Please refer to the current literature for information on the cleaning
and use of peptides.
A concentration of at least 510 g/ml should be used
for injection, because some peptides rapidly degrade in the cells
Purification The purification of oligonucleotide solutions is very
important. Cleaning with gel or HPLC is recommended.
Like DNA, oligonucleotides are dissolved in bidist. water after
A concentration of 12 g/ml should be used for injection
of antisense oligonucleotides with 1020 bases.
Note: Injected oligos accumulate easily in the nucleus.
 Pepperkok, R., Schneider, C., Philipson, L., and Ansorge, W. (1988).
Single cell assay with an automated capillary microinjection system.
Exp.Cell Res. 178, 369-376.
 Pepperkok, R., Scheel, J., Horstmann, H.,Hauri, H.P., Griffiths,
G., and Kreis,T. E. (1993b). -COP is essential for biosynthetic
membrane transport from the endplasmic reticulium to the Golgi complex
in vivo. Cell 74, 71-82.
 Pepperkok, R., Saffrich, R., and Ansorge, W. (1994). Computer-Automated
Capillary Microinjection of Macromolecules into Living Cells. Cell
Biology: A Laboratory Handbook, CSH Laboratory Press
 Antibodies: A laboratory manual (1988). Harlow, E. and Lane, D.
ed., CSH Laboratory Press.
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