Navigation Links
Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

or TempliPhi Premix, which contains the active enzyme. These conditions were the primary concerns driving the decisions for selection of the automation presented in this note.

In addition, we addressed throughput scalability with a method that allows the user to select whether the Premix source will be a microcentrifuge tube or a column reservoir. Using IF/THEN type method control, at the beginning of the method a user can specify the Premix source container. For low throughput situations, the user may choose to use only a single microcentrifuge tube, while for higher throughput applications the user can specify a column reservoir with a higher capacity. Even higher capacity reservoirs would be simple to include in the method, however, we choose to do all our work in a single temperature controlled reservoir which has both tube and column options available. The enzyme in the Premix is the most costly component of the TempliPhi kit, and temperature control of the Premix is necessary to maintain enzyme activity.

Timing comparison results are presented for 96-well plate format for both tube and reservoir sources of Premix.

Materials and Methods

Instrumentation

Liquid Handler (Sciclone ALH 3000 with software version 3.1.14, firmware version 1.14 [CaliperLS]). The Sciclone was equipped with the following:

- Low Volume Head (P/N 103453/0)
- 96 Mandrel Array (P/N 103969/1)
- Press Station (P/N 104826/0)
- Z-8 Pipettor (P/N 102425/C)
- Temperature-Controlled Locator (P/N 103412)
- Temperature-Controlled Locator Block (P/N 103481), includes 3 column reservoirs and holds 12 microcentrifuge tubes.
Thermocycler (MJ Research PTC-200)
Capillary Sequencer (Amersham BioSciences MegaBACE 1000)

Materials:'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. RNAqueous-96 RNA Isolation Kit Automation Protocol
2. Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor
3. Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
4. Purified Single-Stranded M13 DNA for Automated Sequencing
5. Automated Fluorescent-Tag Cycle Sequencing
6. Eppendorf Mastercycler ABI Big Dye Sequencing Protocol
7. QuickLane 24 - 48 Hour Sequencing
8. cDNA Sequencing & Finishing
9. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
10. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
11. Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C
Post Your Comments:
(Date:7/30/2015)... 30, 2015 HIGHLIGHTS:Q2 2015 Results ... , Reported sales were $697 million compared to ... grew organically by 8%, and changes in foreign currency ... sales by 1%. , By business unit, organic ... and 11% in SAFC Commercial. , Reported diluted ...
(Date:7/30/2015)... ... July 30, 2015 , ... GEA's ... designed for continuous operation up to 1500 bar. The sanitary design gauge of ... preventing toxic contamination. , The Pony™ NS2006L homogenizer is an ideal solution for ...
(Date:7/29/2015)... According to a new market research report, ... , published by Mordor Intelligence, the Global market is expected ... 2020, with North America being the ... global market size. The Global market for Stem Cells is ... period of (2015-2020).      (Logo: http://photos.prnewswire.com/prnh/20150428/740799) , ...
(Date:7/29/2015)... /PRNewswire/ - BioAmber Inc. (NYSE: BIOA ) today announced ... Officer has resigned.  The Company has hired Andrew ... in December 2014, as full time interim CFO and ... Andy Ashworth began his employment today, ... transition.  Andy was the Company,s CFO from September 2011 ...
Breaking Biology Technology:Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 2Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 3Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 4Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 5Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 6Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 7Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 8Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 9Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 10Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 11Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 12Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 13Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 14Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 15Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 16Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 17Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 18Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 19Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 20GEA Announces the Pony™ NS2006L… a Self-contained, High Pressure Lab Homogenizer for Product Development Ensuring Maximum Efficiency and Safety 2Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 2Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 3BioAmber Announces Change in Chief Financial Officer 2BioAmber Announces Change in Chief Financial Officer 3
... ALBUQUERQUE, N.M -- Unexpected voltage increases of up to ... observed at Sandia National Laboratories., Designers of next-generation ... telephones, handheld computers, batteries and certain solar arrays ... "People have been working on nanowires for 20 years," ...
... /PRNewswire-iReach/ -- Z-Distribution is proud to introduce  Fytofontana ... most powerful weapons to fight and help reverse the ... natural process, no-one likes to see its signs, especially ... display most of the time. Plastic surgery, ...
... BALTIMORE, Dec. 7, 2011  Fortis now offers a ... at two Florida campuses – Fortis College, Cutler Bay ... Fortis is open to students who already have a ... required educational prerequisites. Fortis is now enrolling ...
Cached Biology Technology:Voltage increases up to 25 percent observed in closely packed nanowires at Sandia Labs 2Voltage increases up to 25 percent observed in closely packed nanowires at Sandia Labs 3Fytofontana Cosmeceuticals: The Swiss Secret For Incredibly Young And Beautiful Skin 2Fortis Offers Bachelor of Science in Nursing Degree Program to Florida Students 2Fortis Offers Bachelor of Science in Nursing Degree Program to Florida Students 3
(Date:7/31/2015)... , 31 de julho de 2015 A ... www.icg-10.org ) será realizada pela BGI de 22-25 de outubro ... A conferência está celebrando seu 10 o. ... a ICG se tornou uma das reuniões mais influentes ... encontros científicos mais dinâmicos, entusiastas e prazerosos. ...
(Date:7/31/2015)... China , 31 de julio de 2015 ... www.icg-10.org ) se celebrará por medio de BGI del 22 al ... . Este año, la conferencia celebra ... ICG se ha convertido en una de las reuniones anuales ... ser una de las reuniones más dinámicas, entusiastas y mejores ...
(Date:7/31/2015)... Jul. 31, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... focused on the growing mobile commerce market and creator ... filed provisional patent 62/198989 for ELECTRONIC CRYPTO-CURRENCY ... a method to advance crypto-currencies such as Bitcoin into ... common, uniform way to manage all payments.  ...
Breaking Biology News(10 mins):A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 2A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 3La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 2La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 3NXT-ID Patents Electronic Crypto-Currency Management Technology 2NXT-ID Patents Electronic Crypto-Currency Management Technology 3NXT-ID Patents Electronic Crypto-Currency Management Technology 4
... Big genes are especially challenging because they combine ... stretches of non-coding elements (introns). During processing, introns ... form a template for proteins called messenger RNA ... expression of a functional protein or, worse, produce ...
... are especially challenging because they combine many coding ... non-coding elements (introns). During processing, introns are snipped ... template for proteins called messenger RNA (mRNA). Mistakes ... a functional protein or, worse, produce an abnormal ...
... A therapeutic approach for battling cancer that is based on ... that causes the common cold has shown promise in clinical ... useful weapon in the fight as well. The study, published ... cells are even more sensitive to viral therapy after they ...
Cached Biology News:Reversal of role for a viral protein associated with the development of lymphoma 2Reversal of role for a viral protein associated with the development of lymphoma 3Carnegie Mellon University research reveals how cells process large genes 2Carnegie Mellon University research reveals how cells process large genes 3Elevated temperature enhances success of viral cancer therapy 2
The Hybrid Hunter Yeast Two-Hybrid System uses the activation of reporter genes to indicate positive protein-protein interactions. Two reporters are used an auxotrophic marker and lacZ....
Request Info...
Sprague Dawley rats, 7-8 weeks of age, mixed gender; collected by exsanguination in Alsever's solution at 1:2 dilution...
2-min, single-step add-mix-measure assay for luciferase expression and HTS in mammalian cells. Linear detection range: 2 fg to 46 ng luciferase....
Biology Products: