Navigation Links
Sciclone ALH Bulk Dispense Precision Testing

Jeff Cunningham and Lynn Jordan
Caliper Life Sciences Inc., Applications Laboratory, Hopkinton, Massachusetts, USA

Introduction

The ability to continuously dispense reagents across multi-well plates and into individual columns (secondary dispensing) is of great importance to high throughput laboratories. Equally important, however, is the precision that can be achieved during a series of dispenses. Therefore, the bulk reagent dispensing performance of the Sciclone Advanced Liquid Handler Workstation was tested for precision. Using the absorbance properties of various tartrazine solutions, we found average variation coefficients (CV's) of <1%, 1%, 1.2% and 2.2% for volumes of 80, 40, 20 and 10μL respectively. Our data exemplifies the capabilities of the Bulk Reagent Dispenser at various microliter ranges.

Materials and Methods

Instrumentation
All testing was performed using a Sciclone Advanced Liquid Handler (ALH) and a Bulk Reagent Dispenser (Sciclone pipetting head option). Absorbance was measured at 450nm with a Wallac Victor2 1420 Multilabel Counter (PerkinElmer Wellesley, MA).

Reagents
Tartrazine (SIGMA) solutions (1.25, 1, 0.16 and 0.08g/L) corresponding to 10, 20, 40 and 80μL dispenses were prepared with deionized H2O and degassed with Helium.

Priming
To facilitate the removal of any compressible gasses, at least three 2.5mL volumes of system fluid (in this case degassed tartrazine) are primed into a waste reservoir. Then, manual methods are used to force residual air pockets that collect at the top of the syringes into the lines by three cycles of additional priming.

Precision Determination
Tartrazine volumes (10, 20, 40 or 80μL) were d
'"/>

Source:


Page: All 1 2

Related biology technology :

1. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
2. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
3. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
4. Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
5. Dispensing 0.5μL to "Dry" 384 Plates with the Sciclone ALH
6. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
7. Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
8. Chemical Stability of the Brinkmann Bottletop Dispenser and the Brinkmann Bottletop Buret
9. Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)
10. 96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation
11. Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Sciclone ALH Bulk Dispense Precision Testing

(Date:6/29/2015)... ... June 30, 2015 , ... This cutting edge standard ... machines. Featuring 12 distinct peaks, the WAV-8 is the world’s first cuvette-shaped, solid-state ... spec deep into the NIR range. Previous spectrophotometer calibration standards, such as those made ...
(Date:6/29/2015)... , June 29, 2015  AACC, a global ... health through laboratory medicine, is pleased to announce ... Clinical Chemistry , has increased to 7.9 in ... increase places Clinical Chemistry in the ... the significant influence of the research it publishes ...
(Date:6/29/2015)... June 29, 2015   Veracyte, Inc . (Nasdaq: ... field of molecular cytology, today announced that it has ... Top Workplaces list for the second year in a ... solely on an annual survey of Bay Area-company employees. ... the degree to which Veracyte values our employees and ...
(Date:6/26/2015)... , June 26, 2015 /PRNewswire/ - iCo Therapeutics ("iCo" ... announced that all nominees listed in the management ... at its 2015 Annual Meeting of Shareholders, held ... by ballot, the following 5 nominees proposed by ... to serve until the Company,s next Annual Meeting ...
Breaking Biology Technology:FireflySci Releases Spectrophotometer Calibration Standards for NIR Wavelength Accuracy Verification 2FireflySci Releases Spectrophotometer Calibration Standards for NIR Wavelength Accuracy Verification 3AACC's Journal, Clinical Chemistry, Continues to Have Significant Impact on Medical Testing, Healthcare 2AACC's Journal, Clinical Chemistry, Continues to Have Significant Impact on Medical Testing, Healthcare 3Veracyte Named a 2015 "Top Workplace" in the San Francisco Bay Area 2Veracyte Named a 2015 "Top Workplace" in the San Francisco Bay Area 3iCo Therapeutics Announces Voting & Election Results from Annual Meeting of Shareholders 2iCo Therapeutics Announces Voting & Election Results from Annual Meeting of Shareholders 3
... Nov. 12, 2010 Ardea Biosciences, Inc. (Nasdaq: ... and chief executive officer, will present at the following upcoming ... Healthcare Conference in New York, NY. Presentation: 1:40 p.m. EST on ... Annual Health Care Conference in New York, NY. Presentation: 1:00 ...
... from Thomson Reuters released today shows that the US is no ... kept pace with the increased output from European and Asia-Pacific nations. ... found that research output from the US has been surpassed by ... 40 percent of research papers in the most influential journals(1). By ...
... Cat fanciers everywhere appreciate the gravity-defying grace and exquisite balance ... extend even to the way cats lap milk? Researchers ... domestic and big cats lap and found that felines of ... physical forces. The results will be published in the ...
Cached Biology Technology:Ardea Biosciences to Present at Two Upcoming Investor Conferences 2Ardea Biosciences to Present at Two Upcoming Investor Conferences 3US Dominance in Scientific Research Significantly Challenged by Increased Competition from Europe and Asia, According to Thomson Reuters Study 2Cats show perfect balance even in their lapping 2Cats show perfect balance even in their lapping 3Cats show perfect balance even in their lapping 4
(Date:6/24/2015)... YORK , June 24, 2015 The ... rate. The major drivers for the market include growth ... number of new drug launches. Over the years, the ... increased. Between 2001 and 2010, the FDA approved 225 ... according to WIPO, Europe accounted ...
(Date:6/23/2015)... 23, 2015   MedNet Solutions , an ... entire spectrum of clinical research, is pleased to ... , the company,s intuitive, flexible and affordable cloud-based ... 2015 Stevie® Award by the American Business ... Website category.  The American Business Awards are considered ...
(Date:6/18/2015)... June 18, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... on the growing mobile commerce market announces that its Wocket® ... on "Money on the Mark", scheduled to air on WABC ... June 20 th . The broadcast air- ... 7:00 to 8:00pm EST. NXT-ID, Inc.,s CEO Gino Pereira ...
Breaking Biology News(10 mins):Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 2Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 3Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 4MedNet Solutions' iMedNet eClinical Wins Silver Stevie Award 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 3
... challenges Africa faces when it comes to feeding its ... and famine let alone broader food security issues ... these challenges in the longer term is equally important, ... and development. A re-invigorated effort to boost African ...
... Protection Agency issued its first comprehensive report on major ... list, accounting for more than 70 percent of industrial ... Many climate experts have urged power plants and other ... atmosphere and sequester it deep underground a technique ...
... Energy (DOE),s Lawrence Berkeley National Laboratory (Berkeley Lab) have ... protein (CETP) mediates the transfer of cholesterol from "good" ... (LDLs). These findings point the way to the design ... could help prevent the development of heart disease. ...
Cached Biology News:Cutting-edge science creating solutions for African agriculture 2Stanford scientist to discuss the challenges and opportunities of carbon sequestration at AAAS 2How good cholesterol turns bad 2How good cholesterol turns bad 3
... number is a new product ... Cornings product number. If showing ... under the old Sigma-Aldrich number ... for assistance. Mfr Desig: ...
... Virgin, optically clear polystyrene with frosted ... coordinates for cell counting. 100% integrity ... a new product number, created to ... showing no availability yet, please order ...
... LIC Duet™ T7•Tag ® Ek Adaptor ... operator, ribosome binding site (rbs), ATG translation ... sequence. The T7•Tag is a small epitope ... detection of the target protein with T7•Tag ...
The LIC Duet™ Mini Adaptor encodes a T7 promoter, lac operator, ribosome binding site (rbs), and an ATG translation initation codon. This adaptor is designed for expression of a target protei...
Biology Products: