( ...)Screening of Beer-Spoilage Bacteria Using,,,the LightCycler PCR Workflow System

The new LightCycler foodproof Beer Screening Kit (dedicated to the detection of beer-spoilage bacteria on the LightCycler Instrument) in combination with the MagNA Pure LC DNA III Kit (for the automated isolation of bacterial DNA on the MagNA Pure LC Instrument) offers breweries an innovative and automated solution for monitoring contamination of beer. This PCR Workflow System enables breweries to test their product consistency in less than two hours.

The LightCycler foodproof Beer Screening Kit includes all reagents necessary for the amplification and the detection of beer-spoilage bacteria in a single reaction. The kit detects 22 of the most relevant species that belong to the genera Lactobacillus, Pediococcus, Pectinatus, and Megasphaera. In addition, the LightCycler foodproof Beer Screening Kit allows the identification of the most important beer-spoilage bacteria via melting-curve analysis.

The Bitburger Brewery checked the suitability of the PCR Workflow System using different routine brewery samples. Additionally, Bitburger assessed the sensitivity and reliability of the melting-curve analysis in identifying beer-spoilage bacteria.

Materials and Methods

Sample preparation

Sample preparation (nucleic acid isolation) and postelution steps (PCR setup) were carried out on the MagNA Pure LC Instrument using the modified MagNA Pure DNA Isolation Kit III (Proteinase K dissolved in 6 ml Elution Buffer). Advantages compared with a manual workflow are:

• Significantly less hands-on-time
• Reduced risk of cross-contamination
• Highest pu
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