Navigation Links
Screening and classifying hazardous waste samples


Introduction
Timely and accurate analysis of hazardous materials is fundamental to environmental protection. In addition to the obvious necessity of identifying a suspected contaminant, a detailed analytical profile can help trace the source of a dangerous material and contribute to selecting the best containment and cleanup strategy.

A substantial body of research has clearly established the efficiency and economy of fluorescence spectroscopy as a technique for characterizing many hazardous substances. One recent effort in this area involved the use of a SPEX FLUOROLOG spectrofluorometer for detecting, classifying, and quantifying samples containing petroleum oils or chemicals like polychlorinated biphenyls (PCBs) and aromatics.

The project was conceived and carried out by US Army Corps of Engineers affiliates DeLyle Eastwood and Russell Lidberg. Their aim was to enlarge the use of spectroscopic pattern recognition for environmental projects conducted by the Department of Defense under the Defense Environmental Restoration Account. The two researchers successfully applied fluorescence, low-temperature luminescence, and, for comparative purposes, Fourier-transform infrared spectroscopy to the analysis of hazardous waste samples for sites in Alaska and Kansas. Their work was also intended to augment the library of spectral references available for analysis of petroleum oils and fluorescent hazardous chemicals.

Eastwood and Lidberg used standard emission methods as well as excitation and synchronous scanning techniques at both room and liquid-nitrogen temperatures. With appropriate reference standards and emission methodology, samples can be quantified over a range from 100 ppb to a few ppb, even where extraction is from difficult matrices such as river sediments. The experimental results compiled by Eastwood and Lidberg emphasize how spectrofluorometry can easily accomplish what is much harder to achieve via conventional approaches using gas chromatography and mass spectrometry.


Experimental Procedures
Computerized search routines for classifying spectra based on feature sets and similarity measures were developed. For fluorescence, pattern recognition factors encompassed spectral area, peak positions, and angular distance between spectra.

For all fluorescence spectra, The FLUOROLOG research spectrofluorometer system included a single-grating excitation monochromator and a double-grating emission monochromator. The excitation source was a 150-W xenon lamp. Both emission and reference detectors contained photomultiplier tubes.

Twenty-nine reference oil samples were obtained from the Environmental Protection Agency and the U.S. Coast Guard Research and Development Center. These references were chosen to be representative of the principal types of petroleum oils: light fuels, heavy fuels, and crude oils. Standard solutions were prepared from the reference oils at a concentration of 20 g/g in cyclohexene. Reference solutions for polychlorinated biphenyl (PCB) analysis were prepared by dilution to concentrations of 1030 g/g in cyclohexene. Real-world samples obtained for analysis were divided into three groups: contaminated soil samples, neat samples that appeared to be pure oils, and liquid samples (many of which contained a water phase) that were not pure oils.

All spectra presented herein were taken with the excitation bandpass at 4.5 nm, and emission band-pass at 0.9 nm. Excitation was at 254 nm.


Results and Discussion
The 29 reference oils were characterized by their emission spectra as well as by data obtained from synchronous scanning. Eastwood and Lidberg tabulated all of this information, categorizing the results of their experiments according to spectral features like maximum peak wavelengths, wavelengths for distinctive secondary peaks and shoulders, and the relative areas under corrected spectral curves.


Figure 1 shows emission spectra for a typical No. 2 fuel oil and a typical No. 6 fuel oil. The differences are clear, permitting easy classification. The differences between two No. 6 oils illustrated in Figure 2, however, are slight. Furthermore, in Figure 3, note that the Prudhoe Bay crude oil spectrum is very similar to the data shown in Figure 2. Such close correspondence in emission spectra is common, and can lead to classification errors.



When emission data are not sufficient for conclusive sample identification, synchronous scanning can provide significantly more useful spectral structure. Synchronous scanning entails simultaneous scanning of the excitation and emission monochromators with a constant offset between them. The recorded intensity is proportional to the product of the observed excitation and emission intensities. Accordingly, a significant difference could then be discerned between the maximum peak wavelengths of the No. 6 fuel oils in Figure 4 and the Prudhoe Bay crude oil in Figure 5. Whereas the maximum peak positions were identical for the samples emission spectra, synchronous scanning increases the difference in position by around 50 nm.



Figures 6, 7, and 8 provide practical examples comparing emission spectra of unknown oil samples and library references. Figure 6 shows that an analytical sample extracted from soil yields recognizable and useful spectra. Figure 7 reveals a close correspondence between a known Prudhoe Bay crude oil and an unknown sample received in isopropanol. Figure 8 is interesting because it compares a weathered real-world sample with a known JP-4 jet fuel. Fluorescence analysis of samples weathered in a thin film or water is less common because of potential data distortion. From research, the correct analytical approach reveals distinguishable spectra even after periods of weathering ranging from two days to several weeks.




With regard to hazardous chemicals, a 1979 Coast Guard report lists approximately 90 substances that can be readily identified by their room-temperature fluorescence spectra. Eastwood and Lidberg indicated that this list could be extended to about 250 hazardous materials if low-temperature luminescence/phosphorescence spectra were included.

Figure 9 shows a spectral comparison of PCB samples acquired at 77 K, where the emission intensity is greater for phosphorescence than fluorescence. Low-temperature analysis is considerably more sensitive, allowing quantification in the ppb range. For field-screening in the ppm range, room-temperature fluorescence appears satisfactory, for fluorescence quenching by the internal heavy-atom effect is incomplete.


In the future, Eastwood and Lidberg intend to use their SPEX system to expand the library of spectral references for petroleum oils and hazardous chemicals. The exceptional sensitivity of their FLUOROLOG system also will be applied to developing a more comprehensive system of classifying spectra: one that would encompass subclasses based on spectral characterization rather than just API categories or physical properties. Information about the geographical origin of oils could also be integrated into such a framework.

In addition, preliminary PCB measurements need to be expanded and refined to ensure better detection, identification, and quantification for these ubiquitous chemicals, especially in field work. The same holds true for other important classes of fluorescing hazardous chemicals, among them organochlorine pesticides, dioxins, and polynuclear aromatic molecules.


back to top
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Screening of Beer-Spoilage Bacteria Using the LightCycler PCR Workflow System
3. LightCycler foodproof Beer Screening Kit
4. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
5. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
6. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
7. A Mix-and-Read Cell-Based Assay for Hybridoma Screening Using the FMAT 8100 HTS System
8. Simultaneous Screening of 23 Drugs of Abuse in Oral Fluid Using an LC/MS/MS Method
9. Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS
10. Screening of Corticosteroids in Urine by Negative Atmospheric Pressure Chemical Ionization LC/MS/MS
11. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Screening and classifying hazardous waste samples

(Date:12/24/2014)... PharmaBoardroom,s new report, , ... available for free download , digs deep into this ... in the sector today. One area where ... in developing a homegrown pharmaceutical manufacturing base, even if the ... some way off. A cursory comparison with neighboring ...
(Date:12/22/2014)... Md. , Dec. 22, 2014 /PRNewswire/ ... SYN), a developer of pathogen-specific therapies for ... on protecting the microbiome, today announced positive ... Phase 1a clinical trial of SYN-004, the ... prevention of Clostridium difficile (C. difficile) ...
(Date:12/22/2014)... The American Journal of Hematology/Oncology, ... and editorials addressing developments and pathways related to ... provocative article exploring the role of follicle-stimulating hormone ... of prostate cancer. , The article references ... that there could be a connection to prostate ...
(Date:12/19/2014)... 2014 BioPlus Specialty Pharmacy ... the promotion of Nick Maroulis, Pharm.D. to the newly ... , In this position, Dr. Maroulis will continue ... the directors of our multi-site pharmacies as the company ... and during that time he has served in many ...
Breaking Biology Technology:PharmaBoardroom Releases New Algeria Pharmaceuticals Report 2PharmaBoardroom Releases New Algeria Pharmaceuticals Report 3Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 2Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 3Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 4Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 5Prostate Cancer Experts Dissect the Role of Follicle-stimulating Hormone in the Development, Progression and Potential Treatment of Prostate Cancer 2Prostate Cancer Experts Dissect the Role of Follicle-stimulating Hormone in the Development, Progression and Potential Treatment of Prostate Cancer 3Prostate Cancer Experts Dissect the Role of Follicle-stimulating Hormone in the Development, Progression and Potential Treatment of Prostate Cancer 4BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2
... ... Ingredients for Follow-on Biologics in Chicago. , ... Chicago (Vocus) April 22, 2010 -- Therapeutic Proteins Inc. ... (or biosimilars) manufacturing facility constructed to meet U.S. Food and Drug Administration (FDA) requirements. ...
... iCardiac ... , ... (PRWEB) April 22, 2010 -- iCardiac Technologies, Inc., a provider of next generation cardiac ... a cardiac safety study leveraging iCardiac’s COMPAS technology platform including advanced ECG biomarker analyses., ...
... Promega Corporation now ... whole blood. , ... 21, 2010 -- Promega Corporation now offers the Maxwell® 16 LEV Blood DNA Kit ... work on the Maxwell® 16 instrument, eliminates the need for a buffy coat step while ...
Cached Biology Technology:Therapeutic Proteins Inc. Completes First Independent, Dedicated Biosimilars Manufacturing Facility in U.S. 2Therapeutic Proteins Inc. Completes First Independent, Dedicated Biosimilars Manufacturing Facility in U.S. 3Therapeutic Proteins Inc. Completes First Independent, Dedicated Biosimilars Manufacturing Facility in U.S. 4Another Top 10 Pharmaceutical Company Awards Cardiac Safety Study to iCardiac 2New Promega Maxwell 16 LEV Blood DNA Kit Surpasses Concentration Standards in Less Time 2
(Date:12/17/2014)... Research and Markets ( http://www.researchandmarkets.com/research/s6qzt5/samsung_galaxy_s5 ... "Samsung Galaxy S5 - Home Button Synaptics Fingerprint ... http://photos.prnewswire.com/prnh/20130307/600769 ... than the iPhone 5S, Samsung introduces for the ... The Galaxy S5 home button presents ...
(Date:12/17/2014)... 16, 2014 Research and Markets ... the "Global Chemical Sensor Market 2015-2019" ... http://photos.prnewswire.com/prnh/20130307/600769 One major trend ... for medical sensors in biomedical applications. Chemical sensors ... and correct diagnosis during surgical procedures. The Global ...
(Date:12/11/2014)... , Dec. 09, 2014 Research and ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has ... in India 2015-2019" report to their offering. ... this market is the adoption of multimodal biometric ... characteristic of an individual for verification and identification ...
Breaking Biology News(10 mins):Samsung Galaxy S5 - Home Button Synaptics Fingerprint Sensor Technology Analysis 2Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... climate changes over the past several decades have led ... squirrels, birds and mosquitoes, according to University of Oregon ... the June 9 issue of SCIENCE, William E. Bradshaw, ... show that diverse animal populations have changed genetically in ...
... University of Florida scientists conducting experiments with mice have ... of cells in the eye essential for healthy vision. ... cell layer vital for eyesight called the retinal pigment ... writing in a recent issue of Investigative Ophthalmology & ...
... of human autoimmune disorders and other diseases works by dialing ... production, University of Michigan researchers have found. , "Many drugs ... Gary Glick, who is the Werner E. Bachmann Collegiate Professor ... volume control, so we're able to dial enzyme activity down ...
Cached Biology News:Recent, rapid climate change is driving evolution of animal species 2Bone marrow may restore cells lost in vision diseases 2Bone marrow may restore cells lost in vision diseases 3Drug dials down the energy within cells, UM researchers find 2
... Ready-to-use liquid controls for monitoring ... (normal & abnormal) with targeted manufacturing ... and 20 ± 3 IU/L. ... stored at 2-8 C. Level 1: ...
EDTA, 0.5 M solution, 100 ml. Ethylene diaminetetraacetic acid.Sterile, nuclease-free. Category: Nucleotides & Enzymes & Biochemicals, Ultrapure Biochemicals ....
Request Info...
Recombinant Mouse sTNF RII/TNFRSF1B...
Biology Products: