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Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing

ffect on the precision performance of a given system, it is likely that the calibration curves used in the liquid class will differ depending on which method is used. It has been found in this and other work that exiting the sample slowly after a wet transfer pro - duces a cleaner breakoff at the tip or cannula, and can have a significant impact on precision performance.

Conclusions:

Since the physical properties of the sample carries more variability than the HVH device at low volumes, the choice of liquid or solution for critical low volume work will have a significant impact. To obtain optimal results when working with samples that are viscous, or have poor wetting properties, the geometry and materials of the tip should also be considered3.

Acknowledgements:

The authors would like to thank Jim O'Keefe for his initial efforts and advice in beginning this work.

1 Niven, R.W. et al. 1996. Protein Nebulization. 2. Int. J. Pharm. 127 iss. 2 pp. 191-201.

2 Massignon, D. 1987. Science des Interfaces, Mouillabilit, Adhsion et Frottement. Synthse de la Runion et Prospective. Vers une Approche des Problmes Industriels. J. Chim. Phys. PCB. 84 no.2 pp.135-140.

3 Barton, A. 1982. The Application of Cohesion Parameters to Wetting and Adhesion- A Review. J. Adhesion. 14 pp.33-62.


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