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Scaling-Up from Spinners, T-Flasks & Shakers: A versatile bioreactor for mammalian and microbial cells

selectable assignable pumps for acid, base, nutrient addition or harvesting as well as foam control, and is PC -compatible for simplified data logging, monitoring and bioprocess control. For production of secreted products from mammalian cells, the patented immobilized-cell basket accessory ( Figure 3 ) enables production rates of 1 gram of MAb/day in as little as 5 L of culture medium2. Researchers facing the challenges of scaling up should be aware that introductory information is available to ease their transition, including papers on fermentor and bioreactor selection and operation.3-5


1. G. Wang, W. Zhang, Y. Chen, C. Jacklin, H. Song, and D. Freedman. Design and Performance of a Packed-Bed Bioreactor for the Production of Recombinant Protein Using Serum-Free Medium. Edison, NJ: New Brunswick Scientific, 1994.

2. J. Doyle, L. Johnstone and L. Cottis. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production. AGEN Biomedical Ltd., Queensland, Australia. Published in September 1996 Biomass News edition of New Brunswick Scientific newsletter.

3. J. Cino PhD and S. Frey. 20 Tips for Purchasing Research Fermentors and Bioreactors. Biopharm Sept 1996 & Feb 1997 (reprints available from New Brunswick Scientific.

4. Fundamentals of Fermentation Techniques for Benchtop Fermentors. Edison, NJ: New Brunswick Scientific, 1995.

5. Selected References to Scientific Research in Fermentation and Cell Culture. Edison, NJ: New Brunswick Scientific, 1998.


Mr. Julien is Fermentation and Cell Culture Product Manager, New Brunswick Scientific Co., Inc., 44 Talmadge Rd., P.O. Box 4005, Edison, NJ 08818-4005, USA; Tel.: 800-631-5417, 732 -287-1200; F
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