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Scaling-Up from Spinners, T-Flasks & Shakers: A versatile bioreactor for mammalian and microbial cells

n mammalian culture rarely exceeds 150 rpm. Additionally, specialized low-shear impellers, such as marine blade, pitched blade, or Cell LiftTM (New Brunswick Scientific, Edison, NJ) impellers are required. The patented Cell Lift impeller allows for direct sparging of large amounts of gas into the medium through the impellers cell-free aeration cage. Cells and microcarriers are too large to pass through the pores of the screen and are therefore protected from the hazards of the gas bubbles shear forces. This impeller supports high K La (mass transfer coefficient) values and is ideal for microcarrier cultures.

Foam - Direct sparging with gas and a high protein content of the medium are the two main culprits in foam production. The addition of antifoam agents via a peristaltic pump controlled by a foam sensor in the culture medium is common for microbial cultures, but is usually not considered in mammalian cell culture. The Cell Lift impeller also features a foam-elimination chamber and an optional Air Wash SystemTM to minimize foaming.


Combination Fermentor and Bioreactor

Given the major differences between microbial and mammalian culture needs, careful attention should be given to planning for future operations, because the cell type being used today may not be the one needed next year. To provide for changing requirements within the laboratory, it may be advantageous to consider a reactor designed for use both as a fermentor and as a dedicated cell culture system. The BioFlo 3000 fermentor (New Brunswick Scientific) provides the versatility necessary to accommodate the diverse requirements of virtually any microbial, yeast, animal, mammalian, insect or plant cell line. The benchtop reactor includes a built-in, interactive four -gas supplementation system with
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