Navigation Links
Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

r automation in mind. It is possible that the two kits could be fully automated to run sequentially. However, storage and transport solutions would need to be considered for this system integration. This is another aspect of "scalability", in that the semi-automated system presented here could be integrated into a fully automated system. The MJ Research Hard-ShellThin Wall plates were chosen for their rigidity before and after thermocycling. Plates that maintain constant physical dimensions are crucial for automation.

Travel entry and exit speeds of 20% and 5% were chosen based on observations where rapid entry and exit can cause non-uniform droplet breakoff. Higher entry speeds would likely have little affect, however, exit speeds can have a dramatic affect on precision and accuracy, as evidenced by visual observation of droplet residue left on the outside of pipetting devices.

Tips are ejected immediately prior to loading in our protocols as a matter of programming style. This style takes into account the case where a user has paused or stopped a method with tips left on.

The precision and accuracy of the Sciclone ALH 3000 was sufficient to produce yields comparable to the manual results. Since the well-to-well %CV was <4.2 and the smallest volume transfer was 0.5 μL, it is reasonable to infer that the Sciclone ALH 3000 performed within its operating specifications for the types of liquids used in these kits, that the PCR reaction provides a leveling effect, or both.

Conclusions:

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000, produce data equivalent or superior to historical manual results3. Since all of this work was performed on an "out-of-the-box" system, it is likely that optimization of the system and liquid classes
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. RNAqueous-96 RNA Isolation Kit Automation Protocol
2. Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor
3. Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
4. Purified Single-Stranded M13 DNA for Automated Sequencing
5. Automated Fluorescent-Tag Cycle Sequencing
6. Eppendorf Mastercycler ABI Big Dye Sequencing Protocol
7. QuickLane 24 - 48 Hour Sequencing
8. cDNA Sequencing & Finishing
9. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
10. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
11. Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C
Post Your Comments:
(Date:4/16/2015)... Streetsboro, Ohio (PRWEB) April 16, 2015 ... Safety Index, overexertion involving an outside source was ... injuries. This includes injuries related to lifting, pushing, ... $15.1 billion in direct costs. The David Round ... Round is committed to working hard to develop ...
(Date:4/16/2015)... 16, 2015  In recognition of World Hemophilia Day, ... groups to illuminate more than 15 prominent landmarks red throughout ... Boston,s Zakim Bridge and Prudential Tower, ... Mercedes-Benz Superdome and Denver,s ... of April 17, and coincide with hemophilia community events in ...
(Date:4/16/2015)... , April 16, 2015 /CNW/ - Bioenterprise Corporation is ... newest office in Charlottetown , PEI.  ... Prince Edward Island ADAPT Council for the commercialization of ... forward to working with PEI ADAPT.  They have been ... more than a decade," explains Dave Smardon , ...
(Date:4/16/2015)... April 16, 2015  AnaptysBio, Inc., a leader ... antibodies, today announced the advancement of its ... IND-enabling studies. The Company,s anti-IL-36R therapeutic antibody, ... generalized pustular psoriasis (GPP), an orphan systemic ... is wholly owned within AnaptysBio,s proprietary antibody ...
Breaking Biology Technology:The David Round Company Helps Companies Meet OSHA Standards by Combining Safety, Ergonomics, and Productivity 2Biogen Shines A Light On Hemophilia Community By Illuminating U.S. Landmarks Red On World Hemophlia Day 2Biogen Shines A Light On Hemophilia Community By Illuminating U.S. Landmarks Red On World Hemophlia Day 3Biogen Shines A Light On Hemophilia Community By Illuminating U.S. Landmarks Red On World Hemophlia Day 4Bioenterprise Corporation Expands into the Maritimes 2AnaptysBio Advances First-In-Class Anti-IL-36 Receptor Antibody Program To Treat Orphan Inflammatory Disease 2
... 20, 2010) -- Recently, academic debate has been swirling ... the most ubiquitous of phenomena, including photosynthesis, the process ... particular, physicists have suggested that entanglement (the quantum interconnection ... atoms that are separated in physical space) could be ...
... ,, CHANTILLY, Va. , Aug. 6 ... as director of Chemical-Biological Defense Programs.  In this role, Chotani will lead ... U.S. Army and the Office of the Secretary of Defense, as well ... Health and the Centers for Disease Control. , ...
... Thursday, August 5, 2010 at 4:30 p.m. Eastern Time , ... BOTHELL, WA and VANCOUVER , Aug. 5 /PRNewswire-FirstCall/ - ... unaudited financial results for the three and six months ended June 30, 2010 and ... , , ...
Cached Biology Technology:TASC, Inc. Appoints Rashid Chotani to Lead Chemical-Biological Defense Programs 2TASC, Inc. Appoints Rashid Chotani to Lead Chemical-Biological Defense Programs 3OncoGenex Reports Second Quarter Financial Results 2OncoGenex Reports Second Quarter Financial Results 3OncoGenex Reports Second Quarter Financial Results 4OncoGenex Reports Second Quarter Financial Results 5OncoGenex Reports Second Quarter Financial Results 6OncoGenex Reports Second Quarter Financial Results 7OncoGenex Reports Second Quarter Financial Results 8OncoGenex Reports Second Quarter Financial Results 9
(Date:3/26/2015)... The Granite Club, ... club, today announced it has implemented a state-of-the-art access ... for members and staff, while restricting access to authorized ... selected FST,s IMID Access system because it has the ... and staff, in addition to unparalleled security," said ...
(Date:3/24/2015)... March 24, 2015   NexID Biometrics LLC, ... security-authentication needs, today announced the beginning of shipments of ... The company, based in Potsdam, ... three-day Connect:ID Expo, which began here today at the ... version 2.0 of its SDK boosts the accuracy rate ...
(Date:3/23/2015)... 2015   HOYOS Labs , a leading global ... that the Company will demonstrate multiple biometrics-based technologies under ... at Connect:ID on March 23 through 25, 2015, in ... highlight the IEEE Biometric Open Protocol Standard; free biometrics-based ... access control system. BOPS was invented by HOYOS Labs ...
Breaking Biology News(10 mins):Granite Club First Private Club in North America to Implement FST Biometrics Secure Access Solution, as Part of Multi-million Dollar Expansion 2NexID Begins Shipments Of Version 2.0 Fake-Finger-Detection Solution 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 3
... genes are especially challenging because they combine many ... of non-coding elements (introns). During processing, introns are ... a template for proteins called messenger RNA (mRNA). ... of a functional protein or, worse, produce an ...
... Institute of Dental and Craniofacial Research (NIDCR), part of ... new piece to the puzzle of how tumor cells ... abnormal growth, a well-known process called angiogenesis. , As ... Cell, the scientists found that in addition to the ...
... cancer that is based on infection with a specially designed ... has shown promise in clinical trials. Now, new research suggests ... as well. The study, published in the July issue of ... to viral therapy after they have been incubated at an ...
Cached Biology News:Carnegie Mellon University research reveals how cells process large genes 2Carnegie Mellon University research reveals how cells process large genes 3New lead reported in tumor angiogenesis 2New lead reported in tumor angiogenesis 3Elevated temperature enhances success of viral cancer therapy 2
HistoScan AP Universal Goat Kit...
4',6-Diamidino-2-phenylindole dihydrochloride...
No description available in Ensembl Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
Sheep polyclonal to Quinolone ( Abpromise for all tested applications). Antigen: Norfloxacin(N)conjugated to BSA...
Biology Products: