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Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing

s used. For all movements other than these retractions, the Sciclone ALH was run at 100% travel speed.

Finally, the issue of evaporation must be addressed. Although this is an accuracy, rather than a precision issue, variations in accounting for evaporation rates interfere with the precision results at the 1-2 μL data points. Therefore, all data are corrected for evaporation. Evaporation varies depending on environmental conditions; however, the magnitude of evaporation is reported to give the researcher a general guideline as to what to expect, and perhaps when it may be necessary to take evaporation into account.

Materials and Methods

All experiments were run using a Sciclone ALH 3000 workstation with software version 3.1.14, firmware version 1.14 and a High Volume Head (S/N SS0309N4928).

Liquids evaluated in this study include:


Purified Water [Culligan System]
BupH Modified Dulbecco's Phosphate Buffered Saline Packs [Pierce No. 28374]
Ethanol [J.T. Baker A-478-09]
Dimethyl Sulfoxide (DMSO) [J.T. Baker 9224-01]
1 mg/mL Bovine Serum Albumin (BSA) [Sigma A-7906] solution in purified water
10% Glycerol [ICN Biomedical, Inc. No. 806688] in purified water
1% Triton TX-100 [Sigma A-7906] in purified water.

Pipette Tips:
CaliperLS Automation Certified TempoTM-100 and TempoTM-200 tips were used for all experiments. Fresh tips were used for each sample. Tempo-200 tips were only used for the 180 μL dispenses while the Tempo-100 tips were used for all other data points.

Plates: A variety of untreated 96-well, flat-bottomed, polystyrene plates were used. No evidence for differences in sample delivery were o
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