Navigation Links
Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A

ware and spots of interest were cut from the gels using the ProteomeWorks spot cutter. Peptide mass fingerprint data was obtained from excised 2-D gel spots using the MassPREP station robotics system for protein digestion and MALDI target spotting. Gel spots were destained, reduced, alkylated, dried, digested with modified trypsin (Promega) while heated at 37C for 5 hr, and extracted with an acidic solution. Peptides were then automatically spotted onto MALDI target plates mixed with a matrix solution of 2 mg/ml -cyano-4-hydroxycinnamic acid. MALDI mass spectra were acquired on a M@LDI-LR instrument in reflector mode. A nitrogen laser (= 337 nm, Laser Science) was pulsed at 20 Hz. Ions were accelerated to 15 kV after a timelag focusing pulse of 2,750 V at 500 ns. Data were collected from the sample well and a near point lock mass (ACTH clip 1839, Sigma). The MALDI mass spectra were processed to identify monoisotopic peaks using the Micromass algorithm MaxEntLite. The search engine used to identify peptide mass fingerprint data was ProteinLynx Global SERVER 2.0 software.

Results
Three separate sample preparation protocols were used to fractionate and enrich mouse liver protein samples prior to 2DE. Although the proteins for each gel were prepared from the same starting material, the final gel results following fractionation provide completely different views of the mouse liver proteome (Figure 1). Proteins that were unique to the fractionated sample were selected for identification. Table 1 shows selected proteins from these gels that are associated with the cellular structures enriched for in the sample separation protocols. Many of these identified proteins are membrane associated.
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Sample Preparation for Microinjection
2. GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. A Better Way to Isolate RNA AND Protein From the Same Sample
5. Isolate Total RNA and Protein From the Same Sample
6. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
7. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
8. RNA from LCM Samples
9. RNA, DNA, and Protein from a Single Sample
10. Robust High Throughput RNA Isolation from Blood Samples
11. RNAlater Around the World: The Art of Processing Difficult Samples
Post Your Comments:
(Date:7/10/2014)... , July 10, 2014 On ... Dmitry Medvedev , presented Russia,s ... BIOCAD . The Company is developing a unique project ... project BIOCAD develops a number of innovative drugs based ... diseases. The ceremony took place at the International Exhibition ...
(Date:7/10/2014)... 2014 /PRNewswire-iReach/ -- Tute Genomics, the leader ... an agreement with Lineagen, Inc., to provide ... Dx PLUS.  Lineagen, focused on the ... currently offers FirstStep Dx PLUS, a ... by healthcare providers as a first-line genetic ...
(Date:7/10/2014)... THOUSAND OAKS, Calif., July 10, 2014   Ceres, ... biotechnology and seed company, announced today financial results for ... an update on its business. Ceres reported ... progress in product performance this growing season in Brazil, ... that affected the company,s sorghum evaluation areas for part ...
(Date:7/10/2014)... Adelaide research may help in the fight against terrorism ... tiny quantities of explosives with the use of light ... Sensors and Actuators B: Chemical , the researchers ... explosives in concentrations as low as 6.3 ppm (parts ... a few minutes. , "Traditionally explosives detection has involved ...
Breaking Biology Technology:Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 3Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9Detecting trace amounts of explosives with light 2
... developed a model that will allow them to simulate how ... model enables further research that may provide insight into the ... published each year that use the rat whisker system as ... move their whiskers rhythmically against objects to explore the environment ...
... Precision Therapeutics, Inc. announced that the launch of ... the personalization of cancer treatments.  BioSpeciFx® is composed ... clinically useful biomarker tests that identify critical molecular ... information generated by BioSpeciFx® in combination with Precision,s ...
... April 7, 2011 The Center for Business Intelligence (CBI) ... American Red Cross in support of the relief efforts in ... personal contributions to the fund while the organization matched employee ... and generosity CBI employees have displayed to help a nation ...
Cached Biology Technology:New model of whiskers provides insight into sense of touch 2New model of whiskers provides insight into sense of touch 3Precision Therapeutics Announces New Tumor Profiling Product to Augment Utility of ChemoFx® and Further Personalize Cancer Treatment 2The Center for Business Intelligence Donates to the American Red Cross, Matching Employee Contributions for Disaster Relief in Japan 2
(Date:7/10/2014)... -- Fingerprint Cards, (FPC,s) new mobile touch sensor ... Chinese Top 5 smartphone OEM has selected FPC1021 for a ... October 2014.   FPC is proud to announce ... has a planned date for the start of mass production ... phone of 3 million units. The Chinese OEM wants to ...
(Date:7/10/2014)... Fingerprint Cards, (FPC,s) new ... (DW). An Asian OEM has selected FPC1021 for a flagship ... August 2014.   FPC is proud to announce ... planned date for start of mass production in August 2014. ... 5M, for delivery in the first half of July. The ...
(Date:7/10/2014)... 9, 2014 The widespread adoption of ... part by meaningful use and related federal programs, ... with laboratory information. Now, more than ever, pathologists ... clinical informatics in order to deliver high quality ... the educational needs of pathologists in this evolving ...
Breaking Biology News(10 mins):FPC Wins DW for 1021 Touch Sensor for Flagship Smartphone from Chinese Top 5 Smartphone OEM 2FPC Wins DW and Initial Ramp Order for FPC1021 Touch Sensor for Flagship Smartphone from Asian OEM 2New Clinical Informatics Curriculum Prepares Future Pathologists 2New Clinical Informatics Curriculum Prepares Future Pathologists 3New Clinical Informatics Curriculum Prepares Future Pathologists 4
... pollen next, you have to literally think like one. , ... are more likely to stick with it rather than invest ... student at the University of Wisconsin-Milwaukee (UWM). , Flanigan ... bees as they gather pollen ?which plant species the bees ...
... five-year, $1 million grant from the National Cancer Institute, a ... improve the effectiveness of a drug that he developed ... Waldemar Debinski, M.D., Ph.D., pioneered a method to destroy cells ... The drug is currently being tested in clinical trials and ...
... research team led by scientists at the Broad Institute of ... kind of genetic "roadmap" that can connect human diseases with ... new drugs work in human cells. Called the "Connectivity Map," ... September 29 issue of Science and in separate publications in ...
Cached Biology News:Researchers find that bumblebees' flower choice matters 2Researchers find that bumblebees' flower choice matters 3Researchers find that bumblebees' flower choice matters 4Scientist works to improve treatment for brain tumors 2Genetic 'roadmap' charts links between drugs and human disease 2Genetic 'roadmap' charts links between drugs and human disease 3
... is the only PCR system that ensures ... time. It provides accuracy, consistency, and dependability ... the property of the DNA templates. This ... a unique blend of thermostable enzymes that ...
Special grade: for molecular biology Density: 1.000 g/mL (20 C)...
... to meet the needs of ... component-free medium supports fast cell ... densities, while maintaining high cell ... production using the Baculovirus Expression ...
... This CLS number ... number, created to easily ... If showing no availability ... the old Sigma-Aldrich number ...
Biology Products: