Navigation Links
Sciclone ALH Bulk Dispense Precision Testing

Jeff Cunningham and Lynn Jordan
Caliper Life Sciences Inc., Applications Laboratory, Hopkinton, Massachusetts, USA

Introduction

The ability to continuously dispense reagents across multi-well plates and into individual columns (secondary dispensing) is of great importance to high throughput laboratories. Equally important, however, is the precision that can be achieved during a series of dispenses. Therefore, the bulk reagent dispensing performance of the Sciclone Advanced Liquid Handler Workstation was tested for precision. Using the absorbance properties of various tartrazine solutions, we found average variation coefficients (CV's) of <1%, 1%, 1.2% and 2.2% for volumes of 80, 40, 20 and 10μL respectively. Our data exemplifies the capabilities of the Bulk Reagent Dispenser at various microliter ranges.

Materials and Methods

Instrumentation
All testing was performed using a Sciclone Advanced Liquid Handler (ALH) and a Bulk Reagent Dispenser (Sciclone pipetting head option). Absorbance was measured at 450nm with a Wallac Victor2 1420 Multilabel Counter (PerkinElmer Wellesley, MA).

Reagents
Tartrazine (SIGMA) solutions (1.25, 1, 0.16 and 0.08g/L) corresponding to 10, 20, 40 and 80μL dispenses were prepared with deionized H2O and degassed with Helium.

Priming
To facilitate the removal of any compressible gasses, at least three 2.5mL volumes of system fluid (in this case degassed tartrazine) are primed into a waste reservoir. Then, manual methods are used to force residual air pockets that collect at the top of the syringes into the lines by three cycles of additional priming.

Precision Determination
Tartrazine volumes (10, 20, 40 or 80μL) were d
'"/>

Source:


Page: All 1 2

Related biology technology :

1. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
2. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
3. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
4. Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
5. Dispensing 0.5μL to "Dry" 384 Plates with the Sciclone ALH
6. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
7. Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
8. Chemical Stability of the Brinkmann Bottletop Dispenser and the Brinkmann Bottletop Buret
9. Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)
10. 96 Well Pipetting Precision and Accuracy Using the RapidPlate 96/384 Workstation
11. Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Sciclone ALH Bulk Dispense Precision Testing

(Date:5/1/2015)... May 1, 2015 /PRNewswire/ - BioAmber Inc. (NYSE: ... announced that it has priced an underwritten registered offering ... price of $9.00 per share, and granted the underwriters ... to an additional 585,000 shares of its common stock. ... million.  The offering is expected to close on May 6, ...
(Date:5/1/2015)... 1, 2015 /CNW/ - Softchoice, a leading North American IT solutions ... proud recipient of two Cisco® Partner Summit Country awards. ... Year and Security Partner of the Year for ... this week in Montreal . ... Cisco partner awards at this year,s conference," said David ...
(Date:5/1/2015)... LONDON , May 1, 2015 ... following equities: Genetic Technologies Ltd (NASDAQ: GENE ... Thermo Fisher Scientific Inc. (NYSE: TMO ), ... Inc. (NYSE: PKI ). Free research report ... http://get.Investor-Edge.com/pdf/?c=Genetic%20Technologies&d=01-May-2015&s=GENE . On Thursday, April 30, 2015, the ...
(Date:5/1/2015)... , May 1, 2015 ... Production Market by Product (Equipment, Consumables), by Type (Monoclonal, ... Diagnostic Labs, Research Institutes) - Analysis & Global ... studies the global Antibody Production Market for the ... is expected to reach $2.572 Billion by 2019 ...
Breaking Biology Technology:BioAmber Inc. Announces Pricing Of $35.1 Million Registered Offering 2BioAmber Inc. Announces Pricing Of $35.1 Million Registered Offering 3Softchoice recognized with two Country Awards at Cisco Partner Summit 2015 2Life Sciences Tools and Services Stocks on our Radar -- Genetic Technologies, Agilent Technologies, Thermo Fisher Scientific, Sequenom, and PerkinElmer 2Life Sciences Tools and Services Stocks on our Radar -- Genetic Technologies, Agilent Technologies, Thermo Fisher Scientific, Sequenom, and PerkinElmer 3Life Sciences Tools and Services Stocks on our Radar -- Genetic Technologies, Agilent Technologies, Thermo Fisher Scientific, Sequenom, and PerkinElmer 4Life Sciences Tools and Services Stocks on our Radar -- Genetic Technologies, Agilent Technologies, Thermo Fisher Scientific, Sequenom, and PerkinElmer 5Antibody Production Market worth $2.572 Billion by 2019 2Antibody Production Market worth $2.572 Billion by 2019 3Antibody Production Market worth $2.572 Billion by 2019 4
... For NDA and to Complete Current Phase 3 Trial ... BETHESDA, Md., June 23 Spherix Incorporated (Nasdaq: ... therapy, and a provider of technical and regulatory consulting ... announced that it has received its first full-scale production ...
... Diagnostic Industry Magazine,s 30 Years, 30 Devices Special Issue Commends B. ... Medical Device and Diagnostic Industry has recognized three medical devices ... infusion therapy and pain management as well as a pioneer in ... the most influential medical devices of the last 30 years. ...
... Human Protein Atlas, available exclusively through Sigma-Aldrich, bring the portfolio ... , ST. LOUIS, June 23 Sigma-Aldrich (Nasdaq: ... additions to its Prestige Antibodies(R) powered by Atlas Antibodies product ... of highly validated antibodies to over 6,100. Developed by the ...
Cached Biology Technology:Spherix Announces Full-Scale Production of Pharmaceutical Tagatose 2Spherix Announces Full-Scale Production of Pharmaceutical Tagatose 3Spherix Announces Full-Scale Production of Pharmaceutical Tagatose 4B. Braun Medical Inc. Recognized by Industry for Manufacturing Influential Medical Devices 2B. Braun Medical Inc. Recognized by Industry for Manufacturing Influential Medical Devices 3B. Braun Medical Inc. Recognized by Industry for Manufacturing Influential Medical Devices 4Sigma-Aldrich and Atlas Antibodies Announce 2,300 New, Highly Validated Prestige Antibodies(RM) 2Sigma-Aldrich and Atlas Antibodies Announce 2,300 New, Highly Validated Prestige Antibodies(RM) 3Sigma-Aldrich and Atlas Antibodies Announce 2,300 New, Highly Validated Prestige Antibodies(RM) 4
(Date:4/9/2015)... SAN JOSE, Calif. , April 9, 2015 /PRNewswire/ ... of human interface solutions, announced today that it will ... 2015 on Thursday, April 23, 2015, after the close ... call for analysts and investors at 2:00 p.m. PT ... information.    To participate on the live ...
(Date:4/2/2015)... 2015 At its 2015 ACMG Annual Clinical ... , the American College of Medical Genetics and Genomics ... its Board.  Members of the ACMG Board of Directors ... and advancing its policies and programs. ACMG is the ... "It,s an eventful time in medical genetics and genomics.  ...
(Date:4/1/2015)... --  Medisafe ™, the leading global medication management platform ... and Android smartphones and tablets, announces today ... to allow patients to track and correlate their adherence ... will be able to visualize in real-time how taking ... as glucose levels and blood pressure. "Patients ...
Breaking Biology News(10 mins):Synaptics to Report Third Quarter Results on April 23 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8Medisafe Announces Launch of Biometric Integration, Enabling Patients to Stay Adherent to Their Meds and See Tangible Health Results 2
... study has found that some bird species in the desert ... by widespread fire through their habitat. In fact, the Baylor ... of the habitat that is formed after a fire is ... that three specific bird species in the Chihuahuan Desert ...
... NC The shape of a hummingbird,s beak allows for a ... in a mere fraction of a second with greater speed and ... new study in a forthcoming issue of Journal of Theoretical ... but hummingbirds can,t live on nectar alone. To get enough protein ...
... new journal in systematic and biology of fungi (including lichens) was launched ... Dr Thorsten Lumbsch from The Field Museum, Chicago assisted by an authoritative ... is free to read, download, print, and distribute. MycoKeys ... and PhytoKeys . It is more than ...
Cached Biology News:Study finds some desert birds less affected by wildfires and climate change 2Hummingbirds catch flying bugs with the help of fast-closing beaks 2Pensoft Publishers launch a new mycological open-access journal 2Pensoft Publishers launch a new mycological open-access journal 3
... is a new product number, ... product number. If showing no ... the old Sigma-Aldrich number (R3776) ... assistance. Material: cap polystyrene ...
BD Falcon 850 cm Smooth-surface TufRol Roller Bottle, tissue-culture treated polystyrene, blow-molded one-piece design, with easy on/off non-vented cap, packed in double bag. (20/ca) Packaging: ...
... Ion Trap Time of Flight Mass Analyzer for superior sensitivity, mass ... 100 - 25,000 m/z, * Precursor ion selection range: 150 - ... accuracy: 5 ppm with internal reference, * Dynamic range: 4 orders ... ...
... Vector is specifically designed to target and ... Saccharomyces cerevisiae. Displayed proteins can be analyzed ... or putative ligands. pYD1 uses the a-agglutinin ... domains, Aga1 and Aga2, to display recombinant ...
Biology Products: