Navigation Links
Sample Preparation for Microinjection

art to agglutinate leading to blockages of the injection capillaries. Defrosting should be performed as quickly and gently as possible Before loading the capillary, centrifuge material for 15 minutes at 4C (10,000 x g). Chill supernatant or load directly into capillary.

DNA [5] Purification The highest expression level of microinjected plasmid DNA is achieved when the DNA has been purified by CsCl ethidium bromide gradient centrifugation in accordance with the Maniatis protocol [6].

Dissolve DNA in bidist. water after purification.

Buffers, e.g., PBs, are not recommended as this often leads to blockages of the capillaries.

DNA concentrations of 20 g/ml to 200 g/ml for plasmid DNA injection has been recommended [1].

Storage Store at 20C in small aliquots of 510 l.

Defrosting should be performed as quickly and gently as possible.

Before loading the capillary, centrifuge material for 15 minutes at 4C (10,000 x g). Chill supernatant or load directly into capillary.

Repeated centrifugation does not cause damage but is not necessary for each capillary filling when chilled with ice and used within an hour.

RNA Purification Any standard protocol is suitable for purifying RNA solutions [6].


'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA
2. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
3. A Better Way to Isolate RNA AND Protein From the Same Sample
4. Isolate Total RNA and Protein From the Same Sample
5. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
6. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
7. RNA from LCM Samples
8. RNA, DNA, and Protein from a Single Sample
9. Robust High Throughput RNA Isolation from Blood Samples
10. RNAlater Around the World: The Art of Processing Difficult Samples
11. Working With Laser Capture Microdissection Samples
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Sample Preparation for Microinjection

(Date:1/15/2014)... 15, 2014 Two champions of science, ... sponsorship of an annual competition for middle and high ... to, innovative STEM study. The competition presents students with ... of Engineering Aptitude, Mathematics, and Sciences is a ...
(Date:1/15/2014)... Freeslate, Inc ., the leading provider of ... one of India’s top five pharmaceutical companies, has received ... for high throughput solid form screening. , Lupin, ... range of quality, affordable generic and branded formulations and ...
(Date:1/14/2014)... 2014 Carahsoft and CDS Federal Services ... 2014 at 2pm EST (11am PST), “Natural Language Processing: ... on how technology can turn raw, heterogeneous data into ... agencies. The online webinar will last approximately one hour. ...
(Date:1/14/2014)... 2014 EquitiesIQ, a leading informational research ... Alliqua is an emerging biomedical company acquiring, developing, manufacturing, ... market. , Free report download: http://equitiesiq.com/reports/alliqua/ , ... management team and Board, which launched the company’s new ...
Breaking Biology Technology:Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 5Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3
... Mental Health Programs Supported by a Grant from Otsuka ... America Pharmaceutical, Inc., ... named three nonprofit organizations and one individual,as the recipients of the ... presented at the 2008 APF Annual Benefit in,Washington, D.C. Each organization ...
... the patient,s own whole blood, SOMERS, Conn., May ... the health professionals who manage patients on heart and,lung ... Blood,Resources, online waste calculator can be used to estimate ... machines most commonly used,during heart surgery to salvage the ...
... and some clever chemistry, University of California, Berkeley, researchers ... the sugar coating on their cells. , This novel ... that coat cells is a new tool for those ... because its transparent embryos allow easy observation of living ...
Cached Biology Technology:American Psychiatric Foundation Announces Recipients of 2008 Awards for Advancing Minority Mental Health 2American Psychiatric Foundation Announces Recipients of 2008 Awards for Advancing Minority Mental Health 3American Psychiatric Foundation Announces Recipients of 2008 Awards for Advancing Minority Mental Health 4American Psychiatric Foundation Announces Recipients of 2008 Awards for Advancing Minority Mental Health 5Global Blood Resources' On-Line Calculator Reveals Wasted Blood Costs by Blood Salvage Machines During Heart Surgery to be in the Billions 2Global Blood Resources' On-Line Calculator Reveals Wasted Blood Costs by Blood Salvage Machines During Heart Surgery to be in the Billions 3Glowing zebrafish help researchers track role of sugars in the cell 2Glowing zebrafish help researchers track role of sugars in the cell 3
(Date:4/17/2014)... Clemson professor Rajendra Singh Thursday as a "Champion ... expand solar deployment in the residential, commercial and ... Professor of Electrical and Computer Engineering and director ... a local hero leading the charge across the ... solar power and driving policy changes at the ...
(Date:4/17/2014)... on a novel method to help kidney stone ... effective treatment possible., Kidney stones represent a major ... If left untreated, apart from being particularly painful, ... complications. In many patients treated successfully, stone recurrence ... pathological approach to diagnosis and treatment needs to ...
(Date:4/17/2014)... researchers have detected new early-warning signs of the potential ... have far-reaching implications for the diagnosis and treatment of ... million Americans. , "We had not expected to see ... stages," said Ann Elsner, professor and associate dean in ... the study. "We set out to study the early ...
Breaking Biology News(10 mins):White House honors Clemson professor as 'Champion of Change' for solar deployment 2New technique detects microscopic diabetes-related eye damage 2New technique detects microscopic diabetes-related eye damage 3
... step in the ongoing battle against secondhand tobacco smoke. They ... immediately detect the presence of secondhand smoke and even third-hand ... about the size of a matchbox car, the device uses ... A sensor chip then records the data on an SD ...
... University] A key advance, newly reported by chemists ... cheaper and more sustainable way to make acrylate, an ... fabrics to diapers. Chemical companies churn out billions ... propylene, a compound derived from crude oil. "What we,re ...
... after the FDA approved Kalydeco (Vx-770), the first drug of ... University of Missouri researchers believe they have found exactly how ... the future. Described in the current issue of the ... have redefined a key regulatory process in the defective protein ...
Cached Biology News:Scientists create new tools for battling secondhand smoke 2Scientists create new tools for battling secondhand smoke 3Breakthrough could lead to cheaper, more sustainable chemical production 2Discovery could increase efficacy of promising cystic fibrosis drug 2Discovery could increase efficacy of promising cystic fibrosis drug 3Discovery could increase efficacy of promising cystic fibrosis drug 4
... This kit allows for quick and reproducible immunoprecipitation ... The system is more reproducible than regular IPs, ... protein A/G agarose without disrupting the agarose bed. ... and Release is reversible, and elution of the ...
... Polyclonal Antibody, Unconjugated ... a dilution of 1/100. IP: Use ... Use at an assay dependent dilution. ... tested in other applications. Optimal ...
Membrane Proteins from over 50 different human Alzheimer, Diabetic, Liver Cirrhosis, and Lupus diseased tissues are available....
... Using multicolor analysis in a modern flow ... cells that are differentially labeled with fluorescent ... dyes typically used for cell labeling are ... by a flow cytometers filters. Thus, the ...
Biology Products: