Navigation Links
Scaling-Up from Spinners, T-Flasks & Shakers: A versatile bioreactor for mammalian and microbial cells

By Chris Julien; Reprinted with permission from American Biotechnology Laboratory.


Scaling up from spinners, T-flasks or shakers to a bioreactor system enables the researcher to reap the benefits of saving time. As an example of the typical savings one could expect, a single 5-L bioreactor has been shown to produce the equivalent amount of hybridoma cells as 150 250-mL T-flasks1. Moreover, a bioreactor requires only one laboratory technician and less than 2 ft of bench space for operation. The most significant decision when scaling up rests in selecting the equipment best suited to the users needs. Several factors must be considered, including the amount of product required (milligram or gram quantities); whether the end goal is the biomass itself or the products produced as a result of the culture; and whether the process uses an animal or microbial cell line, since the culture requirements and therefore the reactor design are inherently different for each.

This article focuses on the third factor, providing an overview of the most important differences between microbial culture and mammalian cell culture requirements, as well as describing a single reactor system capable of handling the varied needs of both.


Mammalian vs. Microbial Culture
Six basic areas in which culture requirements differ are growth rate, temperature, pH, dissolved oxygen, shear sensitivity, and foam. The following sections will discuss each of these areas in more detail.

Growth Rate - Growth rate, or the amount of time needed for cells to double, can be counted in minutes for microbes and upwards of a day for animal cells. A typical microbial culture may run just a few days to a week, and is therefore often run in batch mode. Because mammalian cultures will likely last several weeks, these systems are of
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. A technique for increasing yields in bioreactors and disposable cell-culture systems
2. Improving the accuracy and speed of mammalian cell counting
3. Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel
4. Embryonic stem cells, mouse
5. Primary cells, rat heart endothel
6. Primary cells, rat heart muscle
7. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
8. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
9. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
10. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
11. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
Post Your Comments:
(Date:7/31/2015)... July 31, 2015 In today,s fast ... help them drive positive health outcomes, improve profitability ... of patient care in their communities. At ThoughtSpot ... and conference, AmerisourceBergen announced a new set of ... Administration Organization and help independent pharmacies endure the ...
(Date:7/31/2015)... 2015 China Cord Blood Corporation (NYSE: CO) ... leading provider of cord blood collection, laboratory testing, hematopoietic ... announced that the Company has filed its Annual Report ... Commission. The filed Form 20-F includes audited financial statements ... Form 20-F can be accessed by visiting the U.S. ...
(Date:7/30/2015)... ... , ... Ralco is honored to announce that it is the primary sponsor ... August 5-9 in Marshall. The Ralco Enrichment Center is an interactive experience where the ... their daily lives. This unique exhibit also features a birthing center for fair goers ...
(Date:7/30/2015)... CAMBRIDGE, Mass. , July 30, 2015 /PRNewswire/ ... software solutions for the interpretation and analysis of ... Matthew Fischer has joined the company ... Photo - http://photos.prnewswire.com/prnh/20150730/250931 ... astounding capabilities to integrate, interpret, analyze and explore ...
Breaking Biology Technology:AmerisourceBergen Unveils Suite of Enhanced Capabilities for Independent Pharmacies at ThoughtSpot 2015 2AmerisourceBergen Unveils Suite of Enhanced Capabilities for Independent Pharmacies at ThoughtSpot 2015 3China Cord Blood Corporation Files Its Annual Report on Form 20-F 2Ralco Announces Primary Sponsorship of Ralco Enrichment Center at Lyon County Fair 2GenoSpace Introduces Matthew Fischer as Vice President of Sales and Marketing 2
... Data into Gems of Knowledge Using discoveryHub™ ... , This paper discusses key problems and opportunities created ... complexity of life science data available today, and presents ... enables rapid ‘harvesting’ and analysis of critical scientific data ...
... , ... • Alexa Fluor • TAMRA • ... Flexible and scalable uHTS programs demand that organizations ... the task. This application note describes experiments to assess the performance of ...
... Key words: H ... SPA Imaging Beads , , ... important pathophysiological roles in central and peripheral tissues. Generally released from stimulated ... (1).The function of histamine is mediated via four G protein-coupled receptors, H ...
Cached Biology Technology:Exploiting the Life Science Data Explosion to Speed New Drug Discovery 2Exploiting the Life Science Data Explosion to Speed New Drug Discovery 3Exploiting the Life Science Data Explosion to Speed New Drug Discovery 4Exploiting the Life Science Data Explosion to Speed New Drug Discovery 5Exploiting the Life Science Data Explosion to Speed New Drug Discovery 6Exploiting the Life Science Data Explosion to Speed New Drug Discovery 7Exploiting the Life Science Data Explosion to Speed New Drug Discovery 8Exploiting the Life Science Data Explosion to Speed New Drug Discovery 9Exploiting the Life Science Data Explosion to Speed New Drug Discovery 10Exploiting the Life Science Data Explosion to Speed New Drug Discovery 11Exploiting the Life Science Data Explosion to Speed New Drug Discovery 12Exploiting the Life Science Data Explosion to Speed New Drug Discovery 13Exploiting the Life Science Data Explosion to Speed New Drug Discovery 14Exploiting the Life Science Data Explosion to Speed New Drug Discovery 15Exploiting the Life Science Data Explosion to Speed New Drug Discovery 16Exploiting the Life Science Data Explosion to Speed New Drug Discovery 17Performance of fluorescent dyes with LEADseeker Multimodality Imaging System 2Performance of fluorescent dyes with LEADseeker Multimodality Imaging System 3Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System 2Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System 3Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System 4Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System 5
(Date:7/31/2015)... Kina, 31. juli 2015 Den 10. internationale ... af BGI fra den 22. - 25. oktober i ... fejrer sin 10-års fødselsdag i år. Siden starten i ... årlige møder på ,omik,-feltet, og er en af de ... ICG-10 fokuserer på nylige gennembrud og fremskridt inden ...
(Date:7/31/2015)... , 31 de julio de 2015 ... (ICG-10,  www.icg-10.org ) del 22 al 25 de octubre ... La conferencia celebra su décimo aniversario este ... convertido en una de las reuniones anuales más influyentes ... es una de las conferencias científicas más dinámicas, entusiastas ...
(Date:7/31/2015)... de 2015 A 10 a. Conferência ... pela BGI de 22-25 de outubro de 2015, em ... conferência está celebrando seu 10 o. aniversário este ano. ... uma das reuniões mais influentes do mundo no ramo ... entusiastas e prazerosos. A ICG-10 se ...
Breaking Biology News(10 mins):Den 10. internationale konference om Genomics (ICG-10) åbner i oktober 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 3A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 2A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 3
... sexes, just so-called mating types. A new study being ... are great similarities between the parts of DNA that determine ... DNA that determine mating types in certain fungi. This ... the evolutionary development of sex chromosomes. In the plant ...
... eye diseases and leading causes of blindnessage-related macular degeneration ... drugs that activate a protein found in blood vessel ... Medicine and several other institutions have announced in a ... and even reversed when the protein, Robo4, was activated ...
... available in German . In roughly five to ... of breast cancer i.e., hereditary and, thus, genetic factors play a ... are a major cause of familial breast cancer these are ... Germany, 75 percent of familial breast cancers are not attributable to ...
Cached Biology News:Fungi can tell us about the origin of sex chromosomes 2Blood vessel protein reverses macular degeneration, diabetic retinopathy in mice 2Blood vessel protein reverses macular degeneration, diabetic retinopathy in mice 3Gene variant increases breast cancer risk 2
IMAGEQUANT 100 IQTL, 1 EA. Category: ImageQuant CCD Imagers....
ImageQuant 100 UV, 1 EA. Category: ImageQuant CCD Imagers....
The FlowTACS Apoptosis Detection Kit is designed specifically for in situdetection of apoptotic...
...
Biology Products: