Navigation Links
Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing

bserved due to differences in plate brand.

Reservoir:
A HyperTask Grooved Reservoir (P/N RES03384) was used for all sample types.

Density and evaporation rates were determined gravimetrically using a Sartorius BP211 D analytical balance. Evaporation rates were measured daily, by dispensing 100 μL of the sample into a plate, and recording the weight change in time. Day to day variation in the evaporation rates were found to be as much as 1.5% for a 1 μL water sample.

Pipetting Technique:
The liquid class used for all of this work ran at 20 μL/sec rate, with no air-gap and a linear curve assigned for the full range. The backlash for this HVH device (<5 μL) was determined visually, by aspirating 1 μL increments until liquid entered the tips.

Experimental Protocol:

All machine movements were at 100% travel speed except during the first 2 dispense steps, where a 5% retraction travel speed was used. At each sampling point (1, 2, 5, 10, 100, and 180 μL) excess sample (>5 μL) was aspirated from the reservoir. Some sample was then dispensed back to the reservoir (>5 μL) to eliminate the backlash.

To standardize the evaporation rate for each data point, data acquisition times were measured with a VWR laboratory timer. For the 180 μL sampling point, a dry plate was weighed and placed on the Sciclone deck. The timer was started when the sample began dispensing into a dry plate, and the second weight was taken 90 seconds later. For all other sampling points, the plate started wet, with at least 60 μL of sample in it. The timer was started when the first weight was taken, the plate was placed on the Sciclone deck, the protocol was run, and the plate was removed and placed on the balance. The second weight was recorded 90 seconds after the first.<
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Sciclone ALH Bulk Dispense Precision Testing
2. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
3. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
4. Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
5. Dispensing 0.5μL to "Dry" 384 Plates with the Sciclone ALH
6. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
7. Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
8. Ergonomics and Liquid Handling How can BrinkmannTM Help ?
9. Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
10. Serial Dilution with the Bravo Liquid Handling Platform
11. Epitope-Tagging Vectors for Functional Analysis in Yeast
Post Your Comments:
(Date:7/30/2015)... -- HIGHLIGHTS:Q2 2015 Results (all changes are ... sales were $697 million compared to $701 million in ... 8%, and changes in foreign currency exchange rates decreased ... , By business unit, organic sales growth was ... SAFC Commercial. , Reported diluted EPS was $0.98 ...
(Date:7/30/2015)... ... July 30, 2015 , ... GEA's Pony™ NS2006L ... operation up to 1500 bar. The sanitary design gauge of the NS2006L is ... , The Pony™ NS2006L homogenizer is an ideal solution for the processing of ...
(Date:7/29/2015)... , July 30, 2015 According ... Market - Growth, Trends & Forecasts (2015-2020), , published by ... US$ 30.25 billion by the end of 2020, with ... accounting for more than 40% of the global market size. ... at a CAGR of 13.7% during the period of (2015-2020). ...
(Date:7/29/2015)... /PRNewswire/ - BioAmber Inc. (NYSE: BIOA ) today announced ... Officer has resigned.  The Company has hired Andrew ... in December 2014, as full time interim CFO and ... Andy Ashworth began his employment today, ... transition.  Andy was the Company,s CFO from September 2011 ...
Breaking Biology Technology:Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 2Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 3Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 4Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 5Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 6Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 7Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 8Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 9Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 10Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 11Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 12Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 13Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 14Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 15Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 16Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 17Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 18Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 19Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 20GEA Announces the Pony™ NS2006L… a Self-contained, High Pressure Lab Homogenizer for Product Development Ensuring Maximum Efficiency and Safety 2Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 2Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 3BioAmber Announces Change in Chief Financial Officer 2BioAmber Announces Change in Chief Financial Officer 3
... In the latest development in an ongoing global legal ... a New York court against Harvey Boulter, the CEO of investment ... in New York follows a decision earlier this month by a ... Porton Group,s Washington DC attorney was invalid. 3M,s withdrawal ...
... 29, 2012  Dyadic International, Inc. ("Dyadic") (OTC Pink: ... discovery, development, manufacture and sale of enzyme and ... biopharmaceutical industries, announced today that it has entered ... a leading global provider of nutritional ingredient solutions ...
... to detect and respond to magnetic fields is not ... some bacteria and various migratory animals, do respond to ... turtles, this behavior involves small magnetic particles in the ... they are actually doing is not fully understood. ...
Cached Biology Technology:3M Withdraws Blackmail Claims Against Harvey Boulter From New York Court 2Dyadic International Partners with Leading Provider of Nutritional Ingredient Solutions for Production of Food Enzymes 2Dyadic International Partners with Leading Provider of Nutritional Ingredient Solutions for Production of Food Enzymes 3The laws of attraction: Making magnetic yeast 2The laws of attraction: Making magnetic yeast 3
(Date:7/23/2015)... July 23, 2015 Aware, Inc. (NASDAQ: AWRE ... reported financial results for its second quarter ended June 30, ... 2015 was $4.5 million, a decrease of 33% compared to ... in the second quarter of 2015 was $0.3 million, or ... $0.04 per diluted share, in the same period a year ...
(Date:7/21/2015)... YORK , July 21, 2015 ... systems, as seen in the recent U.S. Office ... a solution to the infosec conundrum, but developers ... is stored. To address this dilemma, Biometrics-as-a-Service provider ... its Biometric Tokenization SDK for third party integration. ...
(Date:7/9/2015)... , July 9, 2015  Synaptics Inc. (NASDAQ: ... announced today that it will report financial results for ... July 30, 2015 after the close of market. The ... and investors at 2:00 p.m. PT (5:00 p.m. ET), ... To participate on the live call, analysts and ...
Breaking Biology News(10 mins):Aware, Inc. Reports Second Quarter 2015 Financial Results 2Aware, Inc. Reports Second Quarter 2015 Financial Results 3Aware, Inc. Reports Second Quarter 2015 Financial Results 4Aware, Inc. Reports Second Quarter 2015 Financial Results 5Aware, Inc. Reports Second Quarter 2015 Financial Results 6HYPR Corp. Launches Industry First Biometric Tokenization Platform 2HYPR Corp. Launches Industry First Biometric Tokenization Platform 3HYPR Corp. Launches Industry First Biometric Tokenization Platform 4Synaptics to Report Fourth Quarter, Fiscal Year 2015 Results on July 30 2
... new map of the potato genome that may lead ... help feed the world,s hungry. By sequencing and ... consortium has, for the first time, tied specific potato ... and development of potatoes may enable scientists and breeders ...
... Institute, one of the National Institutes of Health, has ... of Medicine each approximately $2.3 million a year for ... Glycosciences. Gerald Hart, Ph.D., director of biological chemistry and ... will lead these independent efforts to better understand the ...
... TORONTO, Ont., July 12, 2001--Eating nuts every day could ... according to new research from St. Michael,s Hospital and ... online by the journal Diabetes Care , a ... of Toronto Department of Nutritional Sciences; St. Michael,s Hospital ...
Cached Biology News:Johns Hopkins researchers awarded $32 million 2A 'nutty' solution to type 2 diabetes management 2
... up to 6 strain chambers with precise uniaxial ... design ensures that cells grow over long periods ... This unit allows you to control the frequency ... unique strain settings. The instrument fits in a ...
... Quantas unique combination of Coulter volume ... capabilities and accurate cell counting. This ... including UV, allowing flexible fluorochrome selection ... typically only achievable with larger, more ...
... The Cytomics FC 500 Series ... equipped with the most advanced ... Series delivers flexible testing, powerful ... features that help researchers optimize ...
... antibody irreversibly crosslinked to protein G-agarose beads ... complex from heart mitochondria. Also included are ... control immunocapture. ,The pyruvate dehydrogenase complex (PDH) ... small amounts of tissue. This facilitates the ...
Biology Products: