cloud" program, ... a $6 million award from the Air Force on ... from NSF and the National Institutes of Health on ... wove together the "parallel computing" aspects from each grant, ...
    Breaking Biology News(10 mins):Lyme disease risk is year-round in Northwest California, according to new study 2Lyme disease risk is year-round in Northwest California, according to new study 3Recovery reversal seen in Oregon study of returning concussed athletes 2Recovery reversal seen in Oregon study of returning concussed athletes 3The internet was delivered to the masses; parallel computing is not far behind 2The internet was delivered to the masses; parallel computing is not far behind 3The internet was delivered to the masses; parallel computing is not far behind 4
    ... Even healthy pregnant women can be at risk for ... Reserve University began to understand which bacteria from the 700 ... health problem of preterm and stillbirths. Yiping Han ... Dental Medicine led the study, which found several new bacteria ...
    ... laboratories, two Dartmouth biologists have performed one of the largest ... population. Their results, published online on May 9 by the ... more important than predation by birds and snakes when it ... Tennyson wrote that nature is ,red in tooth and claw, ...
    ... May 7, 2010 In findings that advance scientists, ... from The Scripps Research Institute has shed light on a ... other lysosomal storage diseases. The findings were published in ... Biology on May 9, 2010. "This study is ...
    Cached Biology News:Even healthy pregnant women need to worry about oral bacteria 2Researchers use entire islands in the Bahamas to test survival of the fittest 2Scripps research scientists shed light on potential treatment for Gaucher's disease 2Scripps research scientists shed light on potential treatment for Gaucher's disease 3Scripps research scientists shed light on potential treatment for Gaucher's disease 4
    Human monocyte-derived dendritic cells, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Dendritic Cells and EPCs...
    ...
    ...
    ...
    Biology Products: