Navigation Links
Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing

/p>

Results:

For the gravimetrically determined dispensing of the Sciclone HVH, weights dispensed were corrected for evaporation. The magnitude of evaporation over the 90 second experimental time frame can be seen for all sample types in Figure 2: A and B.

Since this is a wet transfer and there is sample in the plate at the start of the experiment, an evaporation rate of >100% at 1 μL is possible. During the course of our experiments, with water taken as the "standard" sample type, we see that most samples, with the exception of Ethanol and DMSO, did not have evaporation rates that varied by more than 2-3% from water (Fig. 3).

In addition to the %CV data generated, it was also noted that the behavior of BSA solution was not ideal under the experimental conditions used here. At 20 μL/sec as an aspiration rate, small bubbles formed. Although this did not affect the precision greatly, the surface tension properties of bubbles are known to damage proteins1.

Discussion:

At pipetting volumes >5 μL, plate-to-plate %CV's attained for the Sciclone 3000 ALH were <1%. In the 1-2 μL range, a CV of <2.5% is possible with the proper pipetting technique. The addition of a surfactant (Triton X-100) dramatically improved the precision, possibly due to better wetting properties2 . This is particularly clear at 1 μL, where the CV is close to 1%. Since different liquids accountant for more than a 4% change in CV (at 1 μL), this demonstrates that variability in sample properties introduces more error into pipetting protocols than is inherent in the precision of the Sciclone ALH High Volume Head device (<3% CV). These results indicate that slight modifications to sample solutions can yield better pipetting results. Although the method for compensating for or eliminating backlash will not have an e
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Sciclone ALH Bulk Dispense Precision Testing
2. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
3. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
4. Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
5. Dispensing 0.5μL to "Dry" 384 Plates with the Sciclone ALH
6. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
7. Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
8. Ergonomics and Liquid Handling How can BrinkmannTM Help ?
9. Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
10. Serial Dilution with the Bravo Liquid Handling Platform
11. Epitope-Tagging Vectors for Functional Analysis in Yeast
Post Your Comments:
(Date:1/22/2015)... / Rootstown, OH (PRWEB) January 22, 2015 ... platform, announced today that it has received AOAC-PTM Certifications for ... O1O3, O111, O121, and O145; collectively referred to as STEC ... O157, at 1 colony forming unit (cfu) per 325 g ...
(Date:1/22/2015)... (PRWEB) January 22, 2015 zFlo Motion ... pleased to announce an exciting product from a new partner. ... analysis solutions over the past 15 years, with offerings in ... recently, their 3D IMU system (inertial measurement unit), iSen, is ...
(Date:1/22/2015)... Controlled Substance Compliance Services (CSCS), a ... check the legal requirements around using controlled substances in ... , As their international operations expand and become increasingly ... built as a result of the first phase of ...
(Date:1/22/2015)... Denville, NJ (PRWEB) January 22, 2015 ... shearing technologies such as the Bioruptor® and complete ... semi-automation system for chromatin immunoprecipitation, alleviating the need ... reagents and controls needed for ChIP of histones ...
Breaking Biology Technology:Crystal Diagnostics Awarded AOAC-PTM Accreditation for the Rapid Detection of “Big 6” E.coli Food Pathogens 23D Motion Capture Just Got Easy 2Global Compliance Service for Controlled Substances to Expand to China 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2
... 2 Randomized Study Under Consideration, ROCKVILLE, Md., ... a clinical-stage pharmaceutical company developing,therapeutics for the treatment ... has met the primary endpoint for the efficacy ... MKC-1 in combination with pemetrexed,(Alimta(R)) in non-small cell ...
... NANJING, China, Nov. 10 /Xinhua-PRNewswire/ -- Simcere Pharmaceutical,Group ... supplier of branded generic,pharmaceuticals and innovative anti-cancer pharmaceuticals ... participation in the Morgan,Stanley 7th Annual Asia Pacific ... Mandarin Oriental Singapore on November 11-13, 2008., ...
... Partners will provide partnership for identifying expansion and investment opportunities. ... ... (PRWEB) November 10, 2008 -- World Trade Center Dulles Airport ... seeking to access global markets, today announced its business launch ...
Cached Biology Technology:EntreMed's MKC-1 Meets Primary Efficacy Endpoint in Non-Small Cell Lung Cancer Clinical Trial 2EntreMed's MKC-1 Meets Primary Efficacy Endpoint in Non-Small Cell Lung Cancer Clinical Trial 3Simcere Pharmaceutical Group to Participate in Morgan Stanley Seventh Annual Asia Pacific Summit 2008 2Former NASDAQ International President Among Execs Launching Firm to Access Global Markets 2Former NASDAQ International President Among Execs Launching Firm to Access Global Markets 3
(Date:12/17/2014)... Valencell, a leader in performance biometric data sensor technology, ... such as Intel, Jabra, Atlas, and Scosche to develop ... products will be showcased at CES 2015, taking place ... "Our partners choose PerformTek biometrics, because ...
(Date:12/11/2014)... Markets , http://photos.prnewswire.com/prnh/20130307/600769 ... addition of the "Biometrics Market in India 2015-2019" ... major trend emerging in this market is the adoption ... than one characteristic of an individual for verification and ...
(Date:12/10/2014)...  Wake Forest Baptist Medical Center today announced plans for a ... for this $50 million capital project is part of a ... The medical education building will be located ... adjacent to 525@vine in Wake Forest Innovation Quarter. Construction will ...
Breaking Biology News(10 mins):Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4
... oyster native to the West Coast could face a double ... research from the University of California, Davis, Bodega Marine Laboratory. ... Proceedings of the Royal Society B . Invasive snails ... snails were raised under ocean conditions forecast for the end ...
... Minn. , Jan. 15, 2014 MedNet Solutions ... study management systems, is pleased to announce that the latest ... released to the iMedNet user community.  iMedNet ... eClinical platform ideally suited to Sponsors and CROs wanting to ...
... news release is available in German . ... economists and politicians usually call a win-win situation: a relationship between ... acacia plants and the ants that live on them is an ... of food bodies and nectar as well as hollow thorns which ...
Cached Biology News:Acidification, predators pose double threat to oysters 2MedNet Solutions Announces Latest Release Of Its Innovative iMedNet EDC eClinical Solution 2Ants protect acacia plants against pathogens 2Ants protect acacia plants against pathogens 3
Biotin d anti-mouse Tim-1...
Mouse monoclonal [MLM544] to cardiac Myosin light chain 1 ( Abpromise for all tested applications). entrezGeneID: 4634 SwissProtID: P08590...
Anti-20S proteasome subunits alpha 1, 2, 3, 5, 6 & 7, clone MCP231 Immunogen: Dinitrophenylated proteasomes Molecular Weight: 29 and 32 kDa....
Anti-ADP-Ribosylation Factor 1, clone 3F1 Immunogen: Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1. Available Date: 38495...
Biology Products: