selectable assignable pumps for acid,
base, nutrient addition or harvesting as well as foam control, and is PC -compatible for simplified data logging, monitoring and
bioprocess control. For production of secreted products from mammalian cells, the patented immobilized-cell basket accessory
( Figure 3 ) enables production rates of 1 gram of MAb/day in as little as 5 L of culture medium2
. Researchers facing the
challenges of scaling up should be aware that introductory information is available to ease their transition, including papers on
fermentor and bioreactor selection and operation.3-5
1. G. Wang, W. Zhang, Y. Chen, C. Jacklin, H. Song, and D. Freedman.
Design and Performance of a Packed-Bed Bioreactor for the Production of
Recombinant Protein Using Serum-Free Medium. Edison, NJ: New
Brunswick Scientific, 1994.
2. J. Doyle, L. Johnstone and L. Cottis. Gram Quantities of MAb Produced
with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic
Fluid Production. AGEN Biomedical Ltd., Queensland, Australia.
Published in September 1996 Biomass News edition of New Brunswick
3. J. Cino PhD and S. Frey. 20 Tips for Purchasing Research Fermentors
and Bioreactors. Biopharm Sept 1996 & Feb 1997 (reprints available from
New Brunswick Scientific.
4. Fundamentals of Fermentation Techniques for Benchtop Fermentors.
Edison, NJ: New Brunswick Scientific, 1995.
5. Selected References to Scientific Research in Fermentation and Cell
Culture. Edison, NJ: New Brunswick Scientific, 1998.
Page: All 1 2 3 4 5 6 Related biology technology :1
Mr. Julien is Fermentation and Cell Culture Product Manager,
New Brunswick Scientific Co., Inc., 44 Talmadge Rd., P.O. Box 4005,
Edison, NJ 08818-4005, USA; Tel.: 800-631-5417, 732 -287-1200;
. A technique for increasing yields in bioreactors and disposable cell-culture systems2
. Improving the accuracy and speed of mammalian cell counting3
. Easy preparation of total RNA from cultured cells with TRIzol and
Eppendorf Phase Lock Gel4
. Embryonic stem cells, mouse5
. Primary cells, rat heart endothel6
. Primary cells, rat heart muscle7
. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN
siRNA and TransMessenger Transfection Reagent*8
. Flow cytometric analysis of human
primary cells using the Agilent 2100
bioanalyzer and on-chip staining9
. 2100 expert software
Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer10
. Sensitive detection of tumor cells in
peripheral blood of carcinoma patients
by a reverse transcription PCR method11
. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells