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Sciclone ALH Bulk Dispense Precision Testing

ispensed twice into a waste reservoir then secondary dispensed into each column of a NUNC (Denmark) 96 well, clear bottom, untreated plate with the Bulk Dispenser (Fig.1). Deionized/degassed water was then dispensed into each well to provide a final volume of 200μL (Fig.2). Absorbance was measured and the resulting data exported to Excel for CV determination. The entire process was repeated 5 times for each volume of tartrazine dispensed.

Results

Utilizing the programs depicted in Fig.1 and Fig.2, five plates of each volume (80, 40, 20 and 10μL) were prepared, absorbance measured and the data exported to ExcelTM For CV determination (Table 1).

Discussion

In this study, we demonstrate the high level of precision that can be expected of the Sciclone ALH Bulk Reagent Dispenser. This device permits non-contact delivery of 10-2500μL volumes to specified columns or all columns of multiwell plates (secondary dispensing). Moreover, serial dilutions are easily formatted because the volume dispensed is selectively variable for any column.

The Sciclone ALH can accommodate up to six (Four if configured with the Z8 option) bulk reagent dispensers each with its own fluid path using either a syringe pump (this study) or a peristaltic pump. Our results clearly demonstrate that the bulk reagent dispenser can easily dispense a wide range of volumes with an exceptional degree of precision.


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